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Determination of 17β-estradiol by surface-enhanced Raman spectroscopy merged with hybridization chain reaction amplification on Au@Ag core-shell nanoparticles

The authors describe an aptamer-based assay for 17β-estradiol. It relies on the combined use of surface enhanced Raman scattering (SERS) and hybridization chain reaction (HCR). The aptamer against 17β-estradiol is applied as the recognition probes, and this results in excellent specificity. Specific... Full description

Journal Title: Microchimica Acta 2019, Vol.186(2), pp.1-9
Main Author: Yao, Li
Other Authors: Li, Yulin , Cheng, Kewen , Pan, Daodong , Xu, Jianguo , Chen, Wei
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0026-3672 ; E-ISSN: 1436-5073 ; DOI: 10.1007/s00604-018-3114-x
Link: http://dx.doi.org/10.1007/s00604-018-3114-x
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recordid: springer_jour10.1007/s00604-018-3114-x
title: Determination of 17β-estradiol by surface-enhanced Raman spectroscopy merged with hybridization chain reaction amplification on Au@Ag core-shell nanoparticles
format: Article
creator:
  • Yao, Li
  • Li, Yulin
  • Cheng, Kewen
  • Pan, Daodong
  • Xu, Jianguo
  • Chen, Wei
subjects:
  • Estrogen
  • Aptamer
  • SERS
  • Hybridization chain reaction
  • Signal amplification
  • Gold nanoparticle
  • Environment monitoring
  • Food safety
ispartof: Microchimica Acta, 2019, Vol.186(2), pp.1-9
description: The authors describe an aptamer-based assay for 17β-estradiol. It relies on the combined use of surface enhanced Raman scattering (SERS) and hybridization chain reaction (HCR). The aptamer against 17β-estradiol is applied as the recognition probes, and this results in excellent specificity. Specific recognition of target 17β-estradiol induce the freedom of DNA 2, which will open the stem-loop structure of probe 1 on the Au@Ag and form the partial dsDNA structure. With the nicking enzyme, the partial dsDNA will be hydrolyzed and the reside ssDNA on Au@Ag will form a small stem-loop structure. With the help of the other probe 2 modified Au@Ag and pre-immobilized probe 3 on the well of the microplate, an enzyme-free HCR can occur and tremendous Au@Ag can be assembled along the formed dsDNA in HCR, which can act as the excellent substrate for Raman measurement and greatly amplify the Raman signal of R6G on the Au@Ag. Afterwards, the key factor, ratio between probe 2-Au@Ag (P2) and probe1-Au@Ag (P1), affects the detection sensitivity is systematically optimized for the best sensing performance. The SERS signal of R6G, best measured at 1651 cm −1 , increases linearly in the wide range from 1 pM to 10 nM. The detection limit can be as low as 0.1 pM. Graphical abstract Schematic presentation of an aptamer-based surface enhanced Raman scattering method for accurate detection of 17β-estradiol, which is integrated with hybridization chain reaction for signal amplification and sensitivity improvement.
language: eng
source:
identifier: ISSN: 0026-3672 ; E-ISSN: 1436-5073 ; DOI: 10.1007/s00604-018-3114-x
fulltext: fulltext
issn:
  • 1436-5073
  • 14365073
  • 0026-3672
  • 00263672
url: Link


