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Survey of major histocompatibility complex class II diversity in pig-tailed macaques

Pig-tailed macaques ( Macaca nemestrina ) serve as important models for human infectious disease research. Major histocompatibility complex (MHC) class II molecules are important to this research since they present peptides to CD4+ T cells. Despite the importance of characterizing the MHC-II alleles... Full description

Journal Title: Immunogenetics 2014, Vol.66(11), pp.613-623
Main Author: Karl, Julie
Other Authors: Heimbruch, Katelyn , Vriezen, Claire , Mironczuk, Cassandra , Dudley, Dawn , Wiseman, Roger , O’Connor, David
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0093-7711 ; E-ISSN: 1432-1211 ; DOI: 10.1007/s00251-014-0797-y
Link: http://dx.doi.org/10.1007/s00251-014-0797-y
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recordid: springer_jour10.1007/s00251-014-0797-y
title: Survey of major histocompatibility complex class II diversity in pig-tailed macaques
format: Article
creator:
  • Karl, Julie
  • Heimbruch, Katelyn
  • Vriezen, Claire
  • Mironczuk, Cassandra
  • Dudley, Dawn
  • Wiseman, Roger
  • O’Connor, David
subjects:
  • Macaca nemestrina
  • Major histocompatibility complex
  • MHC class II alleles
  • Next-generation sequencing
ispartof: Immunogenetics, 2014, Vol.66(11), pp.613-623
description: Pig-tailed macaques ( Macaca nemestrina ) serve as important models for human infectious disease research. Major histocompatibility complex (MHC) class II molecules are important to this research since they present peptides to CD4+ T cells. Despite the importance of characterizing the MHC-II alleles expressed in model species like pig-tailed macaques, to date, less than 150 MHC-II alleles have been named for the six most common classical class II loci ( DRA , DRB , DQA , DQB , DPA , and DPB ) in this population. Additionally, only a small percentage of these alleles are full-length, making it impossible to use the known sequence for reagent development. To address this, we developed a fast, high-throughput method to discover full-length MHC-II alleles and used it to characterize alleles in 32 pig-tailed macaques. By this method, we identified 128 total alleles across all six loci. We also performed an exon 2-based genotyping assay to validate the full-length sequencing results; this genotyping assay could be optimized for use in determining MHC-II allele frequencies in large cohorts of pig-tailed macaques.
language: eng
source:
identifier: ISSN: 0093-7711 ; E-ISSN: 1432-1211 ; DOI: 10.1007/s00251-014-0797-y
fulltext: fulltext
issn:
  • 1432-1211
  • 14321211
  • 0093-7711
  • 00937711
url: Link


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titleSurvey of major histocompatibility complex class II diversity in pig-tailed macaques
creatorKarl, Julie ; Heimbruch, Katelyn ; Vriezen, Claire ; Mironczuk, Cassandra ; Dudley, Dawn ; Wiseman, Roger ; O’Connor, David
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subjectMacaca nemestrina ; Major histocompatibility complex ; MHC class II alleles ; Next-generation sequencing
descriptionPig-tailed macaques ( Macaca nemestrina ) serve as important models for human infectious disease research. Major histocompatibility complex (MHC) class II molecules are important to this research since they present peptides to CD4+ T cells. Despite the importance of characterizing the MHC-II alleles expressed in model species like pig-tailed macaques, to date, less than 150 MHC-II alleles have been named for the six most common classical class II loci ( DRA , DRB , DQA , DQB , DPA , and DPB ) in this population. Additionally, only a small percentage of these alleles are full-length, making it impossible to use the known sequence for reagent development. To address this, we developed a fast, high-throughput method to discover full-length MHC-II alleles and used it to characterize alleles in 32 pig-tailed macaques. By this method, we identified 128 total alleles across all six loci. We also performed an exon 2-based genotyping assay to validate the full-length sequencing results; this genotyping assay could be optimized for use in determining MHC-II allele frequencies in large cohorts of pig-tailed macaques.
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titleSurvey of major histocompatibility complex class II diversity in pig-tailed macaques
descriptionPig-tailed macaques ( Macaca nemestrina ) serve as important models for human infectious disease research. Major histocompatibility complex (MHC) class II molecules are important to this research since they present peptides to CD4+ T cells. Despite the importance of characterizing the MHC-II alleles expressed in model species like pig-tailed macaques, to date, less than 150 MHC-II alleles have been named for the six most common classical class II loci ( DRA , DRB , DQA , DQB , DPA , and DPB ) in this population. Additionally, only a small percentage of these alleles are full-length, making it impossible to use the known sequence for reagent development. To address this, we developed a fast, high-throughput method to discover full-length MHC-II alleles and used it to characterize alleles in 32 pig-tailed macaques. By this method, we identified 128 total alleles across all six loci. We also performed an exon 2-based genotyping assay to validate the full-length sequencing results; this genotyping assay could be optimized for use in determining MHC-II allele frequencies in large cohorts of pig-tailed macaques.
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abstractPig-tailed macaques ( Macaca nemestrina ) serve as important models for human infectious disease research. Major histocompatibility complex (MHC) class II molecules are important to this research since they present peptides to CD4+ T cells. Despite the importance of characterizing the MHC-II alleles expressed in model species like pig-tailed macaques, to date, less than 150 MHC-II alleles have been named for the six most common classical class II loci ( DRA , DRB , DQA , DQB , DPA , and DPB ) in this population. Additionally, only a small percentage of these alleles are full-length, making it impossible to use the known sequence for reagent development. To address this, we developed a fast, high-throughput method to discover full-length MHC-II alleles and used it to characterize alleles in 32 pig-tailed macaques. By this method, we identified 128 total alleles across all six loci. We also performed an exon 2-based genotyping assay to validate the full-length sequencing results; this genotyping assay could be optimized for use in determining MHC-II allele frequencies in large cohorts of pig-tailed macaques.
copBerlin/Heidelberg
pubSpringer Berlin Heidelberg
doi10.1007/s00251-014-0797-y
pages613-623
date2014-11