schliessen

Filtern

 

Bibliotheken

Synthesis and Assessment of DNA/Silver Nanoclusters Probes for Optimal and Selective Detection of Tristeza Virus Mild Strains

Citrus Tristeza virus (CTV) is one of the most destructive pathogens worldwide that exist as a mixture of malicious (Sever) and tolerable (Mild) strains. Mild strains of CTV can be used to immunize healthy plants from more Severe strains damage. Recently, innovative methods based on the fluorescent... Full description

Journal Title: Journal of Fluorescence 2016, Vol.26(5), pp.1795-1803
Main Author: Shokri, Ehsan
Other Authors: Hosseini, Morteza , Faridbod, Farnoush , Rahaie, Mahdi
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1053-0509 ; E-ISSN: 1573-4994 ; DOI: 10.1007/s10895-016-1871-7
Link: http://dx.doi.org/10.1007/s10895-016-1871-7
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: springer_jour10.1007/s10895-016-1871-7
title: Synthesis and Assessment of DNA/Silver Nanoclusters Probes for Optimal and Selective Detection of Tristeza Virus Mild Strains
format: Article
creator:
  • Shokri, Ehsan
  • Hosseini, Morteza
  • Faridbod, Farnoush
  • Rahaie, Mahdi
subjects:
  • Tristeza virus
  • Mild strains
  • DNA/AgNCs
  • Emission drop
  • Optimization
ispartof: Journal of Fluorescence, 2016, Vol.26(5), pp.1795-1803
description: Citrus Tristeza virus (CTV) is one of the most destructive pathogens worldwide that exist as a mixture of malicious (Sever) and tolerable (Mild) strains. Mild strains of CTV can be used to immunize healthy plants from more Severe strains damage. Recently, innovative methods based on the fluorescent properties of DNA/silver nanoclusters have been developed for molecular detection purposes. In this study, a simple procedure was followed to create more active DNA/AgNCs probe for accurate and selective detection of Tristeza Mild-RNA. To this end, four distinct DNA emitter scaffolds (C12, Red, Green, Yellow) were tethered to the Mild capture sequence and investigated in various buffers in order to find highly emissive combinations. Then, to achieve specific and reliable results, several chemical additives, including organic solvents, PEG and organo-soluble salts were used to enhance control fluorescence signals and optimize the hybridization solution. The data showed that, under adjusted conditions, the target sensitivity is enhanced by a factor of five and the high discrimination between Mild and Severe RNAs were obtained. The emission ratio of the DNA/AgNCs was dropped in the presence of target RNAs and I 0 /I intensity linearly ranged from 1.5 × 10 −8  M to 1.8 × 10 −6  M with the detection limit of 4.3 × 10 −9  M.
language: eng
source:
identifier: ISSN: 1053-0509 ; E-ISSN: 1573-4994 ; DOI: 10.1007/s10895-016-1871-7
fulltext: fulltext
issn:
  • 1573-4994
  • 15734994
  • 1053-0509
  • 10530509
url: Link


