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Silencing of GATA6 Suppresses SW1990 Pancreatic Cancer Cell Growth In Vitro and Up-Regulates Reactive Oxygen Species

Byline: Wen-Bo Chen (1), Feng-Ting Huang (1,2), Yan-Yan Zhuang (1), Jian Tang (3), Xiao-Hong Zhuang (4), Wen-Jie Cheng (1), Zhi-Qiang Gu (1), Shi-Neng Zhang (1) Keywords: Pancreatic cancer; GATA6; Reactive oxygen species; RNA interference; Growth Abstract: Background/Aims Pancreatic cancer has the w... Full description

Journal Title: Digestive Diseases and Sciences 2013, Vol.58(9), pp.2518-2527
Main Author: Chen, Wen-Bo
Other Authors: Huang, Feng-Ting , Zhuang, Yan-Yan , Tang, Jian , Zhuang, Xiao-Hong , Cheng, Wen-Jie , Gu, Zhi-Qiang , Zhang, Shi-Neng
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0163-2116 ; E-ISSN: 1573-2568 ; DOI: 10.1007/s10620-013-2752-4
Link: http://dx.doi.org/10.1007/s10620-013-2752-4
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recordid: springer_jour10.1007/s10620-013-2752-4
title: Silencing of GATA6 Suppresses SW1990 Pancreatic Cancer Cell Growth In Vitro and Up-Regulates Reactive Oxygen Species
format: Article
creator:
  • Chen, Wen-Bo
  • Huang, Feng-Ting
  • Zhuang, Yan-Yan
  • Tang, Jian
  • Zhuang, Xiao-Hong
  • Cheng, Wen-Jie
  • Gu, Zhi-Qiang
  • Zhang, Shi-Neng
subjects:
  • Pancreatic cancer
  • GATA6
  • Reactive oxygen species
  • RNA interference
  • Growth
ispartof: Digestive Diseases and Sciences, 2013, Vol.58(9), pp.2518-2527
description: Byline: Wen-Bo Chen (1), Feng-Ting Huang (1,2), Yan-Yan Zhuang (1), Jian Tang (3), Xiao-Hong Zhuang (4), Wen-Jie Cheng (1), Zhi-Qiang Gu (1), Shi-Neng Zhang (1) Keywords: Pancreatic cancer; GATA6; Reactive oxygen species; RNA interference; Growth Abstract: Background/Aims Pancreatic cancer has the worst prognosis of any gastrointestinal cancer with a mortality rate approaching its incidence. Previous studies have indicated that GATA6 plays a key role in organ development and function, and that abnormal expression of GATA6 may induce tumorigenesis. Meanwhile, it has been reported that generation of reactive oxygen species contributes to carcinogenesis. In this study, we set out to study the role of GATA6 expression on proliferation and apoptosis of pancreatic cancer cells and the role of reactive oxygen species. Methods Four target miRNA sequences against GATA6 mRNA were synthesized and used to transfect SW1990 cells. Then, GATA6 expression in SW1990 cells was examined by western blot and quantative real-time polymerase chain reaction. Cell proliferation was examined by WST-8 and colony formation assay. Cell cycle progression and apoptosis were measured by flow cytometry. We also measured the generation of reactive oxygen species by immunofluorescence and flow cytometry. Results RNA interference against GATA6 successfully inhibited mRNA and protein expression of GATA6 in the SW1990 pancreatic cancer cell line. Silencing of GATA6 by RNA interference inhibited cell proliferation and increased apoptosis of SW1990, and enhanced the expression of reactive oxygen species. Conclusions These results suggest that the RNA interference approach against GATA6 may be an effective therapeutic approach for treatment of pancreatic cancer. Author Affiliation: (1) Department of Gastroenterology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, No. 107 Yanjiang West Road, Guangzhou, 510120, Guangdong, People's Republic of China (2) Institute of Gastroenterology and the Sixth Affiliated Hospital, Institute of Human Virology, Key Laboratory of Tropical Disease Control (Ministry of Education), Sun Yat-sen University, No. 26 Yuancun Erheng Road, Guangzhou, 510655, Guangdong, People's Republic of China (3) Department of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, No. 26 Yuancun Erheng Road, Guangzhou, 510655, Guangdong, People's Republic of China (4) Department of Oncology and Hematology, Hainan Provincial Nongken Hospital, No. 48 Building Baishu
language: eng
source:
identifier: ISSN: 0163-2116 ; E-ISSN: 1573-2568 ; DOI: 10.1007/s10620-013-2752-4
fulltext: fulltext
issn:
  • 1573-2568
  • 15732568
  • 0163-2116
  • 01632116
url: Link


