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An immunoassay using an electro-microchip, nanogold probe and silver enhancement

This paper presents a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, gold nanoparticles (ANPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. This study is based on... Full description

Journal Title: Microfluidics and Nanofluidics 2009, Vol.6(1), pp.93-98
Main Author: Su, Kuei-Ling
Other Authors: Huang, Hao-Hsuan , Chang, Tsung , Lin, Hong-Ping , Lin, Yu-Cheng , Chen, Wei-Ting
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1613-4982 ; E-ISSN: 1613-4990 ; DOI: 10.1007/s10404-008-0299-z
Link: http://dx.doi.org/10.1007/s10404-008-0299-z
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recordid: springer_jour10.1007/s10404-008-0299-z
title: An immunoassay using an electro-microchip, nanogold probe and silver enhancement
format: Article
creator:
  • Su, Kuei-Ling
  • Huang, Hao-Hsuan
  • Chang, Tsung
  • Lin, Hong-Ping
  • Lin, Yu-Cheng
  • Chen, Wei-Ting
subjects:
  • Immunoassay
  • Electro-microchip
  • Gold nanoparticles
  • Silver enhancement
ispartof: Microfluidics and Nanofluidics, 2009, Vol.6(1), pp.93-98
description: This paper presents a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, gold nanoparticles (ANPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. This study is based on the direct immunoassay (two-layer format) and the sandwich immunoassay (three-layer format). The ANPs are introduced to the electro-microchip by the specific binding of the antibodies–ANPs conjugates and then coupled with silver enhancement to produce black spots of silver metal. The silver precipitation constructs a “bridge” between two electrodes of the electro-microchip allowing the electrons to pass, and the variation of the impedance can be easily measured with a commercial LCR meter. Different gap sizes (20, 50, 100, and 200 μm) of the electrodes of electro-microchips were designed for the sensitivity study. The experimental data show that a chip with a 200 μm gap has the highest sensitivity. There is a significant difference in impedance between the experiment and the negative control after 10 min reaction time. The proposed method requires less time and fewer steps than the conventional enzyme-linked immunosorbent assay. In addition, it shows a high detection sensitivity [10 μg/mL of 1st antibody (IgG) immobilized on slides and 10 ng/mL of antigen (protein A)], and there is a clear distinction between the signal intensity and the logarithm of the sample concentration. This new immunoassay has potential applications in proteomics research and clinical diagnosis.
language: eng
source:
identifier: ISSN: 1613-4982 ; E-ISSN: 1613-4990 ; DOI: 10.1007/s10404-008-0299-z
fulltext: fulltext
issn:
  • 1613-4990
  • 16134990
  • 1613-4982
  • 16134982
url: Link


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titleAn immunoassay using an electro-microchip, nanogold probe and silver enhancement
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subjectImmunoassay ; Electro-microchip ; Gold nanoparticles ; Silver enhancement
descriptionThis paper presents a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, gold nanoparticles (ANPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. This study is based on the direct immunoassay (two-layer format) and the sandwich immunoassay (three-layer format). The ANPs are introduced to the electro-microchip by the specific binding of the antibodies–ANPs conjugates and then coupled with silver enhancement to produce black spots of silver metal. The silver precipitation constructs a “bridge” between two electrodes of the electro-microchip allowing the electrons to pass, and the variation of the impedance can be easily measured with a commercial LCR meter. Different gap sizes (20, 50, 100, and 200 μm) of the electrodes of electro-microchips were designed for the sensitivity study. The experimental data show that a chip with a 200 μm gap has the highest sensitivity. There is a significant difference in impedance between the experiment and the negative control after 10 min reaction time. The proposed method requires less time and fewer steps than the conventional enzyme-linked immunosorbent assay. In addition, it shows a high detection sensitivity [10 μg/mL of 1st antibody (IgG) immobilized on slides and 10 ng/mL of antigen (protein A)], and there is a clear distinction between the signal intensity and the logarithm of the sample concentration. This new immunoassay has potential applications in proteomics research and clinical diagnosis.
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titleAn immunoassay using an electro-microchip, nanogold probe and silver enhancement
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abstractThis paper presents a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, gold nanoparticles (ANPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. This study is based on the direct immunoassay (two-layer format) and the sandwich immunoassay (three-layer format). The ANPs are introduced to the electro-microchip by the specific binding of the antibodies–ANPs conjugates and then coupled with silver enhancement to produce black spots of silver metal. The silver precipitation constructs a “bridge” between two electrodes of the electro-microchip allowing the electrons to pass, and the variation of the impedance can be easily measured with a commercial LCR meter. Different gap sizes (20, 50, 100, and 200 μm) of the electrodes of electro-microchips were designed for the sensitivity study. The experimental data show that a chip with a 200 μm gap has the highest sensitivity. There is a significant difference in impedance between the experiment and the negative control after 10 min reaction time. The proposed method requires less time and fewer steps than the conventional enzyme-linked immunosorbent assay. In addition, it shows a high detection sensitivity [10 μg/mL of 1st antibody (IgG) immobilized on slides and 10 ng/mL of antigen (protein A)], and there is a clear distinction between the signal intensity and the logarithm of the sample concentration. This new immunoassay has potential applications in proteomics research and clinical diagnosis.
copBerlin/Heidelberg
pubSpringer-Verlag
doi10.1007/s10404-008-0299-z
pages93-98
date2009-01