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Recent trends in SELEX technique and its application to food safety monitoring

The method referred to as “systemic evolution of ligands by exponential enrichment” (SELEX) was introduced in 1990 and ever since has become an important tool for the identification and screening of aptamers. Such nucleic acids can recognize and bind to their corresponding targets (analytes) with hi... Full description

Journal Title: Microchimica Acta 2014, Vol.181(5), pp.479-491
Main Author: Wu, Jingjing
Other Authors: Zhu, Yingyue , Xue, Feng , Mei, Zhanlong , Yao, Li , Wang, Xin , Zheng, Lei , Liu, Jian , Liu, Guodong , Peng, Chifang , Chen, Wei
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0026-3672 ; E-ISSN: 1436-5073 ; DOI: 10.1007/s00604-013-1156-7
Link: http://dx.doi.org/10.1007/s00604-013-1156-7
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recordid: springer_jour10.1007/s00604-013-1156-7
title: Recent trends in SELEX technique and its application to food safety monitoring
format: Article
creator:
  • Wu, Jingjing
  • Zhu, Yingyue
  • Xue, Feng
  • Mei, Zhanlong
  • Yao, Li
  • Wang, Xin
  • Zheng, Lei
  • Liu, Jian
  • Liu, Guodong
  • Peng, Chifang
  • Chen, Wei
subjects:
  • SELEX
  • Aptamer
  • Food safety
  • Rapid detection
  • Biosensor
ispartof: Microchimica Acta, 2014, Vol.181(5), pp.479-491
description: The method referred to as “systemic evolution of ligands by exponential enrichment” (SELEX) was introduced in 1990 and ever since has become an important tool for the identification and screening of aptamers. Such nucleic acids can recognize and bind to their corresponding targets (analytes) with high selectivity and affinity, and aptamers therefore have become attractive alternatives to traditional antibodies not the least because they are much more stable. Meanwhile, they have found numerous applications in different fields including food quality and safety monitoring. This review first gives an introduction into the selection process and to the evolution of SELEX, then covers applications of aptamers in the surveillance of food safety (with subsections on absorptiometric, electrochemical, fluorescent and other methods), and then gives conclusions and perspectives. The SELEX method excels by its features of in vitro, high throughput and ease of operation. This review contains 86 references. Figure Aptamers, novel recognition probes screened with SELEX, have been adopted as substitution to antibody in various fields and also widely applied in food safety monitoring.
language: eng
source:
identifier: ISSN: 0026-3672 ; E-ISSN: 1436-5073 ; DOI: 10.1007/s00604-013-1156-7
fulltext: fulltext
issn:
  • 1436-5073
  • 14365073
  • 0026-3672
  • 00263672
url: Link


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titleRecent trends in SELEX technique and its application to food safety monitoring
creatorWu, Jingjing ; Zhu, Yingyue ; Xue, Feng ; Mei, Zhanlong ; Yao, Li ; Wang, Xin ; Zheng, Lei ; Liu, Jian ; Liu, Guodong ; Peng, Chifang ; Chen, Wei
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subjectSELEX ; Aptamer ; Food safety ; Rapid detection ; Biosensor
descriptionThe method referred to as “systemic evolution of ligands by exponential enrichment” (SELEX) was introduced in 1990 and ever since has become an important tool for the identification and screening of aptamers. Such nucleic acids can recognize and bind to their corresponding targets (analytes) with high selectivity and affinity, and aptamers therefore have become attractive alternatives to traditional antibodies not the least because they are much more stable. Meanwhile, they have found numerous applications in different fields including food quality and safety monitoring. This review first gives an introduction into the selection process and to the evolution of SELEX, then covers applications of aptamers in the surveillance of food safety (with subsections on absorptiometric, electrochemical, fluorescent and other methods), and then gives conclusions and perspectives. The SELEX method excels by its features of in vitro, high throughput and ease of operation. This review contains 86 references. Figure Aptamers, novel recognition probes screened with SELEX, have been adopted as substitution to antibody in various fields and also widely applied in food safety monitoring.
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abstractThe method referred to as “systemic evolution of ligands by exponential enrichment” (SELEX) was introduced in 1990 and ever since has become an important tool for the identification and screening of aptamers. Such nucleic acids can recognize and bind to their corresponding targets (analytes) with high selectivity and affinity, and aptamers therefore have become attractive alternatives to traditional antibodies not the least because they are much more stable. Meanwhile, they have found numerous applications in different fields including food quality and safety monitoring. This review first gives an introduction into the selection process and to the evolution of SELEX, then covers applications of aptamers in the surveillance of food safety (with subsections on absorptiometric, electrochemical, fluorescent and other methods), and then gives conclusions and perspectives. The SELEX method excels by its features of in vitro, high throughput and ease of operation. This review contains 86 references. Figure Aptamers, novel recognition probes screened with SELEX, have been adopted as substitution to antibody in various fields and also widely applied in food safety monitoring.
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pubSpringer Vienna
doi10.1007/s00604-013-1156-7
pages479-491
date2014-04