schliessen

Filtern

 

Bibliotheken

Improvement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains

NADPH-dependent reactions play important roles in production of industrially valuable compounds. In this study, we used phosphofructokinase (PFK)-deficient strains to direct fructose-6-phosphate to be oxidized through the pentose phosphate pathway (PPP) to increase NADPH generation. pfkA or pfkB sin... Full description

Journal Title: Applied Microbiology and Biotechnology 2013, Vol.97(15), pp.6883-6893
Main Author: Wang, Yipeng
Other Authors: San, Ka-Yiu , Bennett, George
Format: Electronic Article Electronic Article
Language: English
Subjects:
PFK
.
ID: ISSN: 0175-7598 ; E-ISSN: 1432-0614 ; DOI: 10.1007/s00253-013-4859-0
Link: http://dx.doi.org/10.1007/s00253-013-4859-0
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: springer_jour10.1007/s00253-013-4859-0
title: Improvement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains
format: Article
creator:
  • Wang, Yipeng
  • San, Ka-Yiu
  • Bennett, George
subjects:
  • PFK
  • NADPH bioavailability
  • pfkA
  • pfkB
  • G6PDH
  • .
ispartof: Applied Microbiology and Biotechnology, 2013, Vol.97(15), pp.6883-6893
description: NADPH-dependent reactions play important roles in production of industrially valuable compounds. In this study, we used phosphofructokinase (PFK)-deficient strains to direct fructose-6-phosphate to be oxidized through the pentose phosphate pathway (PPP) to increase NADPH generation. pfkA or pfkB single deletion and double-deletion strains were tested for their ability to produce lycopene. Since lycopene biosynthesis requires many NADPH, levels of lycopene were compared in a set of isogenic strains, with the pfkA single deletion strain showing the highest lycopene yield. Using another NADPH-requiring process, a one-step reduction reaction of 2-chloroacrylate to 2-chloropropionic acid by 2-haloacrylate reductase, the pfkA pfkB double-deletion strain showed the highest yield of 2-chloropropionic acid product. The combined effect of glucose-6-phosphate dehydrogenase overexpression or lactate dehydrogenase deletion with PFK deficiency on NADPH bioavailability was also studied. The results indicated that the flux distribution of fructose-6-phosphate between glycolysis and the pentose phosphate pathway determines the amount of NAPDH available for reductive biosynthesis.
language: eng
source:
identifier: ISSN: 0175-7598 ; E-ISSN: 1432-0614 ; DOI: 10.1007/s00253-013-4859-0
fulltext: fulltext
issn:
  • 1432-0614
  • 14320614
  • 0175-7598
  • 01757598
url: Link


