schliessen

Filtern

 

Bibliotheken

Cloning and differential expression of five heat shock protein genes associated with thermal stress and development in the polyphagous predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae)

In order to explore the role of heat shock proteins (Hsps) during thermal stress and development in the predatory mite Neoseiulus cucumeris (Oudemans), we cloned and characterized five full-length Hsp genes. We investigated the expression levels of these genes by quantitative real-time PCR. The five... Full description

Journal Title: Experimental and Applied Acarology 2015, Vol.67(1), pp.65-85
Main Author: Chen, Wei
Other Authors: Li, Dunsong , Zhang, Min , Zhao, Yunlong , Wu, Wenjing , Zhang, Guren
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0168-8162 ; E-ISSN: 1572-9702 ; DOI: 10.1007/s10493-015-9933-0
Link: http://dx.doi.org/10.1007/s10493-015-9933-0
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: springer_jour10.1007/s10493-015-9933-0
title: Cloning and differential expression of five heat shock protein genes associated with thermal stress and development in the polyphagous predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae)
format: Article
creator:
  • Chen, Wei
  • Li, Dunsong
  • Zhang, Min
  • Zhao, Yunlong
  • Wu, Wenjing
  • Zhang, Guren
subjects:
  • (Oudemans)
  • Heat shock protein
  • Thermal stress
  • Developmental stage
ispartof: Experimental and Applied Acarology, 2015, Vol.67(1), pp.65-85
description: In order to explore the role of heat shock proteins (Hsps) during thermal stress and development in the predatory mite Neoseiulus cucumeris (Oudemans), we cloned and characterized five full-length Hsp genes. We investigated the expression levels of these genes by quantitative real-time PCR. The five genes characterized here were NcHsp90 , NcHsp75, NcHsp70, NcHsp60, and NcHsp40 . These Hsps showed high sequence conservation and had greatest identity with heat shock proteins of Metaseiulus occidentalis and other mite and insect species. All five NcHsp genes showed changes in their levels of expression during development. Higher levels of expression were observed in adult females than in adult males, but there were no significant changes between pre-oviposition and post-oviposition stages in the females. NcHsp90 , NcHsp75, and NcHsp70 expression levels were up-regulated after a heat shock, and the increases in NcHsp75 and NcHsp70 expression levels were maintained for at least 3 h. Up-regulation of NcHsp60 and NcHsp40 was not detected after 1 h at a high temperature (35–45 °C); however, a significant down-regulation was observed after 3 h heat exposure at 35 °C and 3 h recovery at 25 °C. Cold shock treatment (−5 to 15 °C) for 1 h did not acute elicit changes in the expression levels of any of the genes. At 5 °C, the expression levels of NcHsp90 significantly increased after 6 or 24 h exposure compared to the levels after 1 h exposure. Thus, expression of Hsp genes in N. cucumeris reflected developmental changes, sexual difference, and variable induced response to thermal stress. Increased expression of Hsps might protect N. cucumeris individuals under extreme temperature conditions. Therefore, it may be possible to enhance the thermal tolerance of commercially available N. cucumeris using temperature acclimation. Treatment at 35 °C should be suitable for such acclimation.
language: eng
source:
identifier: ISSN: 0168-8162 ; E-ISSN: 1572-9702 ; DOI: 10.1007/s10493-015-9933-0
fulltext: fulltext
issn:
  • 1572-9702
  • 15729702
  • 0168-8162
  • 01688162
url: Link


