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Gold nanoparticles based lateral flow immunoassay with largely amplified sensitivity for rapid melamine screening

The sensitivity of current lateral flow strip (LFS) based assays is a technical bottleneck. The authors describe a method for signal amplification to give a 10- to 25-fold improvement of the detection limit without compromising the assay time and operational mode. The assays includes the following s... Full description

Journal Title: Microchimica Acta 2016, Vol.183(6), pp.1989-1994
Main Author: Zhong, Youhao
Other Authors: Chen, Yinji , Yao, Li , Zhao, Diping , Zheng, Lei , Liu, Guodong , Ye, Yingwang , Chen, Wei
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0026-3672 ; E-ISSN: 1436-5073 ; DOI: 10.1007/s00604-016-1812-9
Link: http://dx.doi.org/10.1007/s00604-016-1812-9
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recordid: springer_jour10.1007/s00604-016-1812-9
title: Gold nanoparticles based lateral flow immunoassay with largely amplified sensitivity for rapid melamine screening
format: Article
creator:
  • Zhong, Youhao
  • Chen, Yinji
  • Yao, Li
  • Zhao, Diping
  • Zheng, Lei
  • Liu, Guodong
  • Ye, Yingwang
  • Chen, Wei
subjects:
  • Lateral flow strip
  • Signal amplification
  • Monoclonal antibody
  • Food safety
  • Semi-quantitative analysis
  • Milk analysis
ispartof: Microchimica Acta, 2016, Vol.183(6), pp.1989-1994
description: The sensitivity of current lateral flow strip (LFS) based assays is a technical bottleneck. The authors describe a method for signal amplification to give a 10- to 25-fold improvement of the detection limit without compromising the assay time and operational mode. The assays includes the following steps: (a) immobilization of melamine antibody on gold nanoparticles (AuNPs); (b) immobilization of BSA antibody on other AuNPs; (c) coating a nitrocellulose (NC) membrane with coating antigen and secondary antibody against the melamine antibody as the test line and control line, respectively; (d) introducing an enhancement pad for signal amplification by immuno-recognition of the blocking protein on the first conjugation pad by the antibody on the enhancement pad. This results in dual labeling of AuNPs on the test line and enhances the optical intensity of test line at the same time of detection. The signal amplification makes the optical intensity change of the test line distinguishable even at low concentrations. The assay has a detection limit of 1.4 ppb (at an S/N ratio of 3). It was applied to the determination of melamine in milk, and the results obtained with this LFS are in good agreement with those obtained with instrumental methods. This demonstrates the practical applicability of the amplified LFS to real food samples. In our perception, this approach has a wide scope because it paves the way to the systematic amplification of the sensitivity of lateral flow strips. Graphical Abstract Schematic image of signal amplified lateral flow strip (NC: nitrocellulose). One-step signal amplification strategy was adopted into traditional lateral flow strip to improve the sensing performance with the detection limit of 1.4 ppb for melamine, which is an about 25-fold improvement.
language: eng
source:
identifier: ISSN: 0026-3672 ; E-ISSN: 1436-5073 ; DOI: 10.1007/s00604-016-1812-9
fulltext: fulltext
issn:
  • 1436-5073
  • 14365073
  • 0026-3672
  • 00263672
url: Link