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titleSilencing of GATA6 Suppresses SW1990 Pancreatic Cancer Cell Growth In Vitro and Up-Regulates Reactive Oxygen Species
creatorChen, Wen-Bo ; Huang, Feng-Ting ; Zhuang, Yan-Yan ; Tang, Jian ; Zhuang, Xiao-Hong ; Cheng, Wen-Jie ; Gu, Zhi-Qiang ; Zhang, Shi-Neng
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descriptionByline: Wen-Bo Chen (1), Feng-Ting Huang (1,2), Yan-Yan Zhuang (1), Jian Tang (3), Xiao-Hong Zhuang (4), Wen-Jie Cheng (1), Zhi-Qiang Gu (1), Shi-Neng Zhang (1) Keywords: Pancreatic cancer; GATA6; Reactive oxygen species; RNA interference; Growth Abstract: Background/Aims Pancreatic cancer has the worst prognosis of any gastrointestinal cancer with a mortality rate approaching its incidence. Previous studies have indicated that GATA6 plays a key role in organ development and function, and that abnormal expression of GATA6 may induce tumorigenesis. Meanwhile, it has been reported that generation of reactive oxygen species contributes to carcinogenesis. In this study, we set out to study the role of GATA6 expression on proliferation and apoptosis of pancreatic cancer cells and the role of reactive oxygen species. Methods Four target miRNA sequences against GATA6 mRNA were synthesized and used to transfect SW1990 cells. Then, GATA6 expression in SW1990 cells was examined by western blot and quantative real-time polymerase chain reaction. Cell proliferation was examined by WST-8 and colony formation assay. Cell cycle progression and apoptosis were measured by flow cytometry. We also measured the generation of reactive oxygen species by immunofluorescence and flow cytometry. Results RNA interference against GATA6 successfully inhibited mRNA and protein expression of GATA6 in the SW1990 pancreatic cancer cell line. Silencing of GATA6 by RNA interference inhibited cell proliferation and increased apoptosis of SW1990, and enhanced the expression of reactive oxygen species. Conclusions These results suggest that the RNA interference approach against GATA6 may be an effective therapeutic approach for treatment of pancreatic cancer. Author Affiliation: (1) Department of Gastroenterology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, No. 107 Yanjiang West Road, Guangzhou, 510120, Guangdong, People's Republic of China (2) Institute of Gastroenterology and the Sixth Affiliated Hospital, Institute of Human Virology, Key Laboratory of Tropical Disease Control (Ministry of Education), Sun Yat-sen University, No. 26 Yuancun Erheng Road, Guangzhou, 510655, Guangdong, People's Republic of China (3) Department of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, No. 26 Yuancun Erheng Road, Guangzhou, 510655, Guangdong, People's Republic of China (4) Department of Oncology and Hematology, Hainan Provincial Nongken Hospital, No. 48 Building Baishuitang Road, Haikou, 570311, Hainan, People's Republic of China Article History: Registration Date: 06/06/2013 Received Date: 23/09/2012 Accepted Date: 06/06/2013 Online Date: 06/07/2013 Article note: Wen-Bo Chen and Feng-Ting Huang contributed equally to this work.
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