schliessen

Filtern

 

Bibliotheken

Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR

The outbreak of a novel influenza A (H1N1) virus across the globe poses a threat to human health. It is of paramount importance to develop a rapid, reliable and inexpensive diagnostic procedure. Based on the bioinformatic information from public database, primers specific for influenza A virus surfa... Full description

Journal Title: Chinese Science Bulletin 2009, Vol.54(13), pp.2164-2167
Main Author: Yan, An
Other Authors: Ding, GuoHui , Zhou, ZhenFeng , Dong, Hui , Zhang, YaKun , Zhu, Lei , He, YunGang , Zhang, GuoQing , Li, YiXue , Sun, Bing , Huang, Zhong , Lan, Ke , Jin, Li , Wang, HongYan , Wang, XiaoNing , Yang, Zhong , Zhong, Yang , Dai, JianXin , Guo, YaJun , Wang, Hao , Che, XiaoYan , Wu, Fan , Yuan, ZhenGan , Zhang, Xi , Cao, ZhiWei , Zhou, XiaoNong , Zhou, JiaHai , Ma, ZhiYong , Tong, GuangZhi , Zhao, GuoPing , Jin, WeiRong , Xiong, Hui
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1001-6538 ; E-ISSN: 1861-9541 ; DOI: 10.1007/s11434-009-0433-7
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: springer_jour10.1007/s11434-009-0433-7
title: Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR
format: Article
creator:
  • Yan, An
  • Ding, GuoHui
  • Zhou, ZhenFeng
  • Dong, Hui
  • Zhang, YaKun
  • Zhu, Lei
  • He, YunGang
  • Zhang, GuoQing
  • Li, YiXue
  • Sun, Bing
  • Huang, Zhong
  • Lan, Ke
  • Jin, Li
  • Wang, HongYan
  • Wang, XiaoNing
  • Yang, Zhong
  • Zhong, Yang
  • Dai, JianXin
  • Guo, YaJun
  • Wang, Hao
  • Che, XiaoYan
  • Wu, Fan
  • Yuan, ZhenGan
  • Zhang, Xi
  • Cao, ZhiWei
  • Zhou, XiaoNong
  • Zhou, JiaHai
  • Ma, ZhiYong
  • Tong, GuangZhi
  • Zhao, GuoPing
  • Jin, WeiRong
  • Xiong, Hui
subjects:
  • influenza A virus
  • gene
  • H1 and H3 subtypes
  • specific amplification by RT-PCT
  • DNA sequencing
ispartof: Chinese Science Bulletin, 2009, Vol.54(13), pp.2164-2167
description: The outbreak of a novel influenza A (H1N1) virus across the globe poses a threat to human health. It is of paramount importance to develop a rapid, reliable and inexpensive diagnostic procedure. Based on the bioinformatic information from public database, primers specific for influenza A virus surface protein haemagglutinin (HA) of several subtypes (including H1, H2, H3, H5, H7 and H9) were designed. Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others. This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1, but also provide the strategies to monitor other new types of influenza virus with explosive potential.
language: eng
source:
identifier: ISSN: 1001-6538 ; E-ISSN: 1861-9541 ; DOI: 10.1007/s11434-009-0433-7
fulltext: fulltext_linktorsrc
issn:
  • 1861-9541
  • 18619541
  • 1001-6538
  • 10016538
url: Link


