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Bacillus amyloliquefaciens SC06 alleviates the oxidative stress of IPEC-1 via modulating Nrf2/Keap1 signaling pathway and decreasing ROS production

Oxidative stress (OS) plays a major role in the gastrointestinal disorders. Although probiotics were reported to repress OS, few researches compared the antioxidant ability of different Bacillus strains and deciphered the mechanisms. To select a Bacillus strain with higher antioxidant capacity, we u... Full description

Journal Title: Applied Microbiology and Biotechnology 2017, Vol.101(7), pp.3015-3026
Main Author: Wang, Yang
Other Authors: Wu, Yanping , Wang, Yibing , Fu, Aikun , Gong, Li , Li, Weifen , Li, Yali
Format: Electronic Article Electronic Article
Language: English
Subjects:
ROS
NOX
ID: ISSN: 0175-7598 ; E-ISSN: 1432-0614 ; DOI: 10.1007/s00253-016-8032-4
Link: http://dx.doi.org/10.1007/s00253-016-8032-4
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recordid: springer_jour10.1007/s00253-016-8032-4
title: Bacillus amyloliquefaciens SC06 alleviates the oxidative stress of IPEC-1 via modulating Nrf2/Keap1 signaling pathway and decreasing ROS production
format: Article
creator:
  • Wang, Yang
  • Wu, Yanping
  • Wang, Yibing
  • Fu, Aikun
  • Gong, Li
  • Li, Weifen
  • Li, Yali
subjects:
  • Bacillus
  • IPEC-1
  • Oxidative stress
  • Nrf2
  • ROS
  • NOX
ispartof: Applied Microbiology and Biotechnology, 2017, Vol.101(7), pp.3015-3026
description: Oxidative stress (OS) plays a major role in the gastrointestinal disorders. Although probiotics were reported to repress OS, few researches compared the antioxidant ability of different Bacillus strains and deciphered the mechanisms. To select a Bacillus strain with higher antioxidant capacity, we used H 2 O 2 to induce intestinal porcine epithelial cell 1 (IPEC-1) OS model. The most suitable H 2 O 2 concentration and incubation time were determined by the half lethal dose and methyl thiazolyl tetrazolium. Correlation analysis was performed to choose a sensitive indicator for OS. As for the comparison of Bacillus , cells were divided into control, Bacillus treatment, H 2 O 2 treatment, and Bacillus pre-protection + H 2 O 2 treatment. Bacillus were co-cultured with IPEC-1 for 3 h in Bacillus and Bacillus pre-protection + H 2 O 2 treatments. Then, based on OS model, 300 μmol/L H 2 O 2 was added into medium of H 2 O 2 and Bacillus pre-protection + H 2 O 2 treatments for another 12 h. Antioxidant and apoptosis gene expressions were detected to screen the target strain. Nuclear factor erythroid-derived 2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein1 (Keap1) pathway, reactive oxygen species (ROS) production, mitochondrial membrane potential (Δψm), apoptosis, and necrosis were analyzed. Results revealed that heme oxygenase-1 ( HO-1 ) gene expression had a positive correlation with H 2 O 2 induction. Moreover, Bacillus amyloliquefaciens SC06 (SC06)-meditated IPEC-1 showed the best antioxidant capacity though modulating Nrf2 phosphorylation. Δψm was elevated, while ROS generation was reduced with SC06 pre-protection, resulting in decreased apoptosis and necrosis. Altogether, HO-1 expression could be regarded as an OS indicator. The regulation of Nrf2/Keap1 pathway and ROS production by SC06 are involved in alleviating OS of IPEC-1.
language: eng
source:
identifier: ISSN: 0175-7598 ; E-ISSN: 1432-0614 ; DOI: 10.1007/s00253-016-8032-4
fulltext: fulltext
issn:
  • 1432-0614
  • 14320614
  • 0175-7598
  • 01757598
url: Link


