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High-throughput single nucleotide polymorphism genotyping for breeding applications in rice using the BeadXpress platform

Multiplexed single nucleotide polymorphism (SNP) markers have the potential to increase the speed and cost-effectiveness of genotyping, provided that an optimal SNP density is used for each application. To test the efficiency of multiplexed SNP genotyping for diversity, mapping and breeding applicat... Full description

Journal Title: Molecular Breeding 2012, Vol.29(4), pp.875-886
Main Author: Thomson, Michael
Other Authors: Zhao, Keyan , Wright, Mark , McNally, Kenneth , Rey, Jessica , Tung, Chih-Wei , Reynolds, Andy , Scheffler, Brian , Eizenga, Georgia , McClung, Anna , Kim, Hyunjung , Ismail, Abdelbagi , Ocampo, Marjorie , Mojica, Chromewell , Reveche, Ma. , Dilla-Ermita, Christine , Mauleon, Ramil , Leung, Hei , Bustamante, Carlos , McCouch, Susan
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1380-3743 ; E-ISSN: 1572-9788 ; DOI: 10.1007/s11032-011-9663-x
Link: http://dx.doi.org/10.1007/s11032-011-9663-x
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recordid: springer_jour10.1007/s11032-011-9663-x
title: High-throughput single nucleotide polymorphism genotyping for breeding applications in rice using the BeadXpress platform
format: Article
creator:
  • Thomson, Michael
  • Zhao, Keyan
  • Wright, Mark
  • McNally, Kenneth
  • Rey, Jessica
  • Tung, Chih-Wei
  • Reynolds, Andy
  • Scheffler, Brian
  • Eizenga, Georgia
  • McClung, Anna
  • Kim, Hyunjung
  • Ismail, Abdelbagi
  • Ocampo, Marjorie
  • Mojica, Chromewell
  • Reveche, Ma.
  • Dilla-Ermita, Christine
  • Mauleon, Ramil
  • Leung, Hei
  • Bustamante, Carlos
  • McCouch, Susan
subjects:
  • Oryza sativa
  • Single nucleotide polymorphism
  • Illumina BeadXpress reader
ispartof: Molecular Breeding, 2012, Vol.29(4), pp.875-886
description: Multiplexed single nucleotide polymorphism (SNP) markers have the potential to increase the speed and cost-effectiveness of genotyping, provided that an optimal SNP density is used for each application. To test the efficiency of multiplexed SNP genotyping for diversity, mapping and breeding applications in rice ( Oryza sativa L.), we designed seven GoldenGate VeraCode oligo pool assay (OPA) sets for the Illumina BeadXpress Reader. Validated markers from existing 1536 Illumina SNPs and 44 K Affymetrix SNP chips developed at Cornell University were used to select subsets of informative SNPs for different germplasm groups with even distribution across the genome. A 96-plex OPA was developed for quality control purposes and for assigning a sample into one of the five O. sativa population subgroups. Six 384-plex OPAs were designed for genetic diversity analysis, DNA fingerprinting, and to have evenly-spaced polymorphic markers for quantitative trait locus (QTL) mapping and background selection for crosses between different germplasm pools in rice: Indica/Indica, Indica/Japonica, Japonica/Japonica, Indica/ O. rufipogon , and Japonica/ O. rufipogon . After testing on a diverse set of rice varieties, two of the SNP sets were re-designed by replacing poor-performing SNPs. Pilot studies were successfully performed for diversity analysis, QTL mapping, marker-assisted backcrossing, and developing specialized genetic stocks, demonstrating that 384-plex SNP genotyping on the BeadXpress platform is a robust and efficient method for marker genotyping in rice.
language: eng
source:
identifier: ISSN: 1380-3743 ; E-ISSN: 1572-9788 ; DOI: 10.1007/s11032-011-9663-x
fulltext: fulltext
issn:
  • 1572-9788
  • 15729788
  • 1380-3743
  • 13803743
url: Link