@attributes
ID1447837995
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.1007/s10895-016-1871-7
sourceidspringer_jour
recordidTN_springer_jour10.1007/s10895-016-1871-7
sourcesystemPC
pqid1880883758
galeid460846967
display
typearticle
titleSynthesis and Assessment of DNA/Silver Nanoclusters Probes for Optimal and Selective Detection of Tristeza Virus Mild Strains
creatorShokri, Ehsan ; Hosseini, Morteza ; Faridbod, Farnoush ; Rahaie, Mahdi
ispartofJournal of Fluorescence, 2016, Vol.26(5), pp.1795-1803
identifier
subjectTristeza virus ; Mild strains ; DNA/AgNCs ; Emission drop ; Optimization
descriptionCitrus Tristeza virus (CTV) is one of the most destructive pathogens worldwide that exist as a mixture of malicious (Sever) and tolerable (Mild) strains. Mild strains of CTV can be used to immunize healthy plants from more Severe strains damage. Recently, innovative methods based on the fluorescent properties of DNA/silver nanoclusters have been developed for molecular detection purposes. In this study, a simple procedure was followed to create more active DNA/AgNCs probe for accurate and selective detection of Tristeza Mild-RNA. To this end, four distinct DNA emitter scaffolds (C12, Red, Green, Yellow) were tethered to the Mild capture sequence and investigated in various buffers in order to find highly emissive combinations. Then, to achieve specific and reliable results, several chemical additives, including organic solvents, PEG and organo-soluble salts were used to enhance control fluorescence signals and optimize the hybridization solution. The data showed that, under adjusted conditions, the target sensitivity is enhanced by a factor of five and the high discrimination between Mild and Severe RNAs were obtained. The emission ratio of the DNA/AgNCs was dropped in the presence of target RNAs and I 0 /I intensity linearly ranged from 1.5 × 10 −8  M to 1.8 × 10 −6  M with the detection limit of 4.3 × 10 −9  M.
languageeng
source
version6
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://dx.doi.org/10.1007/s10895-016-1871-7$$EView_full_text_in_Springer_(Subscribers_only)
search
creatorcontrib
0Shokri, Ehsan
1Hosseini, Morteza
2Faridbod, Farnoush
3Rahaie, Mahdi
titleSynthesis and Assessment of DNA/Silver Nanoclusters Probes for Optimal and Selective Detection of Tristeza Virus Mild Strains
descriptionCitrus Tristeza virus (CTV) is one of the most destructive pathogens worldwide that exist as a mixture of malicious (Sever) and tolerable (Mild) strains. Mild strains of CTV can be used to immunize healthy plants from more Severe strains damage. Recently, innovative methods based on the fluorescent properties of DNA/silver nanoclusters have been developed for molecular detection purposes. In this study, a simple procedure was followed to create more active DNA/AgNCs probe for accurate and selective detection of Tristeza Mild-RNA. To this end, four distinct DNA emitter scaffolds (C12, Red, Green, Yellow) were tethered to the Mild capture sequence and investigated in various buffers in order to find highly emissive combinations. Then, to achieve specific and reliable results, several chemical additives, including organic solvents, PEG and organo-soluble salts were used to enhance control fluorescence signals and optimize the hybridization solution. The data showed that, under adjusted conditions, the target sensitivity is enhanced by a factor of five and the high discrimination between Mild and Severe RNAs were obtained. The emission ratio of the DNA/AgNCs was dropped in the presence of target RNAs and I 0 /I intensity linearly ranged from 1.5 × 10 −8  M to 1.8 × 10 −6  M with the detection limit of 4.3 × 10 −9  M.
subject
0Tristeza virus
1Mild strains
2DNA/AgNCs
3Emission drop
4Optimization
general
010.1007/s10895-016-1871-7
1English
2Springer Science & Business Media B.V.
3SpringerLink
sourceidspringer_jour
recordidspringer_jour10.1007/s10895-016-1871-7
issn
01573-4994
115734994
21053-0509
310530509
rsrctypearticle
creationdate2016
addtitle
0Journal of Fluorescence
1J Fluoresc
searchscopespringer_journals_complete
scopespringer_journals_complete
lsr30VSR-Enriched:[pages, pqid, galeid]
sort
titleSynthesis and Assessment of DNA/Silver Nanoclusters Probes for Optimal and Selective Detection of Tristeza Virus Mild Strains
authorShokri, Ehsan ; Hosseini, Morteza ; Faridbod, Farnoush ; Rahaie, Mahdi
creationdate20160900
facets
frbrgroupid-1064062838011628138
frbrtype5
languageeng
creationdate2016
topic
0Tristeza Virus
1Mild Strains
2Dna/Agncs
3Emission Drop
4Optimization
collectionSpringerLink
prefilterarticles
rsrctypearticles
creatorcontrib
0Shokri, Ehsan
1Hosseini, Morteza
2Faridbod, Farnoush
3Rahaie, Mahdi
jtitleJournal Of Fluorescence
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Shokri
1Hosseini
2Faridbod
3Rahaie
aufirst
0Ehsan
1Morteza
2Farnoush
3Mahdi
au
0Shokri, Ehsan
1Hosseini, Morteza
2Faridbod, Farnoush
3Rahaie, Mahdi
atitleSynthesis and Assessment of DNA/Silver Nanoclusters Probes for Optimal and Selective Detection of Tristeza Virus Mild Strains
jtitleJournal of Fluorescence
stitleJ Fluoresc
risdate201609
volume26
issue5
spage1795
epage1803
issn1053-0509
eissn1573-4994
genrearticle
ristypeJOUR
abstractCitrus Tristeza virus (CTV) is one of the most destructive pathogens worldwide that exist as a mixture of malicious (Sever) and tolerable (Mild) strains. Mild strains of CTV can be used to immunize healthy plants from more Severe strains damage. Recently, innovative methods based on the fluorescent properties of DNA/silver nanoclusters have been developed for molecular detection purposes. In this study, a simple procedure was followed to create more active DNA/AgNCs probe for accurate and selective detection of Tristeza Mild-RNA. To this end, four distinct DNA emitter scaffolds (C12, Red, Green, Yellow) were tethered to the Mild capture sequence and investigated in various buffers in order to find highly emissive combinations. Then, to achieve specific and reliable results, several chemical additives, including organic solvents, PEG and organo-soluble salts were used to enhance control fluorescence signals and optimize the hybridization solution. The data showed that, under adjusted conditions, the target sensitivity is enhanced by a factor of five and the high discrimination between Mild and Severe RNAs were obtained. The emission ratio of the DNA/AgNCs was dropped in the presence of target RNAs and I 0 /I intensity linearly ranged from 1.5 × 10 −8  M to 1.8 × 10 −6  M with the detection limit of 4.3 × 10 −9  M.
copNew York
pubSpringer US
doi10.1007/s10895-016-1871-7
pages1795-1803
date2016-09