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Correlation of SATB1 overexpression with the progression of human rectal cancer

Byline: Wen-Jian Meng (1,2), Hui Yan (1,2), Bin Zhou (2), Wei Zhang (1,2), Xiang-Heng Kong (1,2), Rong Wang (2), Lan Zhan (2), Yuan Li (2,3,4), Zong-Guang Zhou (1,2,4), Xiao-Feng Sun (2,5) Keywords: Special AT-rich sequence-binding protein 1; Human rectal cancer; Quantitative real-time PCR; Immunohi... Full description

Journal Title: International Journal of Colorectal Disease 2012, Vol.27(2), pp.143-150
Main Author: Meng, Wen-Jian
Other Authors: Yan, Hui , Zhou, Bin , Zhang, Wei , Kong, Xiang-Heng , Wang, Rong , Zhan, Lan , Li, Yuan , Zhou, Zong-Guang , Sun, Xiao-Feng
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0179-1958 ; E-ISSN: 1432-1262 ; DOI: 10.1007/s00384-011-1302-9
Link: http://dx.doi.org/10.1007/s00384-011-1302-9
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recordid: springer_jour10.1007/s00384-011-1302-9
title: Correlation of SATB1 overexpression with the progression of human rectal cancer
format: Article
creator:
  • Meng, Wen-Jian
  • Yan, Hui
  • Zhou, Bin
  • Zhang, Wei
  • Kong, Xiang-Heng
  • Wang, Rong
  • Zhan, Lan
  • Li, Yuan
  • Zhou, Zong-Guang
  • Sun, Xiao-Feng
subjects:
  • Special AT-rich sequence-binding protein 1
  • Human rectal cancer
  • Quantitative real-time PCR
  • Immunohistochemistry
  • KM12C cell lines
ispartof: International Journal of Colorectal Disease, 2012, Vol.27(2), pp.143-150
description: Byline: Wen-Jian Meng (1,2), Hui Yan (1,2), Bin Zhou (2), Wei Zhang (1,2), Xiang-Heng Kong (1,2), Rong Wang (2), Lan Zhan (2), Yuan Li (2,3,4), Zong-Guang Zhou (1,2,4), Xiao-Feng Sun (2,5) Keywords: Special AT-rich sequence-binding protein 1; Human rectal cancer; Quantitative real-time PCR; Immunohistochemistry; KM12C cell lines Abstract: Background and aims To date, the association between special AT-rich sequence-binding protein 1 (SATB1) and colorectal cancer (CRC) has not been reported. This study was aimed at investigating the expression and potential role of SATB1 in human rectal cancers. Methods Ninety-three paired samples of rectal cancer and distant normal rectal tissue were analyzed by quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC), and the correlations between SATB1 expression and clinicopathological parameters were evaluated. The expression profiles of SATB1 were also investigated in a panel of five human colon carcinoma cell lines. Results The general level of SATB1 mRNA in rectal cancer tissues was statistically significantly higher than that in normal mucosa (P=0.043). The rate of positive SATB1 protein expression in rectal cancers (44.1%) was significantly higher than that in normal tissues (25.8%) by IHC analysis (P=0.009). Overexpression of SATB1 mRNA was more predominant in patients with earlier onset of rectal cancer (P=0.033). SATB1 expression correlated with invasive depth and tumor node metastasis (TNM) stage at both protein and mRNA levels (P
language: eng
source:
identifier: ISSN: 0179-1958 ; E-ISSN: 1432-1262 ; DOI: 10.1007/s00384-011-1302-9
fulltext: fulltext
issn:
  • 1432-1262
  • 14321262
  • 0179-1958
  • 01791958
url: Link


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titleCorrelation of SATB1 overexpression with the progression of human rectal cancer
creatorMeng, Wen-Jian ; Yan, Hui ; Zhou, Bin ; Zhang, Wei ; Kong, Xiang-Heng ; Wang, Rong ; Zhan, Lan ; Li, Yuan ; Zhou, Zong-Guang ; Sun, Xiao-Feng
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subjectSpecial AT-rich sequence-binding protein 1 ; Human rectal cancer ; Quantitative real-time PCR ; Immunohistochemistry ; KM12C cell lines
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descriptionByline: Wen-Jian Meng (1,2), Hui Yan (1,2), Bin Zhou (2), Wei Zhang (1,2), Xiang-Heng Kong (1,2), Rong Wang (2), Lan Zhan (2), Yuan Li (2,3,4), Zong-Guang Zhou (1,2,4), Xiao-Feng Sun (2,5) Keywords: Special AT-rich sequence-binding protein 1; Human rectal cancer; Quantitative real-time PCR; Immunohistochemistry; KM12C cell lines Abstract: Background and aims To date, the association between special AT-rich sequence-binding protein 1 (SATB1) and colorectal cancer (CRC) has not been reported. This study was aimed at investigating the expression and potential role of SATB1 in human rectal cancers. Methods Ninety-three paired samples of rectal cancer and distant normal rectal tissue were analyzed by quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC), and the correlations between SATB1 expression and clinicopathological parameters were evaluated. The expression profiles of SATB1 were also investigated in a panel of five human colon carcinoma cell lines. Results The general level of SATB1 mRNA in rectal cancer tissues was statistically significantly higher than that in normal mucosa (P=0.043). The rate of positive SATB1 protein expression in rectal cancers (44.1%) was significantly higher than that in normal tissues (25.8%) by IHC analysis (P=0.009). Overexpression of SATB1 mRNA was more predominant in patients with earlier onset of rectal cancer (P=0.033). SATB1 expression correlated with invasive depth and tumor node metastasis (TNM) stage at both protein and mRNA levels (P<0.05). Furthermore, SATB1 expression in the poorly metastatic KM12C cells was significantly lower than the highly metastatic KM12SM and KM12L4A cells and higher than the HCT116 and SW480 cells (P=0.001). These results were further confirmed by Western blotting. Conclusion Our results indicate that SATB1 may play an important role in the progression of human rectal cancer, which represents a possible new mechanism underlying CRC. Author Affiliation: (1) Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, 37 Guo Xue Xiang, Chengdu, 610041, China (2) Institute of Digestive Surgery and Organ Microcirculation, West China Hospital, Sichuan University, 1 Keyuan Road 4, Chengdu, 610041, China (3) Department of Pediatric Surgery, West China Hospital, Sichuan University, 37 Guo Xue Xiang, Chengdu, 610041, China (4) National Key Laboratory of Biotherapy of West China Hospital, Sichuan University, 1 Keyuan Road 4, Chengdu, 610041, China (5) Department of Oncology, Institute of Clinical and Experimental Medicine, Linkoping University, S-581 85, Linkoping, Sweden Article History: Registration Date: 03/08/2011 Accepted Date: 03/08/2011 Online Date: 26/08/2011 Article note: Electronic supplementary material The online version of this article (doi: 10.1007/s00384-011-1302-9) contains supplementary material, which is available to authorized users.
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