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Cloning and functional analysis of goat glucose transporter 4

Byline: Liqi Zhu (1), Qinghua Yu (1), Jian Lin (1), Qiang Zhang (1), Qian Yang (1) Keywords: Glucose transporters; Amino acid transporters; Glucose uptake; Mammary gland; mTOR Abstract: Objectives Glucose transporter (GLUT) 4 is a major mediator of blood glucose levels and a key regulator of whole-b... Full description

Journal Title: Molecular Biology Reports 2014, Vol.41(2), pp.757-768
Main Author: Zhu, Liqi
Other Authors: Yu, Qinghua , Lin, Jian , Zhang, Qiang , Yang, Qian
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0301-4851 ; E-ISSN: 1573-4978 ; DOI: 10.1007/s11033-013-2915-6
Link: http://dx.doi.org/10.1007/s11033-013-2915-6
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recordid: springer_jour10.1007/s11033-013-2915-6
title: Cloning and functional analysis of goat glucose transporter 4
format: Article
creator:
  • Zhu, Liqi
  • Yu, Qinghua
  • Lin, Jian
  • Zhang, Qiang
  • Yang, Qian
subjects:
  • Glucose transporters
  • Amino acid transporters
  • Glucose uptake
  • Mammary gland
  • mTOR
ispartof: Molecular Biology Reports, 2014, Vol.41(2), pp.757-768
description: Byline: Liqi Zhu (1), Qinghua Yu (1), Jian Lin (1), Qiang Zhang (1), Qian Yang (1) Keywords: Glucose transporters; Amino acid transporters; Glucose uptake; Mammary gland; mTOR Abstract: Objectives Glucose transporter (GLUT) 4 is a major mediator of blood glucose levels and a key regulator of whole-body glucose homeostasis. This study aimed at evaluating the function of goat GLUT4 on glucose absorption and the effect of GLUT4 on lactose synthesis in goat mammary gland epithelial (GMGE) cells. Methods Currently, the cDNA of GLUT4, a putative facilitative GLUT, was cloned from goat. To investigate the function of goat GLUT4, we constructed the eukaryotic expression vector pcDNA3.1-GLUT4 and used it to transfect GMGE cells, and then GLUT4 transfected GMGE (G4T-GMGE) cells were generated. The deduced GLUT4 sequence comprised 509 amino acids, what meant that a putative protein with a molecular weight of approximately 55 kDa would be produced. Both glucose uptake and lactose synthesis increased in the G4T-GMGE cells compared with the GMGE cells. At the transcriptional level, GLUT4 expression increased by nearly 55-fold in the G4T-GMGE cells, and the expression of amino acid transporters (SLC1A5, SLC3A2 and SLC7A5) enhanced as well in contrast, GLUT1 expression decreased by more than 50 % in the G4T-GMGE cells. Conclusion These results suggest that goat GLUT4 functions in the transport of glucose and it may play a positive role in amino acid uptake in mammary glands. Author Affiliation: (1) Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, Jiangsu, People's Republic of China Article History: Registration Date: 17/12/2013 Received Date: 12/12/2012 Accepted Date: 17/12/2013 Online Date: 01/01/2014
language: eng
source:
identifier: ISSN: 0301-4851 ; E-ISSN: 1573-4978 ; DOI: 10.1007/s11033-013-2915-6
fulltext: fulltext
issn:
  • 1573-4978
  • 15734978
  • 0301-4851
  • 03014851
url: Link


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descriptionByline: Liqi Zhu (1), Qinghua Yu (1), Jian Lin (1), Qiang Zhang (1), Qian Yang (1) Keywords: Glucose transporters; Amino acid transporters; Glucose uptake; Mammary gland; mTOR Abstract: Objectives Glucose transporter (GLUT) 4 is a major mediator of blood glucose levels and a key regulator of whole-body glucose homeostasis. This study aimed at evaluating the function of goat GLUT4 on glucose absorption and the effect of GLUT4 on lactose synthesis in goat mammary gland epithelial (GMGE) cells. Methods Currently, the cDNA of GLUT4, a putative facilitative GLUT, was cloned from goat. To investigate the function of goat GLUT4, we constructed the eukaryotic expression vector pcDNA3.1-GLUT4 and used it to transfect GMGE cells, and then GLUT4 transfected GMGE (G4T-GMGE) cells were generated. The deduced GLUT4 sequence comprised 509 amino acids, what meant that a putative protein with a molecular weight of approximately 55 kDa would be produced. Both glucose uptake and lactose synthesis increased in the G4T-GMGE cells compared with the GMGE cells. At the transcriptional level, GLUT4 expression increased by nearly 55-fold in the G4T-GMGE cells, and the expression of amino acid transporters (SLC1A5, SLC3A2 and SLC7A5) enhanced as well in contrast, GLUT1 expression decreased by more than 50 % in the G4T-GMGE cells. Conclusion These results suggest that goat GLUT4 functions in the transport of glucose and it may play a positive role in amino acid uptake in mammary glands. Author Affiliation: (1) Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, Jiangsu, People's Republic of China Article History: Registration Date: 17/12/2013 Received Date: 12/12/2012 Accepted Date: 17/12/2013 Online Date: 01/01/2014
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