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titleBacillus amyloliquefaciens SC06 alleviates the oxidative stress of IPEC-1 via modulating Nrf2/Keap1 signaling pathway and decreasing ROS production
creatorWang, Yang ; Wu, Yanping ; Wang, Yibing ; Fu, Aikun ; Gong, Li ; Li, Weifen ; Li, Yali
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subjectBacillus ; IPEC-1 ; Oxidative stress ; Nrf2 ; ROS ; NOX
descriptionOxidative stress (OS) plays a major role in the gastrointestinal disorders. Although probiotics were reported to repress OS, few researches compared the antioxidant ability of different Bacillus strains and deciphered the mechanisms. To select a Bacillus strain with higher antioxidant capacity, we used H 2 O 2 to induce intestinal porcine epithelial cell 1 (IPEC-1) OS model. The most suitable H 2 O 2 concentration and incubation time were determined by the half lethal dose and methyl thiazolyl tetrazolium. Correlation analysis was performed to choose a sensitive indicator for OS. As for the comparison of Bacillus , cells were divided into control, Bacillus treatment, H 2 O 2 treatment, and Bacillus pre-protection + H 2 O 2 treatment. Bacillus were co-cultured with IPEC-1 for 3 h in Bacillus and Bacillus pre-protection + H 2 O 2 treatments. Then, based on OS model, 300 μmol/L H 2 O 2 was added into medium of H 2 O 2 and Bacillus pre-protection + H 2 O 2 treatments for another 12 h. Antioxidant and apoptosis gene expressions were detected to screen the target strain. Nuclear factor erythroid-derived 2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein1 (Keap1) pathway, reactive oxygen species (ROS) production, mitochondrial membrane potential (Δψm), apoptosis, and necrosis were analyzed. Results revealed that heme oxygenase-1 ( HO-1 ) gene expression had a positive correlation with H 2 O 2 induction. Moreover, Bacillus amyloliquefaciens SC06 (SC06)-meditated IPEC-1 showed the best antioxidant capacity though modulating Nrf2 phosphorylation. Δψm was elevated, while ROS generation was reduced with SC06 pre-protection, resulting in decreased apoptosis and necrosis. Altogether, HO-1 expression could be regarded as an OS indicator. The regulation of Nrf2/Keap1 pathway and ROS production by SC06 are involved in alleviating OS of IPEC-1.
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titleBacillus amyloliquefaciens SC06 alleviates the oxidative stress of IPEC-1 via modulating Nrf2/Keap1 signaling pathway and decreasing ROS production
descriptionOxidative stress (OS) plays a major role in the gastrointestinal disorders. Although probiotics were reported to repress OS, few researches compared the antioxidant ability of different Bacillus strains and deciphered the mechanisms. To select a Bacillus strain with higher antioxidant capacity, we used H 2 O 2 to induce intestinal porcine epithelial cell 1 (IPEC-1) OS model. The most suitable H 2 O 2 concentration and incubation time were determined by the half lethal dose and methyl thiazolyl tetrazolium. Correlation analysis was performed to choose a sensitive indicator for OS. As for the comparison of Bacillus , cells were divided into control, Bacillus treatment, H 2 O 2 treatment, and Bacillus pre-protection + H 2 O 2 treatment. Bacillus were co-cultured with IPEC-1 for 3 h in Bacillus and Bacillus pre-protection + H 2 O 2 treatments. Then, based on OS model, 300 μmol/L H 2 O 2 was added into medium of H 2 O 2 and Bacillus pre-protection + H 2 O 2 treatments for another 12 h. Antioxidant and apoptosis gene expressions were detected to screen the target strain. Nuclear factor erythroid-derived 2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein1 (Keap1) pathway, reactive oxygen species (ROS) production, mitochondrial membrane potential (Δψm), apoptosis, and necrosis were analyzed. Results revealed that heme oxygenase-1 ( HO-1 ) gene expression had a positive correlation with H 2 O 2 induction. Moreover, Bacillus amyloliquefaciens SC06 (SC06)-meditated IPEC-1 showed the best antioxidant capacity though modulating Nrf2 phosphorylation. Δψm was elevated, while ROS generation was reduced with SC06 pre-protection, resulting in decreased apoptosis and necrosis. Altogether, HO-1 expression could be regarded as an OS indicator. The regulation of Nrf2/Keap1 pathway and ROS production by SC06 are involved in alleviating OS of IPEC-1.
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abstractOxidative stress (OS) plays a major role in the gastrointestinal disorders. Although probiotics were reported to repress OS, few researches compared the antioxidant ability of different Bacillus strains and deciphered the mechanisms. To select a Bacillus strain with higher antioxidant capacity, we used H 2 O 2 to induce intestinal porcine epithelial cell 1 (IPEC-1) OS model. The most suitable H 2 O 2 concentration and incubation time were determined by the half lethal dose and methyl thiazolyl tetrazolium. Correlation analysis was performed to choose a sensitive indicator for OS. As for the comparison of Bacillus , cells were divided into control, Bacillus treatment, H 2 O 2 treatment, and Bacillus pre-protection + H 2 O 2 treatment. Bacillus were co-cultured with IPEC-1 for 3 h in Bacillus and Bacillus pre-protection + H 2 O 2 treatments. Then, based on OS model, 300 μmol/L H 2 O 2 was added into medium of H 2 O 2 and Bacillus pre-protection + H 2 O 2 treatments for another 12 h. Antioxidant and apoptosis gene expressions were detected to screen the target strain. Nuclear factor erythroid-derived 2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein1 (Keap1) pathway, reactive oxygen species (ROS) production, mitochondrial membrane potential (Δψm), apoptosis, and necrosis were analyzed. Results revealed that heme oxygenase-1 ( HO-1 ) gene expression had a positive correlation with H 2 O 2 induction. Moreover, Bacillus amyloliquefaciens SC06 (SC06)-meditated IPEC-1 showed the best antioxidant capacity though modulating Nrf2 phosphorylation. Δψm was elevated, while ROS generation was reduced with SC06 pre-protection, resulting in decreased apoptosis and necrosis. Altogether, HO-1 expression could be regarded as an OS indicator. The regulation of Nrf2/Keap1 pathway and ROS production by SC06 are involved in alleviating OS of IPEC-1.
copBerlin/Heidelberg
pubSpringer Berlin Heidelberg
doi10.1007/s00253-016-8032-4
pages3015-3026
date2017-04