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Proteomics analysis of MPP + -induced apoptosis in SH-SY5Y cells

Accumulating evidence suggests that oxidative stress plays a pivotal role in dopaminergic neurodegeneration. However, the kinds of proteins involved in the response to oxidative stress remain unclear. In the present study, SH-SY5Y cells were treated with neurotoxin 1-methyl-4-phenyl-pyridinium ion (... Full description

Journal Title: Neurological Sciences 2011, Vol.32(2), pp.221-228
Main Author: Xie, Hongrong
Other Authors: Chang, Ming , Hu, Xinyu , Wang, Danping , Tian, Mingxiu , Li, Guoyi , Jiang, Huiyi , Wang, Ying , Dong, Zhong , Zhang, Yuhua , Hu, Linsen
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1590-1874 ; E-ISSN: 1590-3478 ; DOI: 10.1007/s10072-010-0340-3
Link: http://dx.doi.org/10.1007/s10072-010-0340-3
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recordid: springer_jour10.1007/s10072-010-0340-3
title: Proteomics analysis of MPP + -induced apoptosis in SH-SY5Y cells
format: Article
creator:
  • Xie, Hongrong
  • Chang, Ming
  • Hu, Xinyu
  • Wang, Danping
  • Tian, Mingxiu
  • Li, Guoyi
  • Jiang, Huiyi
  • Wang, Ying
  • Dong, Zhong
  • Zhang, Yuhua
  • Hu, Linsen
subjects:
  • Proteomics
  • SH-SY5Y cells
  • MPP+
  • Sorcin
  • Annexin V
  • Ribosomal protein P0
ispartof: Neurological Sciences, 2011, Vol.32(2), pp.221-228
description: Accumulating evidence suggests that oxidative stress plays a pivotal role in dopaminergic neurodegeneration. However, the kinds of proteins involved in the response to oxidative stress remain unclear. In the present study, SH-SY5Y cells were treated with neurotoxin 1-methyl-4-phenyl-pyridinium ion (MPP+) to induce apoptotic neuronal injury. 2D-DIGE followed by MALDI-TOF-MS was used to determine the changing protein levels. Proteomics analysis revealed that 22 proteins were differentially altered in MPP + -treated SH-SY5Y cells, of which 7 were up-regulated proteins and 15 were down-regulated proteins, respectively. Three protein spots were unambiguously identified as sorcin, annexin V, and ribosomal protein P0. The three proteins showed a significant increase in level, suggesting a role in MPP + -induced apoptosis. The functional roles of these three proteins collectively indicate that multiple mechanisms are pertinent in the underlying pathogenesis of Parkinson’s disease (PD), such as apoptosis, calcium homeostasis, and DNA insults.
language: eng
source:
identifier: ISSN: 1590-1874 ; E-ISSN: 1590-3478 ; DOI: 10.1007/s10072-010-0340-3
fulltext: fulltext
issn:
  • 1590-3478
  • 15903478
  • 1590-1874
  • 15901874
url: Link


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titleProteomics analysis of MPP + -induced apoptosis in SH-SY5Y cells
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subjectProteomics ; SH-SY5Y cells ; MPP+ ; Sorcin ; Annexin V ; Ribosomal protein P0
descriptionAccumulating evidence suggests that oxidative stress plays a pivotal role in dopaminergic neurodegeneration. However, the kinds of proteins involved in the response to oxidative stress remain unclear. In the present study, SH-SY5Y cells were treated with neurotoxin 1-methyl-4-phenyl-pyridinium ion (MPP+) to induce apoptotic neuronal injury. 2D-DIGE followed by MALDI-TOF-MS was used to determine the changing protein levels. Proteomics analysis revealed that 22 proteins were differentially altered in MPP + -treated SH-SY5Y cells, of which 7 were up-regulated proteins and 15 were down-regulated proteins, respectively. Three protein spots were unambiguously identified as sorcin, annexin V, and ribosomal protein P0. The three proteins showed a significant increase in level, suggesting a role in MPP + -induced apoptosis. The functional roles of these three proteins collectively indicate that multiple mechanisms are pertinent in the underlying pathogenesis of Parkinson’s disease (PD), such as apoptosis, calcium homeostasis, and DNA insults.
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abstractAccumulating evidence suggests that oxidative stress plays a pivotal role in dopaminergic neurodegeneration. However, the kinds of proteins involved in the response to oxidative stress remain unclear. In the present study, SH-SY5Y cells were treated with neurotoxin 1-methyl-4-phenyl-pyridinium ion (MPP+) to induce apoptotic neuronal injury. 2D-DIGE followed by MALDI-TOF-MS was used to determine the changing protein levels. Proteomics analysis revealed that 22 proteins were differentially altered in MPP + -treated SH-SY5Y cells, of which 7 were up-regulated proteins and 15 were down-regulated proteins, respectively. Three protein spots were unambiguously identified as sorcin, annexin V, and ribosomal protein P0. The three proteins showed a significant increase in level, suggesting a role in MPP + -induced apoptosis. The functional roles of these three proteins collectively indicate that multiple mechanisms are pertinent in the underlying pathogenesis of Parkinson’s disease (PD), such as apoptosis, calcium homeostasis, and DNA insults.
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pubSpringer Milan
doi10.1007/s10072-010-0340-3
pages221-228
date2011-04