@attributes
ID1591854483
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.1007/s11434-009-0433-7
sourceidspringer_jour
recordidTN_springer_jour10.1007/s11434-009-0433-7
sourcesystemPC
pqid20148374
display
typearticle
titleSubtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR
creatorYan, An ; Ding, GuoHui ; Zhou, ZhenFeng ; Dong, Hui ; Zhang, YaKun ; Zhu, Lei ; He, YunGang ; Zhang, GuoQing ; Li, YiXue ; Sun, Bing ; Huang, Zhong ; Lan, Ke ; Jin, Li ; Wang, HongYan ; Wang, XiaoNing ; Yang, Zhong ; Zhong, Yang ; Dai, JianXin ; Guo, YaJun ; Wang, Hao ; Che, XiaoYan ; Wu, Fan ; Yuan, ZhenGan ; Zhang, Xi ; Cao, ZhiWei ; Zhou, XiaoNong ; Zhou, JiaHai ; Ma, ZhiYong ; Tong, GuangZhi ; Zhao, GuoPing ; Jin, WeiRong ; Xiong, Hui
ispartofChinese Science Bulletin, 2009, Vol.54(13), pp.2164-2167
identifier
subjectinfluenza A virus ; gene ; H1 and H3 subtypes ; specific amplification by RT-PCT ; DNA sequencing
descriptionThe outbreak of a novel influenza A (H1N1) virus across the globe poses a threat to human health. It is of paramount importance to develop a rapid, reliable and inexpensive diagnostic procedure. Based on the bioinformatic information from public database, primers specific for influenza A virus surface protein haemagglutinin (HA) of several subtypes (including H1, H2, H3, H5, H7 and H9) were designed. Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others. This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1, but also provide the strategies to monitor other new types of influenza virus with explosive potential.
languageeng
source
version4
oafree_for_read
lds50peer_reviewed
links
openurl$$Topenurl_article
linktorsrc$$Uhttp://dx.doi.org/10.1007/s11434-009-0433-7$$EView_full_text_in_Springer
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Yan, An
1Ding, GuoHui
2Zhou, ZhenFeng
3Dong, Hui
4Zhang, YaKun
5Zhu, Lei
6He, YunGang
7Zhang, GuoQing
8Li, YiXue
9Sun, Bing
10Huang, Zhong
11Lan, Ke
12Jin, Li
13Wang, HongYan
14Wang, XiaoNing
15Yang, Zhong
16Zhong, Yang
17Dai, JianXin
18Guo, YaJun
19Wang, Hao
20Che, XiaoYan
21Wu, Fan
22Yuan, ZhenGan
23Zhang, Xi
24Cao, ZhiWei
25Zhou, XiaoNong
26Zhou, JiaHai
27Ma, ZhiYong
28Tong, GuangZhi
29Zhao, GuoPing
30Jin, WeiRong
31Xiong, Hui
titleSubtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR
descriptionThe outbreak of a novel influenza A (H1N1) virus across the globe poses a threat to human health. It is of paramount importance to develop a rapid, reliable and inexpensive diagnostic procedure. Based on the bioinformatic information from public database, primers specific for influenza A virus surface protein haemagglutinin (HA) of several subtypes (including H1, H2, H3, H5, H7 and H9) were designed. Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others. This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1, but also provide the strategies to monitor other new types of influenza virus with explosive potential.
subject
0influenza A virus
1gene
2H1 and H3 subtypes
3specific amplification by RT-PCT
4DNA sequencing
general
010.1007/s11434-009-0433-7
1English
2Springer Science & Business Media B.V.
3SpringerLink Open Access
sourceidspringer_jour
recordidspringer_jour10.1007/s11434-009-0433-7
issn
01861-9541
118619541
21001-6538
310016538
rsrctypearticle
creationdate2009
