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Experimental study on the cryopreservation of LLC-PK1 epithelial cells with hypoxic UW solution

The effects of oxygen partial pressure on cryopreservation of the cells with organ preservation solution were explored. Hypoxic UW solution was made by purging the UW solution with argon. The pig proximal tubule epithelial cells (LLC-PK1 cells) were cryopreserved in hypoxic UW solution (Ar-UW group)... Full description

Journal Title: Journal of Huazhong University of Science and Technology 2007, Vol.27(4), pp.426-428
Main Author: Wan, Chidan
Other Authors: Wang, Chunyou , Liu, Tao , Wang, Hongbo , Yang, Zhiyong
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1672-0733 ; E-ISSN: 1993-1352 ; DOI: 10.1007/s11596-007-0419-0
Link: http://dx.doi.org/10.1007/s11596-007-0419-0
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recordid: springer_jour10.1007/s11596-007-0419-0
title: Experimental study on the cryopreservation of LLC-PK1 epithelial cells with hypoxic UW solution
format: Article
creator:
  • Wan, Chidan
  • Wang, Chunyou
  • Liu, Tao
  • Wang, Hongbo
  • Yang, Zhiyong
subjects:
  • organ preservation solution
  • oxygen partial pressure
  • cryopreservation
ispartof: Journal of Huazhong University of Science and Technology, 2007, Vol.27(4), pp.426-428
description: The effects of oxygen partial pressure on cryopreservation of the cells with organ preservation solution were explored. Hypoxic UW solution was made by purging the UW solution with argon. The pig proximal tubule epithelial cells (LLC-PK1 cells) were cryopreserved in hypoxic UW solution (Ar-UW group) or standard UW solution (UW group) at 4°C for 48 h. Trypan blue staining and LDH detection were performed to evaluate the injury of the cells. The results showed that the oxygen partial pressure in Ar-UW group was significantly declined from 242±6 mmHg to 83±10 mmHg. After cryopreservation at 4°C for 48 h, LDH leakage rate and Trypan blue-stained rate in Ar-UW group were (11.3±3.4)% and (10.5±4.7)%, respectively, which were significantly lower than in UW group [(49.5±6.9)% and (47.6±9.3)% respectively, both P
language: eng
source:
identifier: ISSN: 1672-0733 ; E-ISSN: 1993-1352 ; DOI: 10.1007/s11596-007-0419-0
fulltext: fulltext
issn:
  • 1993-1352
  • 19931352
  • 1672-0733
  • 16720733
url: Link


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titleExperimental study on the cryopreservation of LLC-PK1 epithelial cells with hypoxic UW solution
creatorWan, Chidan ; Wang, Chunyou ; Liu, Tao ; Wang, Hongbo ; Yang, Zhiyong
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subjectorgan preservation solution ; oxygen partial pressure ; cryopreservation
descriptionThe effects of oxygen partial pressure on cryopreservation of the cells with organ preservation solution were explored. Hypoxic UW solution was made by purging the UW solution with argon. The pig proximal tubule epithelial cells (LLC-PK1 cells) were cryopreserved in hypoxic UW solution (Ar-UW group) or standard UW solution (UW group) at 4°C for 48 h. Trypan blue staining and LDH detection were performed to evaluate the injury of the cells. The results showed that the oxygen partial pressure in Ar-UW group was significantly declined from 242±6 mmHg to 83±10 mmHg. After cryopreservation at 4°C for 48 h, LDH leakage rate and Trypan blue-stained rate in Ar-UW group were (11.3±3.4)% and (10.5±4.7)%, respectively, which were significantly lower than in UW group [(49.5±6.9)% and (47.6±9.3)% respectively, both P <0.01]. It was concluded that lower oxygen partial pressure of UW solution was more beneficial to the cryopreservation of LLC.
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titleExperimental study on the cryopreservation of LLC-PK1 epithelial cells with hypoxic UW solution
descriptionThe effects of oxygen partial pressure on cryopreservation of the cells with organ preservation solution were explored. Hypoxic UW solution was made by purging the UW solution with argon. The pig proximal tubule epithelial cells (LLC-PK1 cells) were cryopreserved in hypoxic UW solution (Ar-UW group) or standard UW solution (UW group) at 4°C for 48 h. Trypan blue staining and LDH detection were performed to evaluate the injury of the cells. The results showed that the oxygen partial pressure in Ar-UW group was significantly declined from 242±6 mmHg to 83±10 mmHg. After cryopreservation at 4°C for 48 h, LDH leakage rate and Trypan blue-stained rate in Ar-UW group were (11.3±3.4)% and (10.5±4.7)%, respectively, which were significantly lower than in UW group [(49.5±6.9)% and (47.6±9.3)% respectively, both P <0.01]. It was concluded that lower oxygen partial pressure of UW solution was more beneficial to the cryopreservation of LLC.
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abstractThe effects of oxygen partial pressure on cryopreservation of the cells with organ preservation solution were explored. Hypoxic UW solution was made by purging the UW solution with argon. The pig proximal tubule epithelial cells (LLC-PK1 cells) were cryopreserved in hypoxic UW solution (Ar-UW group) or standard UW solution (UW group) at 4°C for 48 h. Trypan blue staining and LDH detection were performed to evaluate the injury of the cells. The results showed that the oxygen partial pressure in Ar-UW group was significantly declined from 242±6 mmHg to 83±10 mmHg. After cryopreservation at 4°C for 48 h, LDH leakage rate and Trypan blue-stained rate in Ar-UW group were (11.3±3.4)% and (10.5±4.7)%, respectively, which were significantly lower than in UW group [(49.5±6.9)% and (47.6±9.3)% respectively, both P <0.01]. It was concluded that lower oxygen partial pressure of UW solution was more beneficial to the cryopreservation of LLC.
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