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Colorimetric glutathione assay based on the peroxidase-like activity of a nanocomposite consisting of platinum nanoparticles and graphene oxide

This work describes a colorimetric glutathione (GSH) assay that is taking advantage of (a) the peroxidase-like activity of a nanocomposite prepared from platinum nanoparticles and graphene oxide (PtNP/GO), (b) the decolorizing reaction of GSH with oxidized 3,3′,5,5′-tetramethylbenzidine (TMB), and (... Full description

Journal Title: Microchimica Acta 2017, Vol.184(10), pp.3945-3951
Main Author: Xu, Hui-Huang
Other Authors: Deng, Hao-Hua , Lin, Xiao-Qing , Wu, Yan-Yu , Lin, Xiu-Ling , Peng, Hua-Ping , Liu, Ai-Lin , Xia, Xing-Hua , Chen, Wei
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0026-3672 ; E-ISSN: 1436-5073 ; DOI: 10.1007/s00604-017-2429-3
Link: http://dx.doi.org/10.1007/s00604-017-2429-3
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recordid: springer_jour10.1007/s00604-017-2429-3
title: Colorimetric glutathione assay based on the peroxidase-like activity of a nanocomposite consisting of platinum nanoparticles and graphene oxide
format: Article
creator:
  • Xu, Hui-Huang
  • Deng, Hao-Hua
  • Lin, Xiao-Qing
  • Wu, Yan-Yu
  • Lin, Xiu-Ling
  • Peng, Hua-Ping
  • Liu, Ai-Lin
  • Xia, Xing-Hua
  • Chen, Wei
subjects:
  • Enzyme mimetic
  • Tetramethylbenzidine
  • Hydrogen peroxide
  • Oxidation
  • Inhibition effect
  • Haemolyzed blood
ispartof: Microchimica Acta, 2017, Vol.184(10), pp.3945-3951
description: This work describes a colorimetric glutathione (GSH) assay that is taking advantage of (a) the peroxidase-like activity of a nanocomposite prepared from platinum nanoparticles and graphene oxide (PtNP/GO), (b) the decolorizing reaction of GSH with oxidized 3,3′,5,5′-tetramethylbenzidine (TMB), and (c) the GSH-induced scavenging of hydroxy radicals produced from H 2 O 2 . The assay has a detection limit as low as 4 nM of GSH. It is flexible because it involves a single step reaction. The method was successfully applied to the determination of GSH in hemolyzed human blood. The concentrations determined by this method in GSH-spiked blood samples ranged from 95.0 to 112.5%, with relative standard deviations between 0.5 and 4.2%. Graphical abstract Schematic of a rapid and sensitive colorimetric method is developed for the detection of GSH in hemolyzed human blood. It is based on the peroxidase-mimicking property of a platinum nanoparticle/graphene oxide (PtNP/GO) composite and the inhibitory effect of GSH.
language: eng
source:
identifier: ISSN: 0026-3672 ; E-ISSN: 1436-5073 ; DOI: 10.1007/s00604-017-2429-3
fulltext: fulltext
issn:
  • 1436-5073
  • 14365073
  • 0026-3672
  • 00263672
url: Link


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titleColorimetric glutathione assay based on the peroxidase-like activity of a nanocomposite consisting of platinum nanoparticles and graphene oxide
creatorXu, Hui-Huang ; Deng, Hao-Hua ; Lin, Xiao-Qing ; Wu, Yan-Yu ; Lin, Xiu-Ling ; Peng, Hua-Ping ; Liu, Ai-Lin ; Xia, Xing-Hua ; Chen, Wei
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subjectEnzyme mimetic ; Tetramethylbenzidine ; Hydrogen peroxide ; Oxidation ; Inhibition effect ; Haemolyzed blood
descriptionThis work describes a colorimetric glutathione (GSH) assay that is taking advantage of (a) the peroxidase-like activity of a nanocomposite prepared from platinum nanoparticles and graphene oxide (PtNP/GO), (b) the decolorizing reaction of GSH with oxidized 3,3′,5,5′-tetramethylbenzidine (TMB), and (c) the GSH-induced scavenging of hydroxy radicals produced from H 2 O 2 . The assay has a detection limit as low as 4 nM of GSH. It is flexible because it involves a single step reaction. The method was successfully applied to the determination of GSH in hemolyzed human blood. The concentrations determined by this method in GSH-spiked blood samples ranged from 95.0 to 112.5%, with relative standard deviations between 0.5 and 4.2%. Graphical abstract Schematic of a rapid and sensitive colorimetric method is developed for the detection of GSH in hemolyzed human blood. It is based on the peroxidase-mimicking property of a platinum nanoparticle/graphene oxide (PtNP/GO) composite and the inhibitory effect of GSH.
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abstractThis work describes a colorimetric glutathione (GSH) assay that is taking advantage of (a) the peroxidase-like activity of a nanocomposite prepared from platinum nanoparticles and graphene oxide (PtNP/GO), (b) the decolorizing reaction of GSH with oxidized 3,3′,5,5′-tetramethylbenzidine (TMB), and (c) the GSH-induced scavenging of hydroxy radicals produced from H 2 O 2 . The assay has a detection limit as low as 4 nM of GSH. It is flexible because it involves a single step reaction. The method was successfully applied to the determination of GSH in hemolyzed human blood. The concentrations determined by this method in GSH-spiked blood samples ranged from 95.0 to 112.5%, with relative standard deviations between 0.5 and 4.2%. Graphical abstract Schematic of a rapid and sensitive colorimetric method is developed for the detection of GSH in hemolyzed human blood. It is based on the peroxidase-mimicking property of a platinum nanoparticle/graphene oxide (PtNP/GO) composite and the inhibitory effect of GSH.
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doi10.1007/s00604-017-2429-3
orcidid0000-0003-3233-8877
pages3945-3951
date2017-10