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Walleye dermal sarcoma virus: expression of a full-length clone or the rv - cyclin ( orf a ) gene is cytopathic to the host and human tumor cells

Walleye dermal sarcoma virus (WDSV) is etiologically associated with a skin tumor, walleye dermal sarcoma (WDS), which develops in the fall and regresses in the spring. WDSV genome contains, in addition to gag , pol and env , three open reading frames (orfs) designated orf a ( rv - cyclin ), orf b a... Full description

Journal Title: Molecular Biology Reports 2013, Vol.40(2), pp.1451-1461
Main Author: Xu, Kun
Other Authors: Zhang, Ting , Wang, Ling , Zhang, Cun , Zhang, Long , Ma, Li , Xin, Ying , Ren, Chong , Zhang, Zhi , Yan, Qiang , Martineau, Daniel , Zhang, Zhi
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0301-4851 ; E-ISSN: 1573-4978 ; DOI: 10.1007/s11033-012-2188-5
Link: http://dx.doi.org/10.1007/s11033-012-2188-5
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recordid: springer_jour10.1007/s11033-012-2188-5
title: Walleye dermal sarcoma virus: expression of a full-length clone or the rv - cyclin ( orf a ) gene is cytopathic to the host and human tumor cells
format: Article
creator:
  • Xu, Kun
  • Zhang, Ting
  • Wang, Ling
  • Zhang, Cun
  • Zhang, Long
  • Ma, Li
  • Xin, Ying
  • Ren, Chong
  • Zhang, Zhi
  • Yan, Qiang
  • Martineau, Daniel
  • Zhang, Zhi
subjects:
  • Walleye dermal sarcoma virus
  • Retroviral cyclin
  • Orf A
  • Tumor
ispartof: Molecular Biology Reports, 2013, Vol.40(2), pp.1451-1461
description: Walleye dermal sarcoma virus (WDSV) is etiologically associated with a skin tumor, walleye dermal sarcoma (WDS), which develops in the fall and regresses in the spring. WDSV genome contains, in addition to gag , pol and env , three open reading frames (orfs) designated orf a ( rv - cyclin ), orf b and orf c . Unintegrated linear WDSV provirus DNA isolated from infected tumor cells was used to construct a full-length WDSV provirus clone pWDSV, while orf a was cloned into pSVK3 to construct the expression vector porfA. Stable co-transfection of a walleye cell line (W12) with pWDSV and pcDNA3 generated fewer and smaller G418-resistant colonies compared to the control. By Northern blot analysis, several small transcripts (2.8, 1.8, 1.2, and 0.8 kb) were detected using a WDSV LTR-specific probe. By RT-PCR and Southern blot analysis, three cDNAs (2.4, 1.6 and 0.8 kb) were identified, including both orf a and orf b messenger. Furthermore stable co-transfection of both a human lung adenocarcinoma cell line (SPC-A-1) and a cervical cancer cell line (HeLa) with pcDNA3 and ether porfA or pWDSV also generated fewer and smaller G418-resistant colonies. We conclude that expression of the full-length WDSV clone or the orf a gene inhibits the host fish and human tumor cell growth, and Orf A protein maybe a potential factor which contributes to the seasonal tumor development and regression. This is the first fish provirus clone that has been expressed in cell culture system, which will provide a new in vitro model for tumor research and oncotherapy study.
language: eng
source:
identifier: ISSN: 0301-4851 ; E-ISSN: 1573-4978 ; DOI: 10.1007/s11033-012-2188-5
fulltext: fulltext
issn:
  • 1573-4978
  • 15734978
  • 0301-4851
  • 03014851
url: Link


