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Development and application of an oligonucleotide microarray for the detection of food-borne bacterial pathogens

The rapid and accurate detection and identification of food-borne pathogenic bacteria is critical for food safety. In this paper, we describe a rapid (

Journal Title: Applied Microbiology and Biotechnology 2007, Vol.76(1), pp.225-233
Main Author: Wang, Xin-Wei
Other Authors: Zhang, Liang , Jin, Lian-Qun , Jin, Min , Shen, Zhi-Qiang , An, Shuang , Chao, Fu-Huan , Li, Jun-Wen
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0175-7598 ; E-ISSN: 1432-0614 ; DOI: 10.1007/s00253-007-0993-x
Link: http://dx.doi.org/10.1007/s00253-007-0993-x
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recordid: springer_jour10.1007/s00253-007-0993-x
title: Development and application of an oligonucleotide microarray for the detection of food-borne bacterial pathogens
format: Article
creator:
  • Wang, Xin-Wei
  • Zhang, Liang
  • Jin, Lian-Qun
  • Jin, Min
  • Shen, Zhi-Qiang
  • An, Shuang
  • Chao, Fu-Huan
  • Li, Jun-Wen
subjects:
  • Oligonucleotide microarray
  • Detection
  • Bacterial pathogens
  • Food-borne
ispartof: Applied Microbiology and Biotechnology, 2007, Vol.76(1), pp.225-233
description: The rapid and accurate detection and identification of food-borne pathogenic bacteria is critical for food safety. In this paper, we describe a rapid (
language: eng
source:
identifier: ISSN: 0175-7598 ; E-ISSN: 1432-0614 ; DOI: 10.1007/s00253-007-0993-x
fulltext: fulltext
issn:
  • 1432-0614
  • 14320614
  • 0175-7598
  • 01757598
url: Link


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titleDevelopment and application of an oligonucleotide microarray for the detection of food-borne bacterial pathogens
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subjectOligonucleotide microarray ; Detection ; Bacterial pathogens ; Food-borne
descriptionThe rapid and accurate detection and identification of food-borne pathogenic bacteria is critical for food safety. In this paper, we describe a rapid (<4 h) high-throughput detection and identification system that uses universal polymerase chain reaction (PCR) primers to amplify a variable region of bacterial the 16S rRNA gene, followed by reverse hybridization of the products to species-specific oligonucleotide probes on a chip. This procedure was successful in discriminating 204 strains of bacteria from pure culture belonging to 13 genera of bacteria. When this method was applied directly to 115 strains of bacteria isolated from foods, 112/115 (97.4%) were correctly identified; two strains were indistinguishable due to weak signal, while one failed to produce a PCR product. The array was used to detect and successfully identify two strains of bacteria from food poisoning outbreak samples, giving results through hybridization that were identical to those obtained by traditional methods. The sensitivity of the microarray assay was 10 2  CFU of bacteria. Thus, the oligonucleotide microarray is a powerful tool for the detection and identification of pathogens from foods.
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titleDevelopment and application of an oligonucleotide microarray for the detection of food-borne bacterial pathogens
descriptionThe rapid and accurate detection and identification of food-borne pathogenic bacteria is critical for food safety. In this paper, we describe a rapid (<4 h) high-throughput detection and identification system that uses universal polymerase chain reaction (PCR) primers to amplify a variable region of bacterial the 16S rRNA gene, followed by reverse hybridization of the products to species-specific oligonucleotide probes on a chip. This procedure was successful in discriminating 204 strains of bacteria from pure culture belonging to 13 genera of bacteria. When this method was applied directly to 115 strains of bacteria isolated from foods, 112/115 (97.4%) were correctly identified; two strains were indistinguishable due to weak signal, while one failed to produce a PCR product. The array was used to detect and successfully identify two strains of bacteria from food poisoning outbreak samples, giving results through hybridization that were identical to those obtained by traditional methods. The sensitivity of the microarray assay was 10 2  CFU of bacteria. Thus, the oligonucleotide microarray is a powerful tool for the detection and identification of pathogens from foods.
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titleDevelopment and application of an oligonucleotide microarray for the detection of food-borne bacterial pathogens
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abstractThe rapid and accurate detection and identification of food-borne pathogenic bacteria is critical for food safety. In this paper, we describe a rapid (<4 h) high-throughput detection and identification system that uses universal polymerase chain reaction (PCR) primers to amplify a variable region of bacterial the 16S rRNA gene, followed by reverse hybridization of the products to species-specific oligonucleotide probes on a chip. This procedure was successful in discriminating 204 strains of bacteria from pure culture belonging to 13 genera of bacteria. When this method was applied directly to 115 strains of bacteria isolated from foods, 112/115 (97.4%) were correctly identified; two strains were indistinguishable due to weak signal, while one failed to produce a PCR product. The array was used to detect and successfully identify two strains of bacteria from food poisoning outbreak samples, giving results through hybridization that were identical to those obtained by traditional methods. The sensitivity of the microarray assay was 10 2  CFU of bacteria. Thus, the oligonucleotide microarray is a powerful tool for the detection and identification of pathogens from foods.
copBerlin/Heidelberg
pubSpringer-Verlag
doi10.1007/s00253-007-0993-x
pages225-233
date2007-08