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Lack of Apparent Neurological Abnormalities in Rabbits Sensitized by Gangliosides

Two very high titer polyclonal antibodies against two ganglioside antigens, GM1 and GD1a, have been raised in New Zealand white rabbits using a homogeneous suspension of the highly purified antigens in Keyhole Limpet Hemocyanin and Freund’s adjuvant. The antisera were prepared over a period of 6 mon... Full description

Journal Title: Neurochemical Research 2004, Vol.29(11), pp.2147-2152
Main Author: Dasgupta, Somsankar
Other Authors: Li, Donna , Yu, Robert
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Springer Science & Business Media B.V.
ID: ISSN: 0364-3190 ; E-ISSN: 1573-6903 ; DOI: 10.1007/s11064-004-6888-7
Link: http://dx.doi.org/10.1007/s11064-004-6888-7
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recordid: springer_jour10.1007/s11064-004-6888-7
title: Lack of Apparent Neurological Abnormalities in Rabbits Sensitized by Gangliosides
format: Article
creator:
  • Dasgupta, Somsankar
  • Li, Donna
  • Yu, Robert
subjects:
  • Antibody
  • autoimmunity
  • Glycosphingolipid
  • neurological disorders
ispartof: Neurochemical Research, 2004, Vol.29(11), pp.2147-2152
description: Two very high titer polyclonal antibodies against two ganglioside antigens, GM1 and GD1a, have been raised in New Zealand white rabbits using a homogeneous suspension of the highly purified antigens in Keyhole Limpet Hemocyanin and Freund’s adjuvant. The antisera were prepared over a period of 6 months with repeated injections of the ganglioside suspension, followed by an intravenous injection of the purified ganglioside solution, and collecting the serum (approximately 50 ml) at defined time intervals. The GM1-antibody, thus prepared, showed a cross reactivity toward GD1b and asialo-GM1 (GA1), while the GD1a-antibody reacted with GD1a, GM1 and GA1 and GD1b as determined by immuno-overlay and ELISA methods. The titer for GM1 antiserum, determined by ELISA, was greater than 1/10,000 dilution while the titer for GD1a antibody was greater than 1/5,000 dilution. No neurological or behavioral abnormality was observed during the period of antiserum production. To evaluate any likely pathological damage caused by such a high titer ganglioside-antibody, autopsy of CNS as well PNS tissues from the rabbits were carried out after the final bleeding. No obvious pathological changes, including demyelination, were noted in any of the four rabbits. These observations cast doubt as to the direct effect of anti-ganglioside antibody induced neurological and pathological disorders.
language: eng
source: Springer Science & Business Media B.V.
identifier: ISSN: 0364-3190 ; E-ISSN: 1573-6903 ; DOI: 10.1007/s11064-004-6888-7
fulltext: fulltext
issn:
  • 1573-6903
  • 15736903
  • 0364-3190
  • 03643190
url: Link


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titleLack of Apparent Neurological Abnormalities in Rabbits Sensitized by Gangliosides
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subjectAntibody ; autoimmunity ; Glycosphingolipid ; neurological disorders
descriptionTwo very high titer polyclonal antibodies against two ganglioside antigens, GM1 and GD1a, have been raised in New Zealand white rabbits using a homogeneous suspension of the highly purified antigens in Keyhole Limpet Hemocyanin and Freund’s adjuvant. The antisera were prepared over a period of 6 months with repeated injections of the ganglioside suspension, followed by an intravenous injection of the purified ganglioside solution, and collecting the serum (approximately 50 ml) at defined time intervals. The GM1-antibody, thus prepared, showed a cross reactivity toward GD1b and asialo-GM1 (GA1), while the GD1a-antibody reacted with GD1a, GM1 and GA1 and GD1b as determined by immuno-overlay and ELISA methods. The titer for GM1 antiserum, determined by ELISA, was greater than 1/10,000 dilution while the titer for GD1a antibody was greater than 1/5,000 dilution. No neurological or behavioral abnormality was observed during the period of antiserum production. To evaluate any likely pathological damage caused by such a high titer ganglioside-antibody, autopsy of CNS as well PNS tissues from the rabbits were carried out after the final bleeding. No obvious pathological changes, including demyelination, were noted in any of the four rabbits. These observations cast doubt as to the direct effect of anti-ganglioside antibody induced neurological and pathological disorders.
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abstractTwo very high titer polyclonal antibodies against two ganglioside antigens, GM1 and GD1a, have been raised in New Zealand white rabbits using a homogeneous suspension of the highly purified antigens in Keyhole Limpet Hemocyanin and Freund’s adjuvant. The antisera were prepared over a period of 6 months with repeated injections of the ganglioside suspension, followed by an intravenous injection of the purified ganglioside solution, and collecting the serum (approximately 50 ml) at defined time intervals. The GM1-antibody, thus prepared, showed a cross reactivity toward GD1b and asialo-GM1 (GA1), while the GD1a-antibody reacted with GD1a, GM1 and GA1 and GD1b as determined by immuno-overlay and ELISA methods. The titer for GM1 antiserum, determined by ELISA, was greater than 1/10,000 dilution while the titer for GD1a antibody was greater than 1/5,000 dilution. No neurological or behavioral abnormality was observed during the period of antiserum production. To evaluate any likely pathological damage caused by such a high titer ganglioside-antibody, autopsy of CNS as well PNS tissues from the rabbits were carried out after the final bleeding. No obvious pathological changes, including demyelination, were noted in any of the four rabbits. These observations cast doubt as to the direct effect of anti-ganglioside antibody induced neurological and pathological disorders.
copNew York
pubKluwer Academic Publishers-Plenum Publishers
doi10.1007/s11064-004-6888-7
date2004-11