schliessen

Filtern

 

Bibliotheken

Suppression of in vivo tumorigenicity of rat hepatoma cell line KDH-8 cells by soluble TGF-β receptor type II

We have previously reported that transforming growth factor beta (TGF-β) produced by rat hepatoma cell line KDH-8 cells suppressed the interleukin-2 (IL-2) production of T cells and the tumoricidal activity of macrophages in KDH-8 tumor-bearing rats and that the inhibition of TGF-β production by low... Full description

Journal Title: Cancer Immunology Immunotherapy, 2002, Vol.51(7), pp.381-388
Main Author: Zhao, Wenli
Other Authors: Kobayashi, Masonobu , Ding, Wei , Yuan, Lan , Seth, Prem , Cornain, Santoso , Wang, Jingxin , Okada, Futoshi , Hosokawa, Masuo
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0340-7004 ; E-ISSN: 1432-0851 ; DOI: 10.1007/s00262-002-0290-6
Link: http://dx.doi.org/10.1007/s00262-002-0290-6
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: springer_jour10.1007/s00262-002-0290-6
title: Suppression of in vivo tumorigenicity of rat hepatoma cell line KDH-8 cells by soluble TGF-β receptor type II
format: Article
creator:
  • Zhao, Wenli
  • Kobayashi, Masonobu
  • Ding, Wei
  • Yuan, Lan
  • Seth, Prem
  • Cornain, Santoso
  • Wang, Jingxin
  • Okada, Futoshi
  • Hosokawa, Masuo
subjects:
  • Immunorestoration Rat hepatocarcinoma TGF-β TGF-β receptor type II
ispartof: Cancer Immunology, Immunotherapy, 2002, Vol.51(7), pp.381-388
description: We have previously reported that transforming growth factor beta (TGF-β) produced by rat hepatoma cell line KDH-8 cells suppressed the interleukin-2 (IL-2) production of T cells and the tumoricidal activity of macrophages in KDH-8 tumor-bearing rats and that the inhibition of TGF-β production by low-dose bleomycin restored these activities significantly. In this study, we established three transfectant clones with stable expression of soluble TGF-β receptor type II (sTRII), namely KT1, KT2 and KT3, and one with an empty vector used as control vector (KV), and then investigated the effects of sTRII on the tumorigenicity of KDH-8 cells and immune responses in syngeneic Wistar King Aptekman/Hok (WKAH) rats. We found that sTRII expressed in sTRII transfectants could abolish growth inhibition of Mv1Lu cells by TGF-β1 produced by the cells themselves, and that tumor growth of KT2 and KT3 clones in vivo was suppressed significantly compared with that of parent, KV and KT1 clones. Furthermore, we demonstrated that IL-2 production of splenocytes and IL12p40 mRNA expression in tumor tissues were restored in rats inoculated with KT2 and KT3 clones, whereas such restoration was not observed in rats inoculated with parent, KV and KT1 clones. Combined with a low expression of sTRII in KT1 tumor tissues, these results suggest that sTRII may to some extent be able to abolish the tumor-promoting activity of TGF-β, and imply that sTRII might have a therapeutic effect on TGF-β-producing tumors.
language: eng
source:
identifier: ISSN: 0340-7004 ; E-ISSN: 1432-0851 ; DOI: 10.1007/s00262-002-0290-6
fulltext: fulltext
issn:
  • 1432-0851
  • 14320851
  • 0340-7004
  • 03407004
url: Link


