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Detection of p53 Gene Mutation (Single-Base Mismatch) Using a Fluorescent Silver Nanoclusters

P53 mutation was detected through the application of a biosensing approach based on the decrease in the fluorescence of oligonucleotide-templated silver nanoclusters (DNA-AgNCs). To this end specific DNA scaffolds of two various nucleotide fragments were used. One of the scaffolds was enriched with... Full description

Journal Title: Journal of Fluorescence 2017, Vol.27(4), pp.1443-1448
Main Author: Hosseini, Morteza
Other Authors: Mohammadi, Shiva , Borghei, Yasaman-Sadat , Ganjali, Mohammad
Format: Electronic Article Electronic Article
Language: English
Subjects:
p53
ID: ISSN: 1053-0509 ; E-ISSN: 1573-4994 ; DOI: 10.1007/s10895-017-2083-5
Link: http://dx.doi.org/10.1007/s10895-017-2083-5
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recordid: springer_jour10.1007/s10895-017-2083-5
title: Detection of p53 Gene Mutation (Single-Base Mismatch) Using a Fluorescent Silver Nanoclusters
format: Article
creator:
  • Hosseini, Morteza
  • Mohammadi, Shiva
  • Borghei, Yasaman-Sadat
  • Ganjali, Mohammad
subjects:
  • Mutation
  • p53
  • Silver nanoclusters
  • Nanobiosensor
  • Fluorescence
ispartof: Journal of Fluorescence, 2017, Vol.27(4), pp.1443-1448
description: P53 mutation was detected through the application of a biosensing approach based on the decrease in the fluorescence of oligonucleotide-templated silver nanoclusters (DNA-AgNCs). To this end specific DNA scaffolds of two various nucleotide fragments were used. One of the scaffolds was enriched with two cytosine sequence fragment (C12). This led to DNA-AgNCs with a fluorescence intensity through chemical reduction, while the other scaffold acted as the probe fragment (5- GTAGATGGCCATGGCGCGGACGCGGGTG-3). This latter scaffold selectively bound to the specific p53 site. Thus, resulting AgNCs demonstrated decreased fluorescence upon binding to single-base mismatching targets, and this behavior was found to be linearly proportional to the concentration of mutated p53 from 5 to 350 nM and the approach was found to be able to detect concentrations as low as 1.3 nM.
language: eng
source:
identifier: ISSN: 1053-0509 ; E-ISSN: 1573-4994 ; DOI: 10.1007/s10895-017-2083-5
fulltext: fulltext
issn:
  • 1573-4994
  • 15734994
  • 1053-0509
  • 10530509
url: Link


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titleDetection of p53 Gene Mutation (Single-Base Mismatch) Using a Fluorescent Silver Nanoclusters
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subjectMutation ; p53 ; Silver nanoclusters ; Nanobiosensor ; Fluorescence
descriptionP53 mutation was detected through the application of a biosensing approach based on the decrease in the fluorescence of oligonucleotide-templated silver nanoclusters (DNA-AgNCs). To this end specific DNA scaffolds of two various nucleotide fragments were used. One of the scaffolds was enriched with two cytosine sequence fragment (C12). This led to DNA-AgNCs with a fluorescence intensity through chemical reduction, while the other scaffold acted as the probe fragment (5- GTAGATGGCCATGGCGCGGACGCGGGTG-3). This latter scaffold selectively bound to the specific p53 site. Thus, resulting AgNCs demonstrated decreased fluorescence upon binding to single-base mismatching targets, and this behavior was found to be linearly proportional to the concentration of mutated p53 from 5 to 350 nM and the approach was found to be able to detect concentrations as low as 1.3 nM.
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titleDetection of p53 Gene Mutation (Single-Base Mismatch) Using a Fluorescent Silver Nanoclusters
descriptionP53 mutation was detected through the application of a biosensing approach based on the decrease in the fluorescence of oligonucleotide-templated silver nanoclusters (DNA-AgNCs). To this end specific DNA scaffolds of two various nucleotide fragments were used. One of the scaffolds was enriched with two cytosine sequence fragment (C12). This led to DNA-AgNCs with a fluorescence intensity through chemical reduction, while the other scaffold acted as the probe fragment (5- GTAGATGGCCATGGCGCGGACGCGGGTG-3). This latter scaffold selectively bound to the specific p53 site. Thus, resulting AgNCs demonstrated decreased fluorescence upon binding to single-base mismatching targets, and this behavior was found to be linearly proportional to the concentration of mutated p53 from 5 to 350 nM and the approach was found to be able to detect concentrations as low as 1.3 nM.
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abstractP53 mutation was detected through the application of a biosensing approach based on the decrease in the fluorescence of oligonucleotide-templated silver nanoclusters (DNA-AgNCs). To this end specific DNA scaffolds of two various nucleotide fragments were used. One of the scaffolds was enriched with two cytosine sequence fragment (C12). This led to DNA-AgNCs with a fluorescence intensity through chemical reduction, while the other scaffold acted as the probe fragment (5- GTAGATGGCCATGGCGCGGACGCGGGTG-3). This latter scaffold selectively bound to the specific p53 site. Thus, resulting AgNCs demonstrated decreased fluorescence upon binding to single-base mismatching targets, and this behavior was found to be linearly proportional to the concentration of mutated p53 from 5 to 350 nM and the approach was found to be able to detect concentrations as low as 1.3 nM.
copNew York
pubSpringer US
doi10.1007/s10895-017-2083-5
pages1443-1448
date2017-07