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titleGold nanoparticles based lateral flow immunoassay with largely amplified sensitivity for rapid melamine screening
creatorZhong, Youhao ; Chen, Yinji ; Yao, Li ; Zhao, Diping ; Zheng, Lei ; Liu, Guodong ; Ye, Yingwang ; Chen, Wei
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subjectLateral flow strip ; Signal amplification ; Monoclonal antibody ; Food safety ; Semi-quantitative analysis ; Milk analysis
descriptionThe sensitivity of current lateral flow strip (LFS) based assays is a technical bottleneck. The authors describe a method for signal amplification to give a 10- to 25-fold improvement of the detection limit without compromising the assay time and operational mode. The assays includes the following steps: (a) immobilization of melamine antibody on gold nanoparticles (AuNPs); (b) immobilization of BSA antibody on other AuNPs; (c) coating a nitrocellulose (NC) membrane with coating antigen and secondary antibody against the melamine antibody as the test line and control line, respectively; (d) introducing an enhancement pad for signal amplification by immuno-recognition of the blocking protein on the first conjugation pad by the antibody on the enhancement pad. This results in dual labeling of AuNPs on the test line and enhances the optical intensity of test line at the same time of detection. The signal amplification makes the optical intensity change of the test line distinguishable even at low concentrations. The assay has a detection limit of 1.4 ppb (at an S/N ratio of 3). It was applied to the determination of melamine in milk, and the results obtained with this LFS are in good agreement with those obtained with instrumental methods. This demonstrates the practical applicability of the amplified LFS to real food samples. In our perception, this approach has a wide scope because it paves the way to the systematic amplification of the sensitivity of lateral flow strips. Graphical Abstract Schematic image of signal amplified lateral flow strip (NC: nitrocellulose). One-step signal amplification strategy was adopted into traditional lateral flow strip to improve the sensing performance with the detection limit of 1.4 ppb for melamine, which is an about 25-fold improvement.
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0The sensitivity of current lateral flow strip (LFS) based assays is a technical bottleneck. The authors describe a method for signal amplification to give a 10- to 25-fold improvement of the detection limit without compromising the assay time and operational mode. The assays includes the following steps: (a) immobilization of melamine antibody on gold nanoparticles (AuNPs); (b) immobilization of BSA antibody on other AuNPs; (c) coating a nitrocellulose (NC) membrane with coating antigen and secondary antibody against the melamine antibody as the test line and control line, respectively; (d) introducing an enhancement pad for signal amplification by immuno-recognition of the blocking protein on the first conjugation pad by the antibody on the enhancement pad. This results in dual labeling of AuNPs on the test line and enhances the optical intensity of test line at the same time of detection. The signal amplification makes the optical intensity change of the test line distinguishable even at low concentrations. The assay has a detection limit of 1.4 ppb (at an S/N ratio of 3). It was applied to the determination of melamine in milk, and the results obtained with this LFS are in good agreement with those obtained with instrumental methods. This demonstrates the practical applicability of the amplified LFS to real food samples. In our perception, this approach has a wide scope because it paves the way to the systematic amplification of the sensitivity of lateral flow strips.
1Graphical Abstract Schematic image of signal amplified lateral flow strip (NC: nitrocellulose). One-step signal amplification strategy was adopted into traditional lateral flow strip to improve the sensing performance with the detection limit of 1.4 ppb for melamine, which is an about 25-fold improvement.
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abstractThe sensitivity of current lateral flow strip (LFS) based assays is a technical bottleneck. The authors describe a method for signal amplification to give a 10- to 25-fold improvement of the detection limit without compromising the assay time and operational mode. The assays includes the following steps: (a) immobilization of melamine antibody on gold nanoparticles (AuNPs); (b) immobilization of BSA antibody on other AuNPs; (c) coating a nitrocellulose (NC) membrane with coating antigen and secondary antibody against the melamine antibody as the test line and control line, respectively; (d) introducing an enhancement pad for signal amplification by immuno-recognition of the blocking protein on the first conjugation pad by the antibody on the enhancement pad. This results in dual labeling of AuNPs on the test line and enhances the optical intensity of test line at the same time of detection. The signal amplification makes the optical intensity change of the test line distinguishable even at low concentrations. The assay has a detection limit of 1.4 ppb (at an S/N ratio of 3). It was applied to the determination of melamine in milk, and the results obtained with this LFS are in good agreement with those obtained with instrumental methods. This demonstrates the practical applicability of the amplified LFS to real food samples. In our perception, this approach has a wide scope because it paves the way to the systematic amplification of the sensitivity of lateral flow strips. Graphical Abstract Schematic image of signal amplified lateral flow strip (NC: nitrocellulose). One-step signal amplification strategy was adopted into traditional lateral flow strip to improve the sensing performance with the detection limit of 1.4 ppb for melamine, which is an about 25-fold improvement.
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date2016-06