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Molecular marker-assisted backcrossing breeding: an example to transfer a thermostable β-amylase gene from wild barley

Molecular marker-assisted backcrossing (MABC) is widely recommended for transferring favorable alleles from a donor to an elite variety. The question remains whether MABC is an effective approach to developing a competitive commercial variety. Here, we illustrate the transfer of a thermostable β-amy... Full description

Journal Title: Molecular Breeding 2018, Vol.38(5), pp.1-9
Main Author: Xu, Yanhao
Other Authors: Zhang, Xiao-Qi , Harasymow, Stefan , Westcott, Sharon , Zhang, Wenying , Li, Chengdao
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1380-3743 ; E-ISSN: 1572-9788 ; DOI: 10.1007/s11032-018-0828-8
Link: http://dx.doi.org/10.1007/s11032-018-0828-8
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recordid: springer_jour10.1007/s11032-018-0828-8
title: Molecular marker-assisted backcrossing breeding: an example to transfer a thermostable β-amylase gene from wild barley
format: Article
creator:
  • Xu, Yanhao
  • Zhang, Xiao-Qi
  • Harasymow, Stefan
  • Westcott, Sharon
  • Zhang, Wenying
  • Li, Chengdao
subjects:
  • Barley
  • Thermostable β–amylase
  • Marker-assisted backcrossing
  • Conventional breeding
  • Hordeum spontaneum
ispartof: Molecular Breeding, 2018, Vol.38(5), pp.1-9
description: Molecular marker-assisted backcrossing (MABC) is widely recommended for transferring favorable alleles from a donor to an elite variety. The question remains whether MABC is an effective approach to developing a competitive commercial variety. Here, we illustrate the transfer of a thermostable β-amylase allele Sd3 from wild barley into a commercial barley variety Gairdner. The elite lines were chosen for the Regional Crop Variety Test that followed a standard conventional breeding process. The results demonstrated that the Sd3 allele not only increased enzyme thermostability but dramatically enhanced diastatic power, an important malting quality trait. The BC 1 F 1 individuals had a fundamental impact on the comprehensive agronomic and quality traits of the final progenies, demonstrating the importance of screening at the early stage of backcrossing in MABC. There was sufficient genetic variation in the BC 3 F 3 families to select other malting quality and agronomic traits. Ten individual breeding lines with improved β-amylase thermostability also had improved yields and grain plumpness. Three elite lines with improved malting quality and agronomic traits were selected to provide a parental line to incorporate the wild barley allele for breeding a commercial variety. A new strategy should be considered that uses marker-assisted selection and backcrossing to transfer a favorable allele from a wild parent.
language: eng
source:
identifier: ISSN: 1380-3743 ; E-ISSN: 1572-9788 ; DOI: 10.1007/s11032-018-0828-8
fulltext: fulltext
issn:
  • 1572-9788
  • 15729788
  • 1380-3743
  • 13803743
url: Link


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titleMolecular marker-assisted backcrossing breeding: an example to transfer a thermostable β-amylase gene from wild barley
creatorXu, Yanhao ; Zhang, Xiao-Qi ; Harasymow, Stefan ; Westcott, Sharon ; Zhang, Wenying ; Li, Chengdao
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subjectBarley ; Thermostable β–amylase ; Marker-assisted backcrossing ; Conventional breeding ; Hordeum spontaneum
descriptionMolecular marker-assisted backcrossing (MABC) is widely recommended for transferring favorable alleles from a donor to an elite variety. The question remains whether MABC is an effective approach to developing a competitive commercial variety. Here, we illustrate the transfer of a thermostable β-amylase allele Sd3 from wild barley into a commercial barley variety Gairdner. The elite lines were chosen for the Regional Crop Variety Test that followed a standard conventional breeding process. The results demonstrated that the Sd3 allele not only increased enzyme thermostability but dramatically enhanced diastatic power, an important malting quality trait. The BC 1 F 1 individuals had a fundamental impact on the comprehensive agronomic and quality traits of the final progenies, demonstrating the importance of screening at the early stage of backcrossing in MABC. There was sufficient genetic variation in the BC 3 F 3 families to select other malting quality and agronomic traits. Ten individual breeding lines with improved β-amylase thermostability also had improved yields and grain plumpness. Three elite lines with improved malting quality and agronomic traits were selected to provide a parental line to incorporate the wild barley allele for breeding a commercial variety. A new strategy should be considered that uses marker-assisted selection and backcrossing to transfer a favorable allele from a wild parent.
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descriptionMolecular marker-assisted backcrossing (MABC) is widely recommended for transferring favorable alleles from a donor to an elite variety. The question remains whether MABC is an effective approach to developing a competitive commercial variety. Here, we illustrate the transfer of a thermostable β-amylase allele Sd3 from wild barley into a commercial barley variety Gairdner. The elite lines were chosen for the Regional Crop Variety Test that followed a standard conventional breeding process. The results demonstrated that the Sd3 allele not only increased enzyme thermostability but dramatically enhanced diastatic power, an important malting quality trait. The BC 1 F 1 individuals had a fundamental impact on the comprehensive agronomic and quality traits of the final progenies, demonstrating the importance of screening at the early stage of backcrossing in MABC. There was sufficient genetic variation in the BC 3 F 3 families to select other malting quality and agronomic traits. Ten individual breeding lines with improved β-amylase thermostability also had improved yields and grain plumpness. Three elite lines with improved malting quality and agronomic traits were selected to provide a parental line to incorporate the wild barley allele for breeding a commercial variety. A new strategy should be considered that uses marker-assisted selection and backcrossing to transfer a favorable allele from a wild parent.
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abstractMolecular marker-assisted backcrossing (MABC) is widely recommended for transferring favorable alleles from a donor to an elite variety. The question remains whether MABC is an effective approach to developing a competitive commercial variety. Here, we illustrate the transfer of a thermostable β-amylase allele Sd3 from wild barley into a commercial barley variety Gairdner. The elite lines were chosen for the Regional Crop Variety Test that followed a standard conventional breeding process. The results demonstrated that the Sd3 allele not only increased enzyme thermostability but dramatically enhanced diastatic power, an important malting quality trait. The BC 1 F 1 individuals had a fundamental impact on the comprehensive agronomic and quality traits of the final progenies, demonstrating the importance of screening at the early stage of backcrossing in MABC. There was sufficient genetic variation in the BC 3 F 3 families to select other malting quality and agronomic traits. Ten individual breeding lines with improved β-amylase thermostability also had improved yields and grain plumpness. Three elite lines with improved malting quality and agronomic traits were selected to provide a parental line to incorporate the wild barley allele for breeding a commercial variety. A new strategy should be considered that uses marker-assisted selection and backcrossing to transfer a favorable allele from a wild parent.
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pubSpringer Netherlands
doi10.1007/s11032-018-0828-8
pages1-9
date2018-05