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titleWalleye dermal sarcoma virus: expression of a full-length clone or the rv - cyclin ( orf a ) gene is cytopathic to the host and human tumor cells
creatorXu, Kun ; Zhang, Ting ; Wang, Ling ; Zhang, Cun ; Zhang, Long ; Ma, Li ; Xin, Ying ; Ren, Chong ; Zhang, Zhi ; Yan, Qiang ; Martineau, Daniel ; Zhang, Zhi
ispartofMolecular Biology Reports, 2013, Vol.40(2), pp.1451-1461
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subjectWalleye dermal sarcoma virus ; Retroviral cyclin ; Orf A ; Tumor
descriptionWalleye dermal sarcoma virus (WDSV) is etiologically associated with a skin tumor, walleye dermal sarcoma (WDS), which develops in the fall and regresses in the spring. WDSV genome contains, in addition to gag , pol and env , three open reading frames (orfs) designated orf a ( rv - cyclin ), orf b and orf c . Unintegrated linear WDSV provirus DNA isolated from infected tumor cells was used to construct a full-length WDSV provirus clone pWDSV, while orf a was cloned into pSVK3 to construct the expression vector porfA. Stable co-transfection of a walleye cell line (W12) with pWDSV and pcDNA3 generated fewer and smaller G418-resistant colonies compared to the control. By Northern blot analysis, several small transcripts (2.8, 1.8, 1.2, and 0.8 kb) were detected using a WDSV LTR-specific probe. By RT-PCR and Southern blot analysis, three cDNAs (2.4, 1.6 and 0.8 kb) were identified, including both orf a and orf b messenger. Furthermore stable co-transfection of both a human lung adenocarcinoma cell line (SPC-A-1) and a cervical cancer cell line (HeLa) with pcDNA3 and ether porfA or pWDSV also generated fewer and smaller G418-resistant colonies. We conclude that expression of the full-length WDSV clone or the orf a gene inhibits the host fish and human tumor cell growth, and Orf A protein maybe a potential factor which contributes to the seasonal tumor development and regression. This is the first fish provirus clone that has been expressed in cell culture system, which will provide a new in vitro model for tumor research and oncotherapy study.
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titleWalleye dermal sarcoma virus: expression of a full-length clone or the rv - cyclin ( orf a ) gene is cytopathic to the host and human tumor cells
descriptionWalleye dermal sarcoma virus (WDSV) is etiologically associated with a skin tumor, walleye dermal sarcoma (WDS), which develops in the fall and regresses in the spring. WDSV genome contains, in addition to gag , pol and env , three open reading frames (orfs) designated orf a ( rv - cyclin ), orf b and orf c . Unintegrated linear WDSV provirus DNA isolated from infected tumor cells was used to construct a full-length WDSV provirus clone pWDSV, while orf a was cloned into pSVK3 to construct the expression vector porfA. Stable co-transfection of a walleye cell line (W12) with pWDSV and pcDNA3 generated fewer and smaller G418-resistant colonies compared to the control. By Northern blot analysis, several small transcripts (2.8, 1.8, 1.2, and 0.8 kb) were detected using a WDSV LTR-specific probe. By RT-PCR and Southern blot analysis, three cDNAs (2.4, 1.6 and 0.8 kb) were identified, including both orf a and orf b messenger. Furthermore stable co-transfection of both a human lung adenocarcinoma cell line (SPC-A-1) and a cervical cancer cell line (HeLa) with pcDNA3 and ether porfA or pWDSV also generated fewer and smaller G418-resistant colonies. We conclude that expression of the full-length WDSV clone or the orf a gene inhibits the host fish and human tumor cell growth, and Orf A protein maybe a potential factor which contributes to the seasonal tumor development and regression. This is the first fish provirus clone that has been expressed in cell culture system, which will provide a new in vitro model for tumor research and oncotherapy study.
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abstractWalleye dermal sarcoma virus (WDSV) is etiologically associated with a skin tumor, walleye dermal sarcoma (WDS), which develops in the fall and regresses in the spring. WDSV genome contains, in addition to gag , pol and env , three open reading frames (orfs) designated orf a ( rv - cyclin ), orf b and orf c . Unintegrated linear WDSV provirus DNA isolated from infected tumor cells was used to construct a full-length WDSV provirus clone pWDSV, while orf a was cloned into pSVK3 to construct the expression vector porfA. Stable co-transfection of a walleye cell line (W12) with pWDSV and pcDNA3 generated fewer and smaller G418-resistant colonies compared to the control. By Northern blot analysis, several small transcripts (2.8, 1.8, 1.2, and 0.8 kb) were detected using a WDSV LTR-specific probe. By RT-PCR and Southern blot analysis, three cDNAs (2.4, 1.6 and 0.8 kb) were identified, including both orf a and orf b messenger. Furthermore stable co-transfection of both a human lung adenocarcinoma cell line (SPC-A-1) and a cervical cancer cell line (HeLa) with pcDNA3 and ether porfA or pWDSV also generated fewer and smaller G418-resistant colonies. We conclude that expression of the full-length WDSV clone or the orf a gene inhibits the host fish and human tumor cell growth, and Orf A protein maybe a potential factor which contributes to the seasonal tumor development and regression. This is the first fish provirus clone that has been expressed in cell culture system, which will provide a new in vitro model for tumor research and oncotherapy study.
copDordrecht
pubSpringer Netherlands
doi10.1007/s11033-012-2188-5
pages1451-1461
date2013-02