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Overexpression of survivin and cyclin D1 in CHO cells confers apoptosis resistance and enhances growth in serum-free suspension culture

To optimize Chinese Hamster Ovary (CHO) cell culture to recombinant protein therapeutic production, we stably overexpressed survivin and cyclin D1 in three CHO DG44-derived cell lines. The modifications conferred increases of 56–94% in S-phase fractions and decreases of 33–43% in early-stage apoptos... Full description

Journal Title: Biotechnology Letters 2011, Vol.33(7), pp.1293-1300
Main Author: Zhao, Xinghui
Other Authors: Guo, Junwei , Yu, Yingqun , Yi, Shaoqiong , Yu, Ting , Fu, Ling , Hou, Lihua , Chen, Wei
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0141-5492 ; E-ISSN: 1573-6776 ; DOI: 10.1007/s10529-011-0577-9
Link: http://dx.doi.org/10.1007/s10529-011-0577-9
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recordid: springer_jour10.1007/s10529-011-0577-9
title: Overexpression of survivin and cyclin D1 in CHO cells confers apoptosis resistance and enhances growth in serum-free suspension culture
format: Article
creator:
  • Zhao, Xinghui
  • Guo, Junwei
  • Yu, Yingqun
  • Yi, Shaoqiong
  • Yu, Ting
  • Fu, Ling
  • Hou, Lihua
  • Chen, Wei
subjects:
  • Apoptosis
  • CHO cell line
  • Cyclin D1
  • Survivin
  • Suspension culture
ispartof: Biotechnology Letters, 2011, Vol.33(7), pp.1293-1300
description: To optimize Chinese Hamster Ovary (CHO) cell culture to recombinant protein therapeutic production, we stably overexpressed survivin and cyclin D1 in three CHO DG44-derived cell lines. The modifications conferred increases of 56–94% in S-phase fractions and decreases of 33–43% in early-stage apoptosis fractions. Clone 6.3, which expressed the highest levels of survivin and cyclin D1, reached significantly greater cell densities in suspension (2.7 × 10 6 cells/ml) following serum deprivation. Nude mice inoculated with the modified cells showed no tumorigenesis suggesting that the CHO DG44-derived cell lines are viable candidates for biopharmaceutical production.
language: eng
source:
identifier: ISSN: 0141-5492 ; E-ISSN: 1573-6776 ; DOI: 10.1007/s10529-011-0577-9
fulltext: fulltext
issn:
  • 1573-6776
  • 15736776
  • 0141-5492
  • 01415492
url: Link


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titleOverexpression of survivin and cyclin D1 in CHO cells confers apoptosis resistance and enhances growth in serum-free suspension culture
creatorZhao, Xinghui ; Guo, Junwei ; Yu, Yingqun ; Yi, Shaoqiong ; Yu, Ting ; Fu, Ling ; Hou, Lihua ; Chen, Wei
ispartofBiotechnology Letters, 2011, Vol.33(7), pp.1293-1300
identifier
subjectApoptosis ; CHO cell line ; Cyclin D1 ; Survivin ; Suspension culture
descriptionTo optimize Chinese Hamster Ovary (CHO) cell culture to recombinant protein therapeutic production, we stably overexpressed survivin and cyclin D1 in three CHO DG44-derived cell lines. The modifications conferred increases of 56–94% in S-phase fractions and decreases of 33–43% in early-stage apoptosis fractions. Clone 6.3, which expressed the highest levels of survivin and cyclin D1, reached significantly greater cell densities in suspension (2.7 × 10 6 cells/ml) following serum deprivation. Nude mice inoculated with the modified cells showed no tumorigenesis suggesting that the CHO DG44-derived cell lines are viable candidates for biopharmaceutical production.
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titleOverexpression of survivin and cyclin D1 in CHO cells confers apoptosis resistance and enhances growth in serum-free suspension culture
descriptionTo optimize Chinese Hamster Ovary (CHO) cell culture to recombinant protein therapeutic production, we stably overexpressed survivin and cyclin D1 in three CHO DG44-derived cell lines. The modifications conferred increases of 56–94% in S-phase fractions and decreases of 33–43% in early-stage apoptosis fractions. Clone 6.3, which expressed the highest levels of survivin and cyclin D1, reached significantly greater cell densities in suspension (2.7 × 10 6 cells/ml) following serum deprivation. Nude mice inoculated with the modified cells showed no tumorigenesis suggesting that the CHO DG44-derived cell lines are viable candidates for biopharmaceutical production.
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titleOverexpression of survivin and cyclin D1 in CHO cells confers apoptosis resistance and enhances growth in serum-free suspension culture
authorZhao, Xinghui ; Guo, Junwei ; Yu, Yingqun ; Yi, Shaoqiong ; Yu, Ting ; Fu, Ling ; Hou, Lihua ; Chen, Wei
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abstractTo optimize Chinese Hamster Ovary (CHO) cell culture to recombinant protein therapeutic production, we stably overexpressed survivin and cyclin D1 in three CHO DG44-derived cell lines. The modifications conferred increases of 56–94% in S-phase fractions and decreases of 33–43% in early-stage apoptosis fractions. Clone 6.3, which expressed the highest levels of survivin and cyclin D1, reached significantly greater cell densities in suspension (2.7 × 10 6 cells/ml) following serum deprivation. Nude mice inoculated with the modified cells showed no tumorigenesis suggesting that the CHO DG44-derived cell lines are viable candidates for biopharmaceutical production.
copDordrecht
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doi10.1007/s10529-011-0577-9
pages1293-1300
date2011-07