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Chronic mild hyperhomocysteinemia alters ectonucleotidase activities and gene expression of ecto-5′-nucleotidase/CD73 in rat lymphocytes

Since mild hyperhomocysteinemia is a risk factor for cardiovascular and cerebral diseases and extracellular nucleotides/nucleosides, which are controlled by the enzymatic action of ectonucleotidases, can induce an immune response, in the present study, we investigated the effect of chronic mild hype... Full description

Journal Title: Molecular and Cellular Biochemistry 2012, Vol.362(1), pp.187-194
Main Author: Scherer, Emilene
Other Authors: Savio, Luiz , Vuaden, Fernanda , Ferreira, Andréa , Bogo, Maurício , Bonan, Carla , Wyse, Angela
Format: Electronic Article Electronic Article
Language: English
Subjects:
ATP
ID: ISSN: 0300-8177 ; E-ISSN: 1573-4919 ; DOI: 10.1007/s11010-011-1141-6
Link: http://dx.doi.org/10.1007/s11010-011-1141-6
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recordid: springer_jour10.1007/s11010-011-1141-6
title: Chronic mild hyperhomocysteinemia alters ectonucleotidase activities and gene expression of ecto-5′-nucleotidase/CD73 in rat lymphocytes
format: Article
creator:
  • Scherer, Emilene
  • Savio, Luiz
  • Vuaden, Fernanda
  • Ferreira, Andréa
  • Bogo, Maurício
  • Bonan, Carla
  • Wyse, Angela
subjects:
  • Mild hyperhomocysteinemia
  • Lymphocytes
  • Ectonucleotidases
  • ATP
  • Adenosine
ispartof: Molecular and Cellular Biochemistry, 2012, Vol.362(1), pp.187-194
description: Since mild hyperhomocysteinemia is a risk factor for cardiovascular and cerebral diseases and extracellular nucleotides/nucleosides, which are controlled by the enzymatic action of ectonucleotidases, can induce an immune response, in the present study, we investigated the effect of chronic mild hyperhomocysteinemia on ectonucleotidase activities and expression in lymphocytes from mesenteric lymph nodes and serum of adult rats. For the chronic chemically induced mild hyperhomocysteinemia, Hcy (0.03 μmol/g of body weight) or saline (control) were administered subcutaneously from the 30th to the 60th day of life. Results showed that homocysteine significantly decreased ATP, ADP, and AMP hydrolysis in lymphocytes of adult rats. E-NTPDases transcriptions were not affected, while the ecto-5′-nucleotidase transcription was significantly decreased in mesenteric lymph nodes of hyperhomocysteinemic rats. ATP, ADP, and AMP hydrolysis were not affected by homocysteine in rat serum. Our findings suggest that Hcy in levels similar to considered risk factor to development of vascular diseases modulates the ectonucleotidases, which could lead to a pro-inflammatory status.
language: eng
source:
identifier: ISSN: 0300-8177 ; E-ISSN: 1573-4919 ; DOI: 10.1007/s11010-011-1141-6
fulltext: fulltext
issn:
  • 1573-4919
  • 15734919
  • 0300-8177
  • 03008177
url: Link


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titleChronic mild hyperhomocysteinemia alters ectonucleotidase activities and gene expression of ecto-5′-nucleotidase/CD73 in rat lymphocytes
creatorScherer, Emilene ; Savio, Luiz ; Vuaden, Fernanda ; Ferreira, Andréa ; Bogo, Maurício ; Bonan, Carla ; Wyse, Angela
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subjectMild hyperhomocysteinemia ; Lymphocytes ; Ectonucleotidases ; ATP ; Adenosine
descriptionSince mild hyperhomocysteinemia is a risk factor for cardiovascular and cerebral diseases and extracellular nucleotides/nucleosides, which are controlled by the enzymatic action of ectonucleotidases, can induce an immune response, in the present study, we investigated the effect of chronic mild hyperhomocysteinemia on ectonucleotidase activities and expression in lymphocytes from mesenteric lymph nodes and serum of adult rats. For the chronic chemically induced mild hyperhomocysteinemia, Hcy (0.03 μmol/g of body weight) or saline (control) were administered subcutaneously from the 30th to the 60th day of life. Results showed that homocysteine significantly decreased ATP, ADP, and AMP hydrolysis in lymphocytes of adult rats. E-NTPDases transcriptions were not affected, while the ecto-5′-nucleotidase transcription was significantly decreased in mesenteric lymph nodes of hyperhomocysteinemic rats. ATP, ADP, and AMP hydrolysis were not affected by homocysteine in rat serum. Our findings suggest that Hcy in levels similar to considered risk factor to development of vascular diseases modulates the ectonucleotidases, which could lead to a pro-inflammatory status.
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titleChronic mild hyperhomocysteinemia alters ectonucleotidase activities and gene expression of ecto-5′-nucleotidase/CD73 in rat lymphocytes
descriptionSince mild hyperhomocysteinemia is a risk factor for cardiovascular and cerebral diseases and extracellular nucleotides/nucleosides, which are controlled by the enzymatic action of ectonucleotidases, can induce an immune response, in the present study, we investigated the effect of chronic mild hyperhomocysteinemia on ectonucleotidase activities and expression in lymphocytes from mesenteric lymph nodes and serum of adult rats. For the chronic chemically induced mild hyperhomocysteinemia, Hcy (0.03 μmol/g of body weight) or saline (control) were administered subcutaneously from the 30th to the 60th day of life. Results showed that homocysteine significantly decreased ATP, ADP, and AMP hydrolysis in lymphocytes of adult rats. E-NTPDases transcriptions were not affected, while the ecto-5′-nucleotidase transcription was significantly decreased in mesenteric lymph nodes of hyperhomocysteinemic rats. ATP, ADP, and AMP hydrolysis were not affected by homocysteine in rat serum. Our findings suggest that Hcy in levels similar to considered risk factor to development of vascular diseases modulates the ectonucleotidases, which could lead to a pro-inflammatory status.
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abstractSince mild hyperhomocysteinemia is a risk factor for cardiovascular and cerebral diseases and extracellular nucleotides/nucleosides, which are controlled by the enzymatic action of ectonucleotidases, can induce an immune response, in the present study, we investigated the effect of chronic mild hyperhomocysteinemia on ectonucleotidase activities and expression in lymphocytes from mesenteric lymph nodes and serum of adult rats. For the chronic chemically induced mild hyperhomocysteinemia, Hcy (0.03 μmol/g of body weight) or saline (control) were administered subcutaneously from the 30th to the 60th day of life. Results showed that homocysteine significantly decreased ATP, ADP, and AMP hydrolysis in lymphocytes of adult rats. E-NTPDases transcriptions were not affected, while the ecto-5′-nucleotidase transcription was significantly decreased in mesenteric lymph nodes of hyperhomocysteinemic rats. ATP, ADP, and AMP hydrolysis were not affected by homocysteine in rat serum. Our findings suggest that Hcy in levels similar to considered risk factor to development of vascular diseases modulates the ectonucleotidases, which could lead to a pro-inflammatory status.
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pubSpringer US
doi10.1007/s11010-011-1141-6
pages187-194
date2012-03