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Synthesis and characterisation of highly fluorescent core–shell nanoparticles based on Alexa dyes

Current and future developments in the emerging field of nanobiotechnology are closely linked to the rational design of novel fluorescent nanomaterials, e.g. for biosensing and imaging applications. Here, the synthesis of bright near infrared (NIR)-emissive nanoparticles based on the grafting of sil... Full description

Journal Title: Journal of Nanoparticle Research 2012, Vol.14(2), pp.1-10
Main Author: Natte, Kishore
Other Authors: Behnke, Thomas , Orts-Gil, Guillermo , Würth, Christian , Friedrich, Jörg , Österle, Werner , Resch-Genger, Ute
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1388-0764 ; E-ISSN: 1572-896X ; DOI: 10.1007/s11051-011-0680-9
Link: http://dx.doi.org/10.1007/s11051-011-0680-9
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recordid: springer_jour10.1007/s11051-011-0680-9
title: Synthesis and characterisation of highly fluorescent core–shell nanoparticles based on Alexa dyes
format: Article
creator:
  • Natte, Kishore
  • Behnke, Thomas
  • Orts-Gil, Guillermo
  • Würth, Christian
  • Friedrich, Jörg
  • Österle, Werner
  • Resch-Genger, Ute
subjects:
  • Silica
  • Alexa dyes
  • Fluorescent particles
  • Quantum yields
  • Nanoparticles
  • Protective shell
  • Nanobiotechnology
ispartof: Journal of Nanoparticle Research, 2012, Vol.14(2), pp.1-10
description: Current and future developments in the emerging field of nanobiotechnology are closely linked to the rational design of novel fluorescent nanomaterials, e.g. for biosensing and imaging applications. Here, the synthesis of bright near infrared (NIR)-emissive nanoparticles based on the grafting of silica nanoparticles (SNPs) with 3-aminopropyl triethoxysilane (APTES) followed by covalent attachment of Alexa dyes and their subsequent shielding by an additional silica shell are presented. These nanoparticles were investigated by dynamic light scattering (DLS), transmission electron microscopy (TEM) and fluorescence spectroscopy. TEM studies revealed the monodispersity of the initially prepared and fluorophore-labelled silica particles and the subsequent formation of raspberry-like structures after addition of a silica precursor. Measurements of absolute fluorescence quantum yields of these scattering particle suspensions with an integrating sphere setup demonstrated the influence of dye labelling density-dependent fluorophore aggregation on the signaling behaviour of such nanoparticles.
language: eng
source:
identifier: ISSN: 1388-0764 ; E-ISSN: 1572-896X ; DOI: 10.1007/s11051-011-0680-9
fulltext: fulltext
issn:
  • 1572-896X
  • 1572896X
  • 1388-0764
  • 13880764
url: Link


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titleSynthesis and characterisation of highly fluorescent core–shell nanoparticles based on Alexa dyes
creatorNatte, Kishore ; Behnke, Thomas ; Orts-Gil, Guillermo ; Würth, Christian ; Friedrich, Jörg ; Österle, Werner ; Resch-Genger, Ute
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subjectSilica ; Alexa dyes ; Fluorescent particles ; Quantum yields ; Nanoparticles ; Protective shell ; Nanobiotechnology
descriptionCurrent and future developments in the emerging field of nanobiotechnology are closely linked to the rational design of novel fluorescent nanomaterials, e.g. for biosensing and imaging applications. Here, the synthesis of bright near infrared (NIR)-emissive nanoparticles based on the grafting of silica nanoparticles (SNPs) with 3-aminopropyl triethoxysilane (APTES) followed by covalent attachment of Alexa dyes and their subsequent shielding by an additional silica shell are presented. These nanoparticles were investigated by dynamic light scattering (DLS), transmission electron microscopy (TEM) and fluorescence spectroscopy. TEM studies revealed the monodispersity of the initially prepared and fluorophore-labelled silica particles and the subsequent formation of raspberry-like structures after addition of a silica precursor. Measurements of absolute fluorescence quantum yields of these scattering particle suspensions with an integrating sphere setup demonstrated the influence of dye labelling density-dependent fluorophore aggregation on the signaling behaviour of such nanoparticles.
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titleSynthesis and characterisation of highly fluorescent core–shell nanoparticles based on Alexa dyes
descriptionCurrent and future developments in the emerging field of nanobiotechnology are closely linked to the rational design of novel fluorescent nanomaterials, e.g. for biosensing and imaging applications. Here, the synthesis of bright near infrared (NIR)-emissive nanoparticles based on the grafting of silica nanoparticles (SNPs) with 3-aminopropyl triethoxysilane (APTES) followed by covalent attachment of Alexa dyes and their subsequent shielding by an additional silica shell are presented. These nanoparticles were investigated by dynamic light scattering (DLS), transmission electron microscopy (TEM) and fluorescence spectroscopy. TEM studies revealed the monodispersity of the initially prepared and fluorophore-labelled silica particles and the subsequent formation of raspberry-like structures after addition of a silica precursor. Measurements of absolute fluorescence quantum yields of these scattering particle suspensions with an integrating sphere setup demonstrated the influence of dye labelling density-dependent fluorophore aggregation on the signaling behaviour of such nanoparticles.
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abstractCurrent and future developments in the emerging field of nanobiotechnology are closely linked to the rational design of novel fluorescent nanomaterials, e.g. for biosensing and imaging applications. Here, the synthesis of bright near infrared (NIR)-emissive nanoparticles based on the grafting of silica nanoparticles (SNPs) with 3-aminopropyl triethoxysilane (APTES) followed by covalent attachment of Alexa dyes and their subsequent shielding by an additional silica shell are presented. These nanoparticles were investigated by dynamic light scattering (DLS), transmission electron microscopy (TEM) and fluorescence spectroscopy. TEM studies revealed the monodispersity of the initially prepared and fluorophore-labelled silica particles and the subsequent formation of raspberry-like structures after addition of a silica precursor. Measurements of absolute fluorescence quantum yields of these scattering particle suspensions with an integrating sphere setup demonstrated the influence of dye labelling density-dependent fluorophore aggregation on the signaling behaviour of such nanoparticles.
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doi10.1007/s11051-011-0680-9
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