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Immunochromatographic lateral flow strip for on-site detection of bisphenol A

We have developed a lateral flow assay (LFA) for the detection of bisphenol A (BPA) in water samples. Antibody against BPA was labeled with gold nanoparticles, and these conjugates were used as the recognition probes for the construction of an LFA strip. The diameter of the gold nanoparticles, the a... Full description

Journal Title: Microchimica Acta 2013, Vol.180(3), pp.279-285
Main Author: Mei, Zhanlong
Other Authors: Deng, Yi , Chu, Huaqin , Xue, Feng , Zhong, Youhao , Wu, Jingjing , Yang, Huan , Wang, Zhichao , Zheng, Lei , Chen, Wei
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0026-3672 ; E-ISSN: 1436-5073 ; DOI: 10.1007/s00604-012-0930-2
Link: http://dx.doi.org/10.1007/s00604-012-0930-2
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recordid: springer_jour10.1007/s00604-012-0930-2
title: Immunochromatographic lateral flow strip for on-site detection of bisphenol A
format: Article
creator:
  • Mei, Zhanlong
  • Deng, Yi
  • Chu, Huaqin
  • Xue, Feng
  • Zhong, Youhao
  • Wu, Jingjing
  • Yang, Huan
  • Wang, Zhichao
  • Zheng, Lei
  • Chen, Wei
subjects:
  • Lateral flow strip
  • Bisphenol A
  • On-site detection
  • Food safety
ispartof: Microchimica Acta, 2013, Vol.180(3), pp.279-285
description: We have developed a lateral flow assay (LFA) for the detection of bisphenol A (BPA) in water samples. Antibody against BPA was labeled with gold nanoparticles, and these conjugates were used as the recognition probes for the construction of an LFA strip. The diameter of the gold nanoparticles, the amount of antibody, the pH of the buffer, and the categories of the conjugation pad were optimized. The resulting method has a (visual) detection limit of 5 ppb, and of 0.92 ppb if used in combination with professional software. This LFA displays excellent specificity and was applied to spiked water samples with satisfactory results. Figure Bisphenol A in water samples could be rapid and sensitively screened by the immunochramatographic lateral flow strip in less than 15 min. The limit of dectection was as low as 5 ppb and 0.92 ppb by naked-eye observation and software analysis, respectively, which meet the requirements of on-site and rapid detection of BPA in water samples.
language: eng
source:
identifier: ISSN: 0026-3672 ; E-ISSN: 1436-5073 ; DOI: 10.1007/s00604-012-0930-2
fulltext: fulltext
issn:
  • 1436-5073
  • 14365073
  • 0026-3672
  • 00263672
url: Link


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titleImmunochromatographic lateral flow strip for on-site detection of bisphenol A
creatorMei, Zhanlong ; Deng, Yi ; Chu, Huaqin ; Xue, Feng ; Zhong, Youhao ; Wu, Jingjing ; Yang, Huan ; Wang, Zhichao ; Zheng, Lei ; Chen, Wei
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subjectLateral flow strip ; Bisphenol A ; On-site detection ; Food safety
descriptionWe have developed a lateral flow assay (LFA) for the detection of bisphenol A (BPA) in water samples. Antibody against BPA was labeled with gold nanoparticles, and these conjugates were used as the recognition probes for the construction of an LFA strip. The diameter of the gold nanoparticles, the amount of antibody, the pH of the buffer, and the categories of the conjugation pad were optimized. The resulting method has a (visual) detection limit of 5 ppb, and of 0.92 ppb if used in combination with professional software. This LFA displays excellent specificity and was applied to spiked water samples with satisfactory results. Figure Bisphenol A in water samples could be rapid and sensitively screened by the immunochramatographic lateral flow strip in less than 15 min. The limit of dectection was as low as 5 ppb and 0.92 ppb by naked-eye observation and software analysis, respectively, which meet the requirements of on-site and rapid detection of BPA in water samples.
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1Figure Bisphenol A in water samples could be rapid and sensitively screened by the immunochramatographic lateral flow strip in less than 15 min. The limit of dectection was as low as 5 ppb and 0.92 ppb by naked-eye observation and software analysis, respectively, which meet the requirements of on-site and rapid detection of BPA in water samples.
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titleImmunochromatographic lateral flow strip for on-site detection of bisphenol A
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abstractWe have developed a lateral flow assay (LFA) for the detection of bisphenol A (BPA) in water samples. Antibody against BPA was labeled with gold nanoparticles, and these conjugates were used as the recognition probes for the construction of an LFA strip. The diameter of the gold nanoparticles, the amount of antibody, the pH of the buffer, and the categories of the conjugation pad were optimized. The resulting method has a (visual) detection limit of 5 ppb, and of 0.92 ppb if used in combination with professional software. This LFA displays excellent specificity and was applied to spiked water samples with satisfactory results. Figure Bisphenol A in water samples could be rapid and sensitively screened by the immunochramatographic lateral flow strip in less than 15 min. The limit of dectection was as low as 5 ppb and 0.92 ppb by naked-eye observation and software analysis, respectively, which meet the requirements of on-site and rapid detection of BPA in water samples.
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pubSpringer Vienna
doi10.1007/s00604-012-0930-2
pages279-285
date2013-02