@attributes
ID64396699
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.1007/s10493-015-9933-0
sourceidspringer_jour
recordidTN_springer_jour10.1007/s10493-015-9933-0
sourcesystemOther
pqid1700106429
galeid423394904
display
typearticle
titleCloning and differential expression of five heat shock protein genes associated with thermal stress and development in the polyphagous predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae)
creatorChen, Wei ; Li, Dunsong ; Zhang, Min ; Zhao, Yunlong ; Wu, Wenjing ; Zhang, Guren
ispartofExperimental and Applied Acarology, 2015, Vol.67(1), pp.65-85
identifier
subject(Oudemans) ; Heat shock protein ; Thermal stress ; Developmental stage
descriptionIn order to explore the role of heat shock proteins (Hsps) during thermal stress and development in the predatory mite Neoseiulus cucumeris (Oudemans), we cloned and characterized five full-length Hsp genes. We investigated the expression levels of these genes by quantitative real-time PCR. The five genes characterized here were NcHsp90 , NcHsp75, NcHsp70, NcHsp60, and NcHsp40 . These Hsps showed high sequence conservation and had greatest identity with heat shock proteins of Metaseiulus occidentalis and other mite and insect species. All five NcHsp genes showed changes in their levels of expression during development. Higher levels of expression were observed in adult females than in adult males, but there were no significant changes between pre-oviposition and post-oviposition stages in the females. NcHsp90 , NcHsp75, and NcHsp70 expression levels were up-regulated after a heat shock, and the increases in NcHsp75 and NcHsp70 expression levels were maintained for at least 3 h. Up-regulation of NcHsp60 and NcHsp40 was not detected after 1 h at a high temperature (35–45 °C); however, a significant down-regulation was observed after 3 h heat exposure at 35 °C and 3 h recovery at 25 °C. Cold shock treatment (−5 to 15 °C) for 1 h did not acute elicit changes in the expression levels of any of the genes. At 5 °C, the expression levels of NcHsp90 significantly increased after 6 or 24 h exposure compared to the levels after 1 h exposure. Thus, expression of Hsp genes in N. cucumeris reflected developmental changes, sexual difference, and variable induced response to thermal stress. Increased expression of Hsps might protect N. cucumeris individuals under extreme temperature conditions. Therefore, it may be possible to enhance the thermal tolerance of commercially available N. cucumeris using temperature acclimation. Treatment at 35 °C should be suitable for such acclimation.
languageeng
source
version8
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://dx.doi.org/10.1007/s10493-015-9933-0$$EView_full_text_in_Springer_(Subscribers_only)
search
creatorcontrib
0Chen, Wei
1Li, Dunsong
2Zhang, Min
3Zhao, Yunlong
4Wu, Wenjing
5Zhang, Guren
titleCloning and differential expression of five heat shock protein genes associated with thermal stress and development in the polyphagous predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae)
descriptionIn order to explore the role of heat shock proteins (Hsps) during thermal stress and development in the predatory mite Neoseiulus cucumeris (Oudemans), we cloned and characterized five full-length Hsp genes. We investigated the expression levels of these genes by quantitative real-time PCR. The five genes characterized here were NcHsp90 , NcHsp75, NcHsp70, NcHsp60, and NcHsp40 . These Hsps showed high sequence conservation and had greatest identity with heat shock proteins of Metaseiulus occidentalis and other mite and insect species. All five NcHsp genes showed changes in their levels of expression during development. Higher levels of expression were observed in adult females than in adult males, but there were no significant changes between pre-oviposition and post-oviposition stages in the females. NcHsp90 , NcHsp75, and NcHsp70 expression levels were up-regulated after a heat shock, and the increases in NcHsp75 and NcHsp70 expression levels were maintained for at least 3 h. Up-regulation of NcHsp60 and NcHsp40 was not detected after 1 h at a high temperature (35–45 °C); however, a significant down-regulation was observed after 3 h heat exposure at 35 °C and 3 h recovery at 25 °C. Cold shock treatment (−5 to 15 °C) for 1 h did not acute elicit changes in the expression levels of any of the genes. At 5 °C, the expression levels of NcHsp90 significantly increased after 6 or 24 h exposure compared to the levels after 1 h exposure. Thus, expression of Hsp genes in N. cucumeris reflected developmental changes, sexual difference, and variable induced response to thermal stress. Increased expression of Hsps might protect N. cucumeris individuals under extreme temperature conditions. Therefore, it may be possible to enhance the thermal tolerance of commercially available N. cucumeris using temperature acclimation. Treatment at 35 °C should be suitable for such acclimation.
