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Novel MHC class I full-length allele and haplotype characterization in sooty mangabeys

Sooty mangabeys ( Cercocebus atys ) are natural SIV hosts and the presumed source of HIV-2 and SIVmac, which makes them a valuable model for HIV/SIV research. However, like other African primates, little is known about their major histocompatibility complex (MHC) genetics. In this study, we used Roc... Full description

Journal Title: Immunogenetics 2015, Vol.67(8), pp.437-445
Main Author: Heimbruch, Katelyn
Other Authors: Karl, Julie , Wiseman, Roger , Dudley, Dawn , Johnson, Zach , Kaur, Amitinder , O’Connor, David
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0093-7711 ; E-ISSN: 1432-1211 ; DOI: 10.1007/s00251-015-0847-0
Link: http://dx.doi.org/10.1007/s00251-015-0847-0
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recordid: springer_jour10.1007/s00251-015-0847-0
title: Novel MHC class I full-length allele and haplotype characterization in sooty mangabeys
format: Article
creator:
  • Heimbruch, Katelyn
  • Karl, Julie
  • Wiseman, Roger
  • Dudley, Dawn
  • Johnson, Zach
  • Kaur, Amitinder
  • O’Connor, David
subjects:
  • MHC class I
  • Sooty mangabey
  • SIV natural host
  • Haplotyping
  • Deep sequencing
ispartof: Immunogenetics, 2015, Vol.67(8), pp.437-445
description: Sooty mangabeys ( Cercocebus atys ) are natural SIV hosts and the presumed source of HIV-2 and SIVmac, which makes them a valuable model for HIV/SIV research. However, like other African primates, little is known about their major histocompatibility complex (MHC) genetics. In this study, we used Roche/454 and Illumina MiSeq deep sequencing in order to determine the MHC class I transcripts in a cohort of 165 sooty mangabeys from the Yerkes National Primate Research Center (YNPRC). We have characterized 121 functionally full-length classical ( Ceat - A and Ceat - B ) and non-classical ( Ceat - F and Ceat- I ) alleles and have also identified 22 Ceat - A / Ceat - B haplotype chromosomal combinations. We correlated these Ceat - A / Ceat - B haplotype combinations to recently described microsatellite haplotypes from the YNPRC colony. These newly identified alleles and haplotypes establish a resource for studying cellular immunity in sooty mangabeys and provide a framework for rapidly cataloging MHC class I sequences in an understudied, yet important, nonhuman primate species.
language: eng
source:
identifier: ISSN: 0093-7711 ; E-ISSN: 1432-1211 ; DOI: 10.1007/s00251-015-0847-0
fulltext: fulltext
issn:
  • 1432-1211
  • 14321211
  • 0093-7711
  • 00937711
url: Link


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titleNovel MHC class I full-length allele and haplotype characterization in sooty mangabeys
creatorHeimbruch, Katelyn ; Karl, Julie ; Wiseman, Roger ; Dudley, Dawn ; Johnson, Zach ; Kaur, Amitinder ; O’Connor, David
ispartofImmunogenetics, 2015, Vol.67(8), pp.437-445
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subjectMHC class I ; Sooty mangabey ; SIV natural host ; Haplotyping ; Deep sequencing
descriptionSooty mangabeys ( Cercocebus atys ) are natural SIV hosts and the presumed source of HIV-2 and SIVmac, which makes them a valuable model for HIV/SIV research. However, like other African primates, little is known about their major histocompatibility complex (MHC) genetics. In this study, we used Roche/454 and Illumina MiSeq deep sequencing in order to determine the MHC class I transcripts in a cohort of 165 sooty mangabeys from the Yerkes National Primate Research Center (YNPRC). We have characterized 121 functionally full-length classical ( Ceat - A and Ceat - B ) and non-classical ( Ceat - F and Ceat- I ) alleles and have also identified 22 Ceat - A / Ceat - B haplotype chromosomal combinations. We correlated these Ceat - A / Ceat - B haplotype combinations to recently described microsatellite haplotypes from the YNPRC colony. These newly identified alleles and haplotypes establish a resource for studying cellular immunity in sooty mangabeys and provide a framework for rapidly cataloging MHC class I sequences in an understudied, yet important, nonhuman primate species.
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titleNovel MHC class I full-length allele and haplotype characterization in sooty mangabeys
descriptionSooty mangabeys ( Cercocebus atys ) are natural SIV hosts and the presumed source of HIV-2 and SIVmac, which makes them a valuable model for HIV/SIV research. However, like other African primates, little is known about their major histocompatibility complex (MHC) genetics. In this study, we used Roche/454 and Illumina MiSeq deep sequencing in order to determine the MHC class I transcripts in a cohort of 165 sooty mangabeys from the Yerkes National Primate Research Center (YNPRC). We have characterized 121 functionally full-length classical ( Ceat - A and Ceat - B ) and non-classical ( Ceat - F and Ceat- I ) alleles and have also identified 22 Ceat - A / Ceat - B haplotype chromosomal combinations. We correlated these Ceat - A / Ceat - B haplotype combinations to recently described microsatellite haplotypes from the YNPRC colony. These newly identified alleles and haplotypes establish a resource for studying cellular immunity in sooty mangabeys and provide a framework for rapidly cataloging MHC class I sequences in an understudied, yet important, nonhuman primate species.
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abstractSooty mangabeys ( Cercocebus atys ) are natural SIV hosts and the presumed source of HIV-2 and SIVmac, which makes them a valuable model for HIV/SIV research. However, like other African primates, little is known about their major histocompatibility complex (MHC) genetics. In this study, we used Roche/454 and Illumina MiSeq deep sequencing in order to determine the MHC class I transcripts in a cohort of 165 sooty mangabeys from the Yerkes National Primate Research Center (YNPRC). We have characterized 121 functionally full-length classical ( Ceat - A and Ceat - B ) and non-classical ( Ceat - F and Ceat- I ) alleles and have also identified 22 Ceat - A / Ceat - B haplotype chromosomal combinations. We correlated these Ceat - A / Ceat - B haplotype combinations to recently described microsatellite haplotypes from the YNPRC colony. These newly identified alleles and haplotypes establish a resource for studying cellular immunity in sooty mangabeys and provide a framework for rapidly cataloging MHC class I sequences in an understudied, yet important, nonhuman primate species.
copBerlin/Heidelberg
pubSpringer Berlin Heidelberg
doi10.1007/s00251-015-0847-0
pages437-445
date2015-08