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Regulation of visfatin by microbial and biomechanical signals in PDL cells

Byline: Andressa Vilas Boas Nogueira (1,2), Marjan Nokhbehsaim (2,3), Sigrun Eick (4), Christoph Bourauel (3,5), Andreas Jager (3,6), Soren Jepsen (3,7), Joni Augusto Cirelli (1), James Deschner (2,3) Keywords: Biomechanics; Tension; Forces; Fusobacterium nucleatum; Visfatin; Periodontal ligament Ab... Full description

Journal Title: Clinical Oral Investigations 2014, Vol.18(1), pp.171-178
Main Author: Nogueira, Andressa
Other Authors: Nokhbehsaim, Marjan , Eick, Sigrun , Bourauel, Christoph , Jäger, Andreas , Jepsen, Søren , Cirelli, Joni , Deschner, James
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1432-6981 ; E-ISSN: 1436-3771 ; DOI: 10.1007/s00784-013-0935-1
Link: http://dx.doi.org/10.1007/s00784-013-0935-1
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recordid: springer_jour10.1007/s00784-013-0935-1
title: Regulation of visfatin by microbial and biomechanical signals in PDL cells
format: Article
creator:
  • Nogueira, Andressa
  • Nokhbehsaim, Marjan
  • Eick, Sigrun
  • Bourauel, Christoph
  • Jäger, Andreas
  • Jepsen, Søren
  • Cirelli, Joni
  • Deschner, James
subjects:
  • Biomechanics
  • Tension
  • Forces
  • Fusobacterium nucleatum
  • Visfatin
  • Periodontal ligament
ispartof: Clinical Oral Investigations, 2014, Vol.18(1), pp.171-178
description: Byline: Andressa Vilas Boas Nogueira (1,2), Marjan Nokhbehsaim (2,3), Sigrun Eick (4), Christoph Bourauel (3,5), Andreas Jager (3,6), Soren Jepsen (3,7), Joni Augusto Cirelli (1), James Deschner (2,3) Keywords: Biomechanics; Tension; Forces; Fusobacterium nucleatum; Visfatin; Periodontal ligament Abstract: Objectives This in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (PDL) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals. Materials and methods PDL cells seeded on BioFlex(r) plates were exposed to the oral pathogen Fusobacterium nucleatum ATCC 25586 and/or subjected to biomechanical strain for up to 3 days. Gene expression of visfatin and toll-like receptors (TLR) 2 and 4 was analyzed by RT-PCR, visfatin protein synthesis by ELISA and immunocytochemistry, and NFIoB nuclear translocation by immunofluorescence. Results F. nucleatum upregulated the visfatin expression in a dose- and time-dependent fashion. Preincubation with neutralizing antibodies against TLR2 and TLR4 caused a significant inhibition of the F. nucleatum-upregulated visfatin expression at 1 day. F. nucleatum stimulated the NFIoB nuclear translocation. Biomechanical loading reduced the stimulatory effects of F. nucleatum on visfatin expression at 1 and 3 days and also abrogated the F. nucleatum-induced NFIoB nuclear translocation at 60 min. Biomechanical loading inhibited significantly the expression of TLR2 and TLR4 at 3 days. The regulatory effects of F. nucleatum and/or biomechanical loading on visfatin expression were also observed at protein level. Conclusions PDL cells produce visfatin, and this production is enhanced by F. nucleatum. Biomechanical loading seems to be protective against the effects of F. nucleatum on visfatin expression. Clinical relevance Visfatin produced by periodontal tissues could play a major role in the pathogenesis of periodontitis and the interactions with obesity and other systemic diseases. Author Affiliation: (1) Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Univ Estadual Paulista -- UNESP, Araraquara, Brazil (2) Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, Germany (3) Clinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstrasse 17, 53111, Bonn, Germany (4) Department of Peri
language: eng
source:
identifier: ISSN: 1432-6981 ; E-ISSN: 1436-3771 ; DOI: 10.1007/s00784-013-0935-1
fulltext: fulltext
issn:
  • 1436-3771
  • 14363771
  • 1432-6981
  • 14326981
url: Link


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titleRegulation of visfatin by microbial and biomechanical signals in PDL cells
creatorNogueira, Andressa ; Nokhbehsaim, Marjan ; Eick, Sigrun ; Bourauel, Christoph ; Jäger, Andreas ; Jepsen, Søren ; Cirelli, Joni ; Deschner, James
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descriptionByline: Andressa Vilas Boas Nogueira (1,2), Marjan Nokhbehsaim (2,3), Sigrun Eick (4), Christoph Bourauel (3,5), Andreas Jager (3,6), Soren Jepsen (3,7), Joni Augusto Cirelli (1), James Deschner (2,3) Keywords: Biomechanics; Tension; Forces; Fusobacterium nucleatum; Visfatin; Periodontal ligament Abstract: Objectives This in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (PDL) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals. Materials and methods PDL cells seeded on BioFlex(r) plates were exposed to the oral pathogen Fusobacterium nucleatum ATCC 25586 and/or subjected to biomechanical strain for up to 3 days. Gene expression of visfatin and toll-like receptors (TLR) 2 and 4 was analyzed by RT-PCR, visfatin protein synthesis by ELISA and immunocytochemistry, and NFIoB nuclear translocation by immunofluorescence. Results F. nucleatum upregulated the visfatin expression in a dose- and time-dependent fashion. Preincubation with neutralizing antibodies against TLR2 and TLR4 caused a significant inhibition of the F. nucleatum-upregulated visfatin expression at 1 day. F. nucleatum stimulated the NFIoB nuclear translocation. Biomechanical loading reduced the stimulatory effects of F. nucleatum on visfatin expression at 1 and 3 days and also abrogated the F. nucleatum-induced NFIoB nuclear translocation at 60 min. Biomechanical loading inhibited significantly the expression of TLR2 and TLR4 at 3 days. The regulatory effects of F. nucleatum and/or biomechanical loading on visfatin expression were also observed at protein level. Conclusions PDL cells produce visfatin, and this production is enhanced by F. nucleatum. Biomechanical loading seems to be protective against the effects of F. nucleatum on visfatin expression. Clinical relevance Visfatin produced by periodontal tissues could play a major role in the pathogenesis of periodontitis and the interactions with obesity and other systemic diseases. Author Affiliation: (1) Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Univ Estadual Paulista -- UNESP, Araraquara, Brazil (2) Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, Germany (3) Clinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstrasse 17, 53111, Bonn, Germany (4) Department of Periodontology, Laboratory of Oral Microbiology, University of Bern, Bern, Switzerland (5) Oral Technology, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, Germany (6) Department of Orthodontics, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, Germany (7) Department of Periodontology, Operative and Preventive Dentistry, Center of Dento- Maxillo-Facial Medicine, University of Bonn, Bonn, Germany Article History: Registration Date: 28/01/2013 Received Date: 07/09/2012 Accepted Date: 28/01/2013 Online Date: 13/02/2013
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