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Magnetic microparticle-based SELEX process for the identification of highly specific aptamers of heart marker--brain natriuretic peptide

The brain natriuretic peptide (BNP) is known to be an effective indicator of heart failure. It has been widely adopted as a parameter for the evaluation of heart function of cardiovascular and cerebrovascular diseases (CVDs). Current immune-recognition based methods for the detection of BNP are limi... Full description

Journal Title: Microchimica Acta 2015, Vol.182(1), pp.331-339
Main Author: Wang, Ying
Other Authors: Wu, Jingjing , Chen, Yinji , Xue, Feng , Teng, Jun , Cao, Jinxuan , Lu, Chunxia , Chen, Wei
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0026-3672 ; E-ISSN: 1436-5073 ; DOI: 10.1007/s00604-014-1338-y
Link: http://dx.doi.org/10.1007/s00604-014-1338-y
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recordid: springer_jour10.1007/s00604-014-1338-y
title: Magnetic microparticle-based SELEX process for the identification of highly specific aptamers of heart marker--brain natriuretic peptide
format: Article
creator:
  • Wang, Ying
  • Wu, Jingjing
  • Chen, Yinji
  • Xue, Feng
  • Teng, Jun
  • Cao, Jinxuan
  • Lu, Chunxia
  • Chen, Wei
subjects:
  • SELEX
  • Magnetic microparticle, Aptamers, Brain natriuretic peptide (BNP), Rapid and early diagnosis
ispartof: Microchimica Acta, 2015, Vol.182(1), pp.331-339
description: The brain natriuretic peptide (BNP) is known to be an effective indicator of heart failure. It has been widely adopted as a parameter for the evaluation of heart function of cardiovascular and cerebrovascular diseases (CVDs). Current immune-recognition based methods for the detection of BNP are limited, to a certain extent, by the poor stability of the antibody and by high costs. The availability of an aptamer specific for BNP would greatly assist in the rapid and early diagnosis of CVDs. In order to screen for such an aptamer by the SELEX method, we have used magnetic microparticles (m-MPs) as the separation substrate for immobilization of target BNP. The use of m-MPs for rapid separation of combined aptamers enables bound oligonucleotides to be separated directly, quickly, and with high efficiency. After 14 rounds of selection, a panel of six aptamers against BNP was identified. Their dissociation constants range from 12.5 to 139 nM. The classical technique for conjugation of a target to m-MPs is known to be applicable to various fields, and we conclude that this m-MP-based SELEX process provides a general strategy for screening of specific aptamers against various analytes. Graphical Abstract Rapid selection of aptamer to brain natriuretic peptide (BNP) with high affinity and specificity for rapid detection and daily monitoring of heart functions with the magnetic microparticles-based screening platform.
language: eng
source:
identifier: ISSN: 0026-3672 ; E-ISSN: 1436-5073 ; DOI: 10.1007/s00604-014-1338-y
fulltext: fulltext
issn:
  • 1436-5073
  • 14365073
  • 0026-3672
  • 00263672
url: Link


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titleMagnetic microparticle-based SELEX process for the identification of highly specific aptamers of heart marker--brain natriuretic peptide
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descriptionThe brain natriuretic peptide (BNP) is known to be an effective indicator of heart failure. It has been widely adopted as a parameter for the evaluation of heart function of cardiovascular and cerebrovascular diseases (CVDs). Current immune-recognition based methods for the detection of BNP are limited, to a certain extent, by the poor stability of the antibody and by high costs. The availability of an aptamer specific for BNP would greatly assist in the rapid and early diagnosis of CVDs. In order to screen for such an aptamer by the SELEX method, we have used magnetic microparticles (m-MPs) as the separation substrate for immobilization of target BNP. The use of m-MPs for rapid separation of combined aptamers enables bound oligonucleotides to be separated directly, quickly, and with high efficiency. After 14 rounds of selection, a panel of six aptamers against BNP was identified. Their dissociation constants range from 12.5 to 139 nM. The classical technique for conjugation of a target to m-MPs is known to be applicable to various fields, and we conclude that this m-MP-based SELEX process provides a general strategy for screening of specific aptamers against various analytes. Graphical Abstract Rapid selection of aptamer to brain natriuretic peptide (BNP) with high affinity and specificity for rapid detection and daily monitoring of heart functions with the magnetic microparticles-based screening platform.
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abstractThe brain natriuretic peptide (BNP) is known to be an effective indicator of heart failure. It has been widely adopted as a parameter for the evaluation of heart function of cardiovascular and cerebrovascular diseases (CVDs). Current immune-recognition based methods for the detection of BNP are limited, to a certain extent, by the poor stability of the antibody and by high costs. The availability of an aptamer specific for BNP would greatly assist in the rapid and early diagnosis of CVDs. In order to screen for such an aptamer by the SELEX method, we have used magnetic microparticles (m-MPs) as the separation substrate for immobilization of target BNP. The use of m-MPs for rapid separation of combined aptamers enables bound oligonucleotides to be separated directly, quickly, and with high efficiency. After 14 rounds of selection, a panel of six aptamers against BNP was identified. Their dissociation constants range from 12.5 to 139 nM. The classical technique for conjugation of a target to m-MPs is known to be applicable to various fields, and we conclude that this m-MP-based SELEX process provides a general strategy for screening of specific aptamers against various analytes. Graphical Abstract Rapid selection of aptamer to brain natriuretic peptide (BNP) with high affinity and specificity for rapid detection and daily monitoring of heart functions with the magnetic microparticles-based screening platform.
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doi10.1007/s00604-014-1338-y
pages331-339
date2015-01