@attributes
ID1374331048
RANK0.06999999
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.1007/s00253-013-4859-0
sourceidspringer_jour
recordidTN_springer_jour10.1007/s00253-013-4859-0
sourcesystemPC
pqid1399284759
galeid344279615
display
typearticle
titleImprovement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains
creatorWang, Yipeng ; San, Ka-Yiu ; Bennett, George
ispartofApplied Microbiology and Biotechnology, 2013, Vol.97(15), pp.6883-6893
identifier
subjectPFK ; NADPH bioavailability ; pfkA ; pfkB ; G6PDH ; .
descriptionNADPH-dependent reactions play important roles in production of industrially valuable compounds. In this study, we used phosphofructokinase (PFK)-deficient strains to direct fructose-6-phosphate to be oxidized through the pentose phosphate pathway (PPP) to increase NADPH generation. pfkA or pfkB single deletion and double-deletion strains were tested for their ability to produce lycopene. Since lycopene biosynthesis requires many NADPH, levels of lycopene were compared in a set of isogenic strains, with the pfkA single deletion strain showing the highest lycopene yield. Using another NADPH-requiring process, a one-step reduction reaction of 2-chloroacrylate to 2-chloropropionic acid by 2-haloacrylate reductase, the pfkA pfkB double-deletion strain showed the highest yield of 2-chloropropionic acid product. The combined effect of glucose-6-phosphate dehydrogenase overexpression or lactate dehydrogenase deletion with PFK deficiency on NADPH bioavailability was also studied. The results indicated that the flux distribution of fructose-6-phosphate between glycolysis and the pentose phosphate pathway determines the amount of NAPDH available for reductive biosynthesis.
languageeng
source
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://dx.doi.org/10.1007/s00253-013-4859-0$$EView_full_text_in_Springer_(Subscribers_only)
search
creatorcontrib
0Wang, Yipeng, N.
1San, Ka-Yiu, N.
2Bennett, George, N.
titleImprovement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains
descriptionNADPH-dependent reactions play important roles in production of industrially valuable compounds. In this study, we used phosphofructokinase (PFK)-deficient strains to direct fructose-6-phosphate to be oxidized through the pentose phosphate pathway (PPP) to increase NADPH generation. pfkA or pfkB single deletion and double-deletion strains were tested for their ability to produce lycopene. Since lycopene biosynthesis requires many NADPH, levels of lycopene were compared in a set of isogenic strains, with the pfkA single deletion strain showing the highest lycopene yield. Using another NADPH-requiring process, a one-step reduction reaction of 2-chloroacrylate to 2-chloropropionic acid by 2-haloacrylate reductase, the pfkA pfkB double-deletion strain showed the highest yield of 2-chloropropionic acid product. The combined effect of glucose-6-phosphate dehydrogenase overexpression or lactate dehydrogenase deletion with PFK deficiency on NADPH bioavailability was also studied. The results indicated that the flux distribution of fructose-6-phosphate between glycolysis and the pentose phosphate pathway determines the amount of NAPDH available for reductive biosynthesis.
subject
0PFK
1NADPH bioavailability
2pfkA
3pfkB
4G6PDH
5.
general
010.1007/s00253-013-4859-0
1English
2Springer Science & Business Media B.V.
3SpringerLink
sourceidspringer_jour
recordidspringer_jour10.1007/s00253-013-4859-0
issn
01432-0614
114320614
20175-7598
301757598
rsrctypearticle
creationdate2013
addtitle
0Applied Microbiology and Biotechnology
1Appl Microbiol Biotechnol
searchscopespringer_journals_complete
scopespringer_journals_complete
lsr30VSR-Enriched:[pages, pqid, galeid]
sort
titleImprovement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains
authorWang, Yipeng ; San, Ka-Yiu ; Bennett, George
creationdate20130800
facets
frbrgroupid4276950254506869018
frbrtype5
languageeng
creationdate2013
topic
0Pfk
1Nadph Bioavailability
2Pfka
3Pfkb
4G6pdh
5.
collectionSpringerLink
prefilterarticles
rsrctypearticles
creatorcontrib
0Wang, Yipeng
1San, Ka-Yiu
2Bennett, George
jtitleApplied Microbiology And Biotechnology
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Wang
1San
2Bennett
aufirst
0Yipeng
1Ka-Yiu
2George
3N.
au
0Wang, Yipeng
1San, Ka-Yiu
2Bennett, George
atitleImprovement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains
jtitleApplied Microbiology and Biotechnology
stitleAppl Microbiol Biotechnol
risdate201308
volume97
issue15
spage6883
epage6893
issn0175-7598
eissn1432-0614
genrearticle
ristypeJOUR
abstractNADPH-dependent reactions play important roles in production of industrially valuable compounds. In this study, we used phosphofructokinase (PFK)-deficient strains to direct fructose-6-phosphate to be oxidized through the pentose phosphate pathway (PPP) to increase NADPH generation. pfkA or pfkB single deletion and double-deletion strains were tested for their ability to produce lycopene. Since lycopene biosynthesis requires many NADPH, levels of lycopene were compared in a set of isogenic strains, with the pfkA single deletion strain showing the highest lycopene yield. Using another NADPH-requiring process, a one-step reduction reaction of 2-chloroacrylate to 2-chloropropionic acid by 2-haloacrylate reductase, the pfkA pfkB double-deletion strain showed the highest yield of 2-chloropropionic acid product. The combined effect of glucose-6-phosphate dehydrogenase overexpression or lactate dehydrogenase deletion with PFK deficiency on NADPH bioavailability was also studied. The results indicated that the flux distribution of fructose-6-phosphate between glycolysis and the pentose phosphate pathway determines the amount of NAPDH available for reductive biosynthesis.
copBerlin/Heidelberg
pubSpringer Berlin Heidelberg
doi10.1007/s00253-013-4859-0
pages6883-6893
date2013-08