subject
0(Oudemans)
1Heat shock protein
2Thermal stress
3Developmental stage
general
010.1007/s10493-015-9933-0
1English
2Springer Science & Business Media B.V.
3SpringerLink
sourceidspringer_jour
recordidspringer_jour10.1007/s10493-015-9933-0
issn
01572-9702
115729702
20168-8162
301688162
rsrctypearticle
creationdate2015
addtitle
0Experimental and Applied Acarology
1Exp Appl Acarol
searchscopespringer_journals_complete
scopespringer_journals_complete
lsr30VSR-Enriched:[pages, pqid, galeid]
sort
titleCloning and differential expression of five heat shock protein genes associated with thermal stress and development in the polyphagous predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae)
authorChen, Wei ; Li, Dunsong ; Zhang, Min ; Zhao, Yunlong ; Wu, Wenjing ; Zhang, Guren
creationdate20150900
facets
frbrgroupid5794831447665626604
frbrtype5
languageeng
creationdate2015
topic
0(Oudemans)
1Heat Shock Protein
2Thermal Stress
3Developmental Stage
collectionSpringerLink
prefilterarticles
rsrctypearticles
creatorcontrib
0Chen, Wei
1Li, Dunsong
2Zhang, Min
3Zhao, Yunlong
4Wu, Wenjing
5Zhang, Guren
jtitleExperimental And Applied Acarology
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Chen
1Li
2Zhang
3Zhao
4Wu
aufirst
0Wei
1Dunsong
2Min
3Yunlong
4Wenjing
5Guren
au
0Chen, Wei
1Li, Dunsong
2Zhang, Min
3Zhao, Yunlong
4Wu, Wenjing
5Zhang, Guren
atitleCloning and differential expression of five heat shock protein genes associated with thermal stress and development in the polyphagous predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae)
jtitleExperimental and Applied Acarology
stitleExp Appl Acarol
risdate201509
volume67
issue1
spage65
epage85
issn0168-8162
eissn1572-9702
genrearticle
ristypeJOUR
abstractIn order to explore the role of heat shock proteins (Hsps) during thermal stress and development in the predatory mite Neoseiulus cucumeris (Oudemans), we cloned and characterized five full-length Hsp genes. We investigated the expression levels of these genes by quantitative real-time PCR. The five genes characterized here were NcHsp90 , NcHsp75, NcHsp70, NcHsp60, and NcHsp40 . These Hsps showed high sequence conservation and had greatest identity with heat shock proteins of Metaseiulus occidentalis and other mite and insect species. All five NcHsp genes showed changes in their levels of expression during development. Higher levels of expression were observed in adult females than in adult males, but there were no significant changes between pre-oviposition and post-oviposition stages in the females. NcHsp90 , NcHsp75, and NcHsp70 expression levels were up-regulated after a heat shock, and the increases in NcHsp75 and NcHsp70 expression levels were maintained for at least 3 h. Up-regulation of NcHsp60 and NcHsp40 was not detected after 1 h at a high temperature (35–45 °C); however, a significant down-regulation was observed after 3 h heat exposure at 35 °C and 3 h recovery at 25 °C. Cold shock treatment (−5 to 15 °C) for 1 h did not acute elicit changes in the expression levels of any of the genes. At 5 °C, the expression levels of NcHsp90 significantly increased after 6 or 24 h exposure compared to the levels after 1 h exposure. Thus, expression of Hsp genes in N. cucumeris reflected developmental changes, sexual difference, and variable induced response to thermal stress. Increased expression of Hsps might protect N. cucumeris individuals under extreme temperature conditions. Therefore, it may be possible to enhance the thermal tolerance of commercially available N. cucumeris using temperature acclimation. Treatment at 35 °C should be suitable for such acclimation.
copCham
pubSpringer International Publishing
doi10.1007/s10493-015-9933-0
pages65-85
date2015-09