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Molecular mechanism of elicitor-induced tanshinone accumulation in Salvia miltiorrhiza hairy root cultures

To develop an optimal bioprocess for the production of tanshinone which is mainly used for the treatment of cardiocerebral vascular disease, the tanshinone biosynthetic pathway regulation must be better understood. In this paper, expression of tanshinone biosynthetic pathway related genes as well as... Full description

Journal Title: Acta Physiologiae Plantarum 2012, Vol.34(4), pp.1421-1433
Main Author: Kai, Guoyin
Other Authors: Liao, Pan , Xu, Hui , Wang, Jing , Zhou, Congcong , Zhou, Wei , Qi, Yaping , Xiao, Jianbo , Wang, Yuliang , Zhang, Lin
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0137-5881 ; E-ISSN: 1861-1664 ; DOI: 10.1007/s11738-012-0940-z
Link: http://dx.doi.org/10.1007/s11738-012-0940-z
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recordid: springer_jour10.1007/s11738-012-0940-z
title: Molecular mechanism of elicitor-induced tanshinone accumulation in Salvia miltiorrhiza hairy root cultures
format: Article
creator:
  • Kai, Guoyin
  • Liao, Pan
  • Xu, Hui
  • Wang, Jing
  • Zhou, Congcong
  • Zhou, Wei
  • Qi, Yaping
  • Xiao, Jianbo
  • Wang, Yuliang
  • Zhang, Lin
subjects:
  • Dan Shen
  • Bioactive compounds
  • Gene expression
  • Metabolic engineering
  • Elicitors
ispartof: Acta Physiologiae Plantarum, 2012, Vol.34(4), pp.1421-1433
description: To develop an optimal bioprocess for the production of tanshinone which is mainly used for the treatment of cardiocerebral vascular disease, the tanshinone biosynthetic pathway regulation must be better understood. In this paper, expression of tanshinone biosynthetic pathway related genes as well as tanshinone accumulation in Salvia miltiorrhiza hairy root cultures were investigated, in response to biotic and abiotic elicitors, respectively. Our results showed tanshinone accumulation in S. miltiorrhiza hairy roots was highly regulated by the coordination of the expression of several genes involved in tanshinone biosynthesis pathway. Our results showed a positive correlation between gene expression and tanshinone accumulation, suggesting that tanshinone accumulation may be the result of the coexpression up-regulation of several genes involved in tanshinone biosynthesis under the treatment of various elicitors. Meantime, SmHMGR , SmDXS2 , SmFPPS , SmGGPPS and SmCPS were identified as the potential key enzymes in the pathway for targeted metabolic engineering to increase accumulation of tanshinone in S. miltiorrhiza hairy roots. This is the first report integrating comprehensively the transcript and metabolite biosynthesis of tanshinone in S. miltiorrhiza hairy roots.
language: eng
source:
identifier: ISSN: 0137-5881 ; E-ISSN: 1861-1664 ; DOI: 10.1007/s11738-012-0940-z
fulltext: fulltext
issn:
  • 1861-1664
  • 18611664
  • 0137-5881
  • 01375881
url: Link


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titleMolecular mechanism of elicitor-induced tanshinone accumulation in Salvia miltiorrhiza hairy root cultures
creatorKai, Guoyin ; Liao, Pan ; Xu, Hui ; Wang, Jing ; Zhou, Congcong ; Zhou, Wei ; Qi, Yaping ; Xiao, Jianbo ; Wang, Yuliang ; Zhang, Lin
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subjectDan Shen ; Bioactive compounds ; Gene expression ; Metabolic engineering ; Elicitors
descriptionTo develop an optimal bioprocess for the production of tanshinone which is mainly used for the treatment of cardiocerebral vascular disease, the tanshinone biosynthetic pathway regulation must be better understood. In this paper, expression of tanshinone biosynthetic pathway related genes as well as tanshinone accumulation in Salvia miltiorrhiza hairy root cultures were investigated, in response to biotic and abiotic elicitors, respectively. Our results showed tanshinone accumulation in S. miltiorrhiza hairy roots was highly regulated by the coordination of the expression of several genes involved in tanshinone biosynthesis pathway. Our results showed a positive correlation between gene expression and tanshinone accumulation, suggesting that tanshinone accumulation may be the result of the coexpression up-regulation of several genes involved in tanshinone biosynthesis under the treatment of various elicitors. Meantime, SmHMGR , SmDXS2 , SmFPPS , SmGGPPS and SmCPS were identified as the potential key enzymes in the pathway for targeted metabolic engineering to increase accumulation of tanshinone in S. miltiorrhiza hairy roots. This is the first report integrating comprehensively the transcript and metabolite biosynthesis of tanshinone in S. miltiorrhiza hairy roots.
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abstractTo develop an optimal bioprocess for the production of tanshinone which is mainly used for the treatment of cardiocerebral vascular disease, the tanshinone biosynthetic pathway regulation must be better understood. In this paper, expression of tanshinone biosynthetic pathway related genes as well as tanshinone accumulation in Salvia miltiorrhiza hairy root cultures were investigated, in response to biotic and abiotic elicitors, respectively. Our results showed tanshinone accumulation in S. miltiorrhiza hairy roots was highly regulated by the coordination of the expression of several genes involved in tanshinone biosynthesis pathway. Our results showed a positive correlation between gene expression and tanshinone accumulation, suggesting that tanshinone accumulation may be the result of the coexpression up-regulation of several genes involved in tanshinone biosynthesis under the treatment of various elicitors. Meantime, SmHMGR , SmDXS2 , SmFPPS , SmGGPPS and SmCPS were identified as the potential key enzymes in the pathway for targeted metabolic engineering to increase accumulation of tanshinone in S. miltiorrhiza hairy roots. This is the first report integrating comprehensively the transcript and metabolite biosynthesis of tanshinone in S. miltiorrhiza hairy roots.
copBerlin/Heidelberg
pubSpringer-Verlag
doi10.1007/s11738-012-0940-z
pages1421-1433
date2012-07