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Inhibition of calpain on oxygen glucose deprivation-induced RGC-5 necroptosis

The purpose of this study was to investigate the effect of inhibition of calpain on retinal ganglion cell-5 (RGC-5) necroptosis following oxygen glucose deprivation (OGD). RGC-5 cells were cultured in Dulbecco’s-modified essential medium and necroptosis was induced by 8-h OGD. PI staining and flow c... Full description

Journal Title: Journal of Huazhong University of Science and Technology [Medical Sciences] 2016, Vol.36(5), pp.639-645
Main Author: Chen, Shuang
Other Authors: Yan, Jie , Deng, Hai-xiao , Long, Ling-ling , Hu, Yong-jun , Wang, Mi , Shang, Lei , Chen, Dan , Huang, Ju-fang , Xiong, Kun
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1672-0733 ; E-ISSN: 1993-1352 ; DOI: 10.1007/s11596-016-1639-y
Link: http://dx.doi.org/10.1007/s11596-016-1639-y
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recordid: springer_jour10.1007/s11596-016-1639-y
title: Inhibition of calpain on oxygen glucose deprivation-induced RGC-5 necroptosis
format: Article
creator:
  • Chen, Shuang
  • Yan, Jie
  • Deng, Hai-xiao
  • Long, Ling-ling
  • Hu, Yong-jun
  • Wang, Mi
  • Shang, Lei
  • Chen, Dan
  • Huang, Ju-fang
  • Xiong, Kun
subjects:
  • oxygen glucose deprivation
  • necroptosis
  • calpain
  • retinal ganglion cell-5
  • N-acetyl-leucyl-leucyl-norleucinal
  • truncated apoptosis inducing factor
ispartof: Journal of Huazhong University of Science and Technology [Medical Sciences], 2016, Vol.36(5), pp.639-645
description: The purpose of this study was to investigate the effect of inhibition of calpain on retinal ganglion cell-5 (RGC-5) necroptosis following oxygen glucose deprivation (OGD). RGC-5 cells were cultured in Dulbecco’s-modified essential medium and necroptosis was induced by 8-h OGD. PI staining and flow cytometry were performed to detect RGC-5 necrosis. The calpain expression was detected by Western blotting and immunofluorescence staining. The calpain activity was tested by activity detection kit. Flow cytometry was used to detect the effect of calpain on RGC-5 necroptosis following OGD with or without N-acetyl-leucyl-leucyl-norleucinal (ALLN) pre-treatment. Western blot was used to detect the protein level of truncated apoptosis inducing factor (tAIF) in RGC-5 cells following OGD. The results showed that there was an up-regulation of the calpain expression and activity following OGD. Upon adding ALLN, the calpain activity was inhibited and tAIF was reduced following OGD along with the decreased number of RGC-5 necroptosis. In conclusion, calpain was involved in OGD-induced RGC-5 necroptosis with the increased expression of its downstream molecule tAIF.
language: eng
source:
identifier: ISSN: 1672-0733 ; E-ISSN: 1993-1352 ; DOI: 10.1007/s11596-016-1639-y
fulltext: fulltext
issn:
  • 1993-1352
  • 19931352
  • 1672-0733
  • 16720733
url: Link


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titleInhibition of calpain on oxygen glucose deprivation-induced RGC-5 necroptosis
creatorChen, Shuang ; Yan, Jie ; Deng, Hai-xiao ; Long, Ling-ling ; Hu, Yong-jun ; Wang, Mi ; Shang, Lei ; Chen, Dan ; Huang, Ju-fang ; Xiong, Kun
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subjectoxygen glucose deprivation ; necroptosis ; calpain ; retinal ganglion cell-5 ; N-acetyl-leucyl-leucyl-norleucinal ; truncated apoptosis inducing factor
descriptionThe purpose of this study was to investigate the effect of inhibition of calpain on retinal ganglion cell-5 (RGC-5) necroptosis following oxygen glucose deprivation (OGD). RGC-5 cells were cultured in Dulbecco’s-modified essential medium and necroptosis was induced by 8-h OGD. PI staining and flow cytometry were performed to detect RGC-5 necrosis. The calpain expression was detected by Western blotting and immunofluorescence staining. The calpain activity was tested by activity detection kit. Flow cytometry was used to detect the effect of calpain on RGC-5 necroptosis following OGD with or without N-acetyl-leucyl-leucyl-norleucinal (ALLN) pre-treatment. Western blot was used to detect the protein level of truncated apoptosis inducing factor (tAIF) in RGC-5 cells following OGD. The results showed that there was an up-regulation of the calpain expression and activity following OGD. Upon adding ALLN, the calpain activity was inhibited and tAIF was reduced following OGD along with the decreased number of RGC-5 necroptosis. In conclusion, calpain was involved in OGD-induced RGC-5 necroptosis with the increased expression of its downstream molecule tAIF.
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titleInhibition of calpain on oxygen glucose deprivation-induced RGC-5 necroptosis
descriptionThe purpose of this study was to investigate the effect of inhibition of calpain on retinal ganglion cell-5 (RGC-5) necroptosis following oxygen glucose deprivation (OGD). RGC-5 cells were cultured in Dulbecco’s-modified essential medium and necroptosis was induced by 8-h OGD. PI staining and flow cytometry were performed to detect RGC-5 necrosis. The calpain expression was detected by Western blotting and immunofluorescence staining. The calpain activity was tested by activity detection kit. Flow cytometry was used to detect the effect of calpain on RGC-5 necroptosis following OGD with or without N-acetyl-leucyl-leucyl-norleucinal (ALLN) pre-treatment. Western blot was used to detect the protein level of truncated apoptosis inducing factor (tAIF) in RGC-5 cells following OGD. The results showed that there was an up-regulation of the calpain expression and activity following OGD. Upon adding ALLN, the calpain activity was inhibited and tAIF was reduced following OGD along with the decreased number of RGC-5 necroptosis. In conclusion, calpain was involved in OGD-induced RGC-5 necroptosis with the increased expression of its downstream molecule tAIF.
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abstractThe purpose of this study was to investigate the effect of inhibition of calpain on retinal ganglion cell-5 (RGC-5) necroptosis following oxygen glucose deprivation (OGD). RGC-5 cells were cultured in Dulbecco’s-modified essential medium and necroptosis was induced by 8-h OGD. PI staining and flow cytometry were performed to detect RGC-5 necrosis. The calpain expression was detected by Western blotting and immunofluorescence staining. The calpain activity was tested by activity detection kit. Flow cytometry was used to detect the effect of calpain on RGC-5 necroptosis following OGD with or without N-acetyl-leucyl-leucyl-norleucinal (ALLN) pre-treatment. Western blot was used to detect the protein level of truncated apoptosis inducing factor (tAIF) in RGC-5 cells following OGD. The results showed that there was an up-regulation of the calpain expression and activity following OGD. Upon adding ALLN, the calpain activity was inhibited and tAIF was reduced following OGD along with the decreased number of RGC-5 necroptosis. In conclusion, calpain was involved in OGD-induced RGC-5 necroptosis with the increased expression of its downstream molecule tAIF.
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doi10.1007/s11596-016-1639-y
pages639-645
date2016-10