@attributes
ID172394979
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.1007/s00262-002-0290-6
sourceidspringer_jour
recordidTN_springer_jour10.1007/s00262-002-0290-6
sourcesystemPC
pqid18771358
display
typearticle
titleSuppression of in vivo tumorigenicity of rat hepatoma cell line KDH-8 cells by soluble TGF-β receptor type II
creatorZhao, Wenli ; Kobayashi, Masonobu ; Ding, Wei ; Yuan, Lan ; Seth, Prem ; Cornain, Santoso ; Wang, Jingxin ; Okada, Futoshi ; Hosokawa, Masuo
ispartofCancer Immunology, Immunotherapy, 2002, Vol.51(7), pp.381-388
identifier
subjectImmunorestoration Rat hepatocarcinoma TGF-β TGF-β receptor type II
descriptionWe have previously reported that transforming growth factor beta (TGF-β) produced by rat hepatoma cell line KDH-8 cells suppressed the interleukin-2 (IL-2) production of T cells and the tumoricidal activity of macrophages in KDH-8 tumor-bearing rats and that the inhibition of TGF-β production by low-dose bleomycin restored these activities significantly. In this study, we established three transfectant clones with stable expression of soluble TGF-β receptor type II (sTRII), namely KT1, KT2 and KT3, and one with an empty vector used as control vector (KV), and then investigated the effects of sTRII on the tumorigenicity of KDH-8 cells and immune responses in syngeneic Wistar King Aptekman/Hok (WKAH) rats. We found that sTRII expressed in sTRII transfectants could abolish growth inhibition of Mv1Lu cells by TGF-β1 produced by the cells themselves, and that tumor growth of KT2 and KT3 clones in vivo was suppressed significantly compared with that of parent, KV and KT1 clones. Furthermore, we demonstrated that IL-2 production of splenocytes and IL12p40 mRNA expression in tumor tissues were restored in rats inoculated with KT2 and KT3 clones, whereas such restoration was not observed in rats inoculated with parent, KV and KT1 clones. Combined with a low expression of sTRII in KT1 tumor tissues, these results suggest that sTRII may to some extent be able to abolish the tumor-promoting activity of TGF-β, and imply that sTRII might have a therapeutic effect on TGF-β-producing tumors.
languageeng
source
version6
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://dx.doi.org/10.1007/s00262-002-0290-6$$EView_full_text_in_Springer_(Subscribers_only)
search
creatorcontrib
0Zhao, Wenli
1Kobayashi, Masonobu
2Ding, Wei
3Yuan, Lan
4Seth, Prem
5Cornain, Santoso
6Wang, Jingxin
7Okada, Futoshi
8Hosokawa, Masuo
titleSuppression of in vivo tumorigenicity of rat hepatoma cell line KDH-8 cells by soluble TGF-β receptor type II
descriptionWe have previously reported that transforming growth factor beta (TGF-β) produced by rat hepatoma cell line KDH-8 cells suppressed the interleukin-2 (IL-2) production of T cells and the tumoricidal activity of macrophages in KDH-8 tumor-bearing rats and that the inhibition of TGF-β production by low-dose bleomycin restored these activities significantly. In this study, we established three transfectant clones with stable expression of soluble TGF-β receptor type II (sTRII), namely KT1, KT2 and KT3, and one with an empty vector used as control vector (KV), and then investigated the effects of sTRII on the tumorigenicity of KDH-8 cells and immune responses in syngeneic Wistar King Aptekman/Hok (WKAH) rats. We found that sTRII expressed in sTRII transfectants could abolish growth inhibition of Mv1Lu cells by TGF-β1 produced by the cells themselves, and that tumor growth of KT2 and KT3 clones in vivo was suppressed significantly compared with that of parent, KV and KT1 clones. Furthermore, we demonstrated that IL-2 production of splenocytes and IL12p40 mRNA expression in tumor tissues were restored in rats inoculated with KT2 and KT3 clones, whereas such restoration was not observed in rats inoculated with parent, KV and KT1 clones. Combined with a low expression of sTRII in KT1 tumor tissues, these results suggest that sTRII may to some extent be able to abolish the tumor-promoting activity of TGF-β, and imply that sTRII might have a therapeutic effect on TGF-β-producing tumors.