addtitle
0Chinese Science Bulletin
1Chin. Sci. Bull.
searchscopespringer_free
scopespringer_free
lsr30VSR-Enriched:[pqid, pages]
sort
titleSubtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR
authorYan, An ; Ding, GuoHui ; Zhou, ZhenFeng ; Dong, Hui ; Zhang, YaKun ; Zhu, Lei ; He, YunGang ; Zhang, GuoQing ; Li, YiXue ; Sun, Bing ; Huang, Zhong ; Lan, Ke ; Jin, Li ; Wang, HongYan ; Wang, XiaoNing ; Yang, Zhong ; Zhong, Yang ; Dai, JianXin ; Guo, YaJun ; Wang, Hao ; Che, XiaoYan ; Wu, Fan ; Yuan, ZhenGan ; Zhang, Xi ; Cao, ZhiWei ; Zhou, XiaoNong ; Zhou, JiaHai ; Ma, ZhiYong ; Tong, GuangZhi ; Zhao, GuoPing ; Jin, WeiRong ; Xiong, Hui
creationdate20090700
facets
frbrgroupid8780467705505211935
frbrtype5
languageeng
creationdate2009
topic
0Influenza A Virus
1Gene
2H1 And H3 Subtypes
3Specific Amplification By Rt-Pct
4Dna Sequencing
collectionSpringerLink Open Access
prefilterarticles
rsrctypearticles
creatorcontrib
0Yan, An
1Ding, GuoHui
2Zhou, ZhenFeng
3Dong, Hui
4Zhang, YaKun
5Zhu, Lei
6He, YunGang
7Zhang, GuoQing
8Li, YiXue
9Sun, Bing
10Huang, Zhong
11Lan, Ke
12Jin, Li
13Wang, HongYan
14Wang, XiaoNing
15Yang, Zhong
16Zhong, Yang
17Dai, JianXin
18Guo, YaJun
19Wang, Hao
20Che, XiaoYan
21Wu, Fan
22Yuan, ZhenGan
23Zhang, Xi
24Cao, ZhiWei
25Zhou, XiaoNong
26Zhou, JiaHai
27Ma, ZhiYong
28Tong, GuangZhi
29Zhao, GuoPing
30Jin, WeiRong
31Xiong, Hui
jtitleChinese Science Bulletin
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext_linktorsrc
addata
aulast
0Yan
1Ding
2Zhou
3Dong
4Zhang
5Zhu
6He
7Li
8Sun
9Huang
10Lan
11Jin
12Wang
13Yang
14Zhong
15Dai
16Guo
17Che
18Wu
19Yuan
20Cao
21Ma
22Tong
23Zhao
24Xiong
aufirst
0An
1GuoHui
2ZhenFeng
3Hui
4YaKun
5Lei
6YunGang
7GuoQing
8YiXue
9Bing
10Zhong
11Ke
12Li
13HongYan
14XiaoNing
15Yang
16JianXin
17YaJun
18Hao
19XiaoYan
20Fan
21ZhenGan
22Xi
23ZhiWei
24XiaoNong
25JiaHai
26ZhiYong
27GuangZhi
28GuoPing
29WeiRong
au
0Yan, An
1Ding, GuoHui
2Zhou, ZhenFeng
3Dong, Hui
4Zhang, YaKun
5Zhu, Lei
6He, YunGang
7Zhang, GuoQing
8Li, YiXue
9Sun, Bing
10Huang, Zhong
11Lan, Ke
12Jin, Li
13Wang, HongYan
14Wang, XiaoNing
15Yang, Zhong
16Zhong, Yang
17Dai, JianXin
18Guo, YaJun
19Wang, Hao
20Che, XiaoYan
21Wu, Fan
22Yuan, ZhenGan
23Zhang, Xi
24Cao, ZhiWei
25Zhou, XiaoNong
26Zhou, JiaHai
27Ma, ZhiYong
28Tong, GuangZhi
29Zhao, GuoPing
30Jin, WeiRong
31Xiong, Hui
atitleSubtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR
jtitleChinese Science Bulletin
stitleChin. Sci. Bull.
risdate200907
volume54
issue13
spage2164
epage2167
issn1001-6538
eissn1861-9541
genrearticle
ristypeJOUR
abstractThe outbreak of a novel influenza A (H1N1) virus across the globe poses a threat to human health. It is of paramount importance to develop a rapid, reliable and inexpensive diagnostic procedure. Based on the bioinformatic information from public database, primers specific for influenza A virus surface protein haemagglutinin (HA) of several subtypes (including H1, H2, H3, H5, H7 and H9) were designed. Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others. This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1, but also provide the strategies to monitor other new types of influenza virus with explosive potential.
copHeidelberg
pubSP Science in China Press
doi10.1007/s11434-009-0433-7
pages2164-2167
oafree_for_read
date2009-07