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titleDetermination of 17β-estradiol by surface-enhanced Raman spectroscopy merged with hybridization chain reaction amplification on Au@Ag core-shell nanoparticles
creatorYao, Li ; Li, Yulin ; Cheng, Kewen ; Pan, Daodong ; Xu, Jianguo ; Chen, Wei
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subjectEstrogen ; Aptamer ; SERS ; Hybridization chain reaction ; Signal amplification ; Gold nanoparticle ; Environment monitoring ; Food safety
descriptionThe authors describe an aptamer-based assay for 17β-estradiol. It relies on the combined use of surface enhanced Raman scattering (SERS) and hybridization chain reaction (HCR). The aptamer against 17β-estradiol is applied as the recognition probes, and this results in excellent specificity. Specific recognition of target 17β-estradiol induce the freedom of DNA 2, which will open the stem-loop structure of probe 1 on the Au@Ag and form the partial dsDNA structure. With the nicking enzyme, the partial dsDNA will be hydrolyzed and the reside ssDNA on Au@Ag will form a small stem-loop structure. With the help of the other probe 2 modified Au@Ag and pre-immobilized probe 3 on the well of the microplate, an enzyme-free HCR can occur and tremendous Au@Ag can be assembled along the formed dsDNA in HCR, which can act as the excellent substrate for Raman measurement and greatly amplify the Raman signal of R6G on the Au@Ag. Afterwards, the key factor, ratio between probe 2-Au@Ag (P2) and probe1-Au@Ag (P1), affects the detection sensitivity is systematically optimized for the best sensing performance. The SERS signal of R6G, best measured at 1651 cm −1 , increases linearly in the wide range from 1 pM to 10 nM. The detection limit can be as low as 0.1 pM. Graphical abstract Schematic presentation of an aptamer-based surface enhanced Raman scattering method for accurate detection of 17β-estradiol, which is integrated with hybridization chain reaction for signal amplification and sensitivity improvement.
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0The authors describe an aptamer-based assay for 17β-estradiol. It relies on the combined use of surface enhanced Raman scattering (SERS) and hybridization chain reaction (HCR). The aptamer against 17β-estradiol is applied as the recognition probes, and this results in excellent specificity. Specific recognition of target 17β-estradiol induce the freedom of DNA 2, which will open the stem-loop structure of probe 1 on the Au@Ag and form the partial dsDNA structure. With the nicking enzyme, the partial dsDNA will be hydrolyzed and the reside ssDNA on Au@Ag will form a small stem-loop structure. With the help of the other probe 2 modified Au@Ag and pre-immobilized probe 3 on the well of the microplate, an enzyme-free HCR can occur and tremendous Au@Ag can be assembled along the formed dsDNA in HCR, which can act as the excellent substrate for Raman measurement and greatly amplify the Raman signal of R6G on the Au@Ag. Afterwards, the key factor, ratio between probe 2-Au@Ag (P2) and probe1-Au@Ag (P1), affects the detection sensitivity is systematically optimized for the best sensing performance. The SERS signal of R6G, best measured at 1651 cm −1 , increases linearly in the wide range from 1 pM to 10 nM. The detection limit can be as low as 0.1 pM.
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abstractThe authors describe an aptamer-based assay for 17β-estradiol. It relies on the combined use of surface enhanced Raman scattering (SERS) and hybridization chain reaction (HCR). The aptamer against 17β-estradiol is applied as the recognition probes, and this results in excellent specificity. Specific recognition of target 17β-estradiol induce the freedom of DNA 2, which will open the stem-loop structure of probe 1 on the Au@Ag and form the partial dsDNA structure. With the nicking enzyme, the partial dsDNA will be hydrolyzed and the reside ssDNA on Au@Ag will form a small stem-loop structure. With the help of the other probe 2 modified Au@Ag and pre-immobilized probe 3 on the well of the microplate, an enzyme-free HCR can occur and tremendous Au@Ag can be assembled along the formed dsDNA in HCR, which can act as the excellent substrate for Raman measurement and greatly amplify the Raman signal of R6G on the Au@Ag. Afterwards, the key factor, ratio between probe 2-Au@Ag (P2) and probe1-Au@Ag (P1), affects the detection sensitivity is systematically optimized for the best sensing performance. The SERS signal of R6G, best measured at 1651 cm −1 , increases linearly in the wide range from 1 pM to 10 nM. The detection limit can be as low as 0.1 pM. Graphical abstract Schematic presentation of an aptamer-based surface enhanced Raman scattering method for accurate detection of 17β-estradiol, which is integrated with hybridization chain reaction for signal amplification and sensitivity improvement.
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