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titleHigh-throughput single nucleotide polymorphism genotyping for breeding applications in rice using the BeadXpress platform
creatorThomson, Michael ; Zhao, Keyan ; Wright, Mark ; McNally, Kenneth ; Rey, Jessica ; Tung, Chih-Wei ; Reynolds, Andy ; Scheffler, Brian ; Eizenga, Georgia ; McClung, Anna ; Kim, Hyunjung ; Ismail, Abdelbagi ; Ocampo, Marjorie ; Mojica, Chromewell ; Reveche, Ma. ; Dilla-Ermita, Christine ; Mauleon, Ramil ; Leung, Hei ; Bustamante, Carlos ; McCouch, Susan
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subjectOryza sativa ; Single nucleotide polymorphism ; Illumina BeadXpress reader
descriptionMultiplexed single nucleotide polymorphism (SNP) markers have the potential to increase the speed and cost-effectiveness of genotyping, provided that an optimal SNP density is used for each application. To test the efficiency of multiplexed SNP genotyping for diversity, mapping and breeding applications in rice ( Oryza sativa L.), we designed seven GoldenGate VeraCode oligo pool assay (OPA) sets for the Illumina BeadXpress Reader. Validated markers from existing 1536 Illumina SNPs and 44 K Affymetrix SNP chips developed at Cornell University were used to select subsets of informative SNPs for different germplasm groups with even distribution across the genome. A 96-plex OPA was developed for quality control purposes and for assigning a sample into one of the five O. sativa population subgroups. Six 384-plex OPAs were designed for genetic diversity analysis, DNA fingerprinting, and to have evenly-spaced polymorphic markers for quantitative trait locus (QTL) mapping and background selection for crosses between different germplasm pools in rice: Indica/Indica, Indica/Japonica, Japonica/Japonica, Indica/ O. rufipogon , and Japonica/ O. rufipogon . After testing on a diverse set of rice varieties, two of the SNP sets were re-designed by replacing poor-performing SNPs. Pilot studies were successfully performed for diversity analysis, QTL mapping, marker-assisted backcrossing, and developing specialized genetic stocks, demonstrating that 384-plex SNP genotyping on the BeadXpress platform is a robust and efficient method for marker genotyping in rice.
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titleHigh-throughput single nucleotide polymorphism genotyping for breeding applications in rice using the BeadXpress platform
descriptionMultiplexed single nucleotide polymorphism (SNP) markers have the potential to increase the speed and cost-effectiveness of genotyping, provided that an optimal SNP density is used for each application. To test the efficiency of multiplexed SNP genotyping for diversity, mapping and breeding applications in rice ( Oryza sativa L.), we designed seven GoldenGate VeraCode oligo pool assay (OPA) sets for the Illumina BeadXpress Reader. Validated markers from existing 1536 Illumina SNPs and 44 K Affymetrix SNP chips developed at Cornell University were used to select subsets of informative SNPs for different germplasm groups with even distribution across the genome. A 96-plex OPA was developed for quality control purposes and for assigning a sample into one of the five O. sativa population subgroups. Six 384-plex OPAs were designed for genetic diversity analysis, DNA fingerprinting, and to have evenly-spaced polymorphic markers for quantitative trait locus (QTL) mapping and background selection for crosses between different germplasm pools in rice: Indica/Indica, Indica/Japonica, Japonica/Japonica, Indica/ O. rufipogon , and Japonica/ O. rufipogon . After testing on a diverse set of rice varieties, two of the SNP sets were re-designed by replacing poor-performing SNPs. Pilot studies were successfully performed for diversity analysis, QTL mapping, marker-assisted backcrossing, and developing specialized genetic stocks, demonstrating that 384-plex SNP genotyping on the BeadXpress platform is a robust and efficient method for marker genotyping in rice.
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abstractMultiplexed single nucleotide polymorphism (SNP) markers have the potential to increase the speed and cost-effectiveness of genotyping, provided that an optimal SNP density is used for each application. To test the efficiency of multiplexed SNP genotyping for diversity, mapping and breeding applications in rice ( Oryza sativa L.), we designed seven GoldenGate VeraCode oligo pool assay (OPA) sets for the Illumina BeadXpress Reader. Validated markers from existing 1536 Illumina SNPs and 44 K Affymetrix SNP chips developed at Cornell University were used to select subsets of informative SNPs for different germplasm groups with even distribution across the genome. A 96-plex OPA was developed for quality control purposes and for assigning a sample into one of the five O. sativa population subgroups. Six 384-plex OPAs were designed for genetic diversity analysis, DNA fingerprinting, and to have evenly-spaced polymorphic markers for quantitative trait locus (QTL) mapping and background selection for crosses between different germplasm pools in rice: Indica/Indica, Indica/Japonica, Japonica/Japonica, Indica/ O. rufipogon , and Japonica/ O. rufipogon . After testing on a diverse set of rice varieties, two of the SNP sets were re-designed by replacing poor-performing SNPs. Pilot studies were successfully performed for diversity analysis, QTL mapping, marker-assisted backcrossing, and developing specialized genetic stocks, demonstrating that 384-plex SNP genotyping on the BeadXpress platform is a robust and efficient method for marker genotyping in rice.
copDordrecht
pubSpringer Netherlands
doi10.1007/s11032-011-9663-x
pages875-886
date2012-04