subjectImmunorestoration Rat hepatocarcinoma TGF-β TGF-β receptor type II
general
010.1007/s00262-002-0290-6
1English
2Springer Science & Business Media B.V.
3SpringerLink
sourceidspringer_jour
recordidspringer_jour10.1007/s00262-002-0290-6
issn
01432-0851
114320851
20340-7004
303407004
rsrctypearticle
creationdate2002
addtitle
0Cancer Immunology, Immunotherapy
1Cancer Immunol Immunother
searchscopespringer_journals_complete
scopespringer_journals_complete
lsr30VSR-Enriched:[pages, pqid]
sort
titleSuppression of in vivo tumorigenicity of rat hepatoma cell line KDH-8 cells by soluble TGF-β receptor type II
authorZhao, Wenli ; Kobayashi, Masonobu ; Ding, Wei ; Yuan, Lan ; Seth, Prem ; Cornain, Santoso ; Wang, Jingxin ; Okada, Futoshi ; Hosokawa, Masuo
creationdate20020900
facets
frbrgroupid7786604771289721486
frbrtype5
languageeng
creationdate2002
topicImmunorestoration Rat Hepatocarcinoma Tgf-Β Tgf-Β Receptor Type Ii
collectionSpringerLink
prefilterarticles
rsrctypearticles
creatorcontrib
0Zhao, Wenli
1Kobayashi, Masonobu
2Ding, Wei
3Yuan, Lan
4Seth, Prem
5Cornain, Santoso
6Wang, Jingxin
7Okada, Futoshi
8Hosokawa, Masuo
jtitleCancer Immunology, Immunotherapy
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Zhao
1Kobayashi
2Ding
3Yuan
4Seth
5Cornain
6Wang
7Okada
8Hosokawa
aufirst
0Wenli
1Masonobu
2Wei
3Lan
4Prem
5Santoso
6Jingxin
7Futoshi
8Masuo
au
0Zhao, Wenli
1Kobayashi, Masonobu
2Ding, Wei
3Yuan, Lan
4Seth, Prem
5Cornain, Santoso
6Wang, Jingxin
7Okada, Futoshi
8Hosokawa, Masuo
atitleSuppression of in vivo tumorigenicity of rat hepatoma cell line KDH-8 cells by soluble TGF-β receptor type II
jtitleCancer Immunology, Immunotherapy
stitleCancer Immunol Immunother
risdate200209
volume51
issue7
spage381
epage388
issn0340-7004
eissn1432-0851
genrearticle
ristypeJOUR
abstractWe have previously reported that transforming growth factor beta (TGF-β) produced by rat hepatoma cell line KDH-8 cells suppressed the interleukin-2 (IL-2) production of T cells and the tumoricidal activity of macrophages in KDH-8 tumor-bearing rats and that the inhibition of TGF-β production by low-dose bleomycin restored these activities significantly. In this study, we established three transfectant clones with stable expression of soluble TGF-β receptor type II (sTRII), namely KT1, KT2 and KT3, and one with an empty vector used as control vector (KV), and then investigated the effects of sTRII on the tumorigenicity of KDH-8 cells and immune responses in syngeneic Wistar King Aptekman/Hok (WKAH) rats. We found that sTRII expressed in sTRII transfectants could abolish growth inhibition of Mv1Lu cells by TGF-β1 produced by the cells themselves, and that tumor growth of KT2 and KT3 clones in vivo was suppressed significantly compared with that of parent, KV and KT1 clones. Furthermore, we demonstrated that IL-2 production of splenocytes and IL12p40 mRNA expression in tumor tissues were restored in rats inoculated with KT2 and KT3 clones, whereas such restoration was not observed in rats inoculated with parent, KV and KT1 clones. Combined with a low expression of sTRII in KT1 tumor tissues, these results suggest that sTRII may to some extent be able to abolish the tumor-promoting activity of TGF-β, and imply that sTRII might have a therapeutic effect on TGF-β-producing tumors.
copBerlin/Heidelberg
pubSpringer-Verlag
doi10.1007/s00262-002-0290-6
pages381-388
date2002-09