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Alcohol dehydrogenase Ⅰ expression correlates with CDR1, CDR2 and FLU1 expression in Candida albicans from patients with vulvovaginal candidiasis

Funding organization: This study was supported by grants from the National Natural Science Foundation of China(.81171542,30972660)%the Natural Science Foundation of Guangdong Province,China(10151008901000131)%Guangzhou Key Technology RD Program,China(2010J-E011)%the Fundamental Research Funds for th... Full description

Journal Title: 中华医学杂志(英文版) 2013, Vol.126(11), pp.2098-2102
Main Author: GUO Hui
Other Authors: ZHANG Xiao-li , GAO Lai-qiang , LI Shui-xiu , SONG Yan-jun , ZHANG Hong
Format: Electronic Article Electronic Article
Language: chi
Subjects:
ID: ISSN: 0366-6999
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title: Alcohol dehydrogenase Ⅰ expression correlates with CDR1, CDR2 and FLU1 expression in Candida albicans from patients with vulvovaginal candidiasis
format: Article
creator:
  • GUO Hui
  • ZHANG Xiao-li
  • GAO Lai-qiang
  • LI Shui-xiu
  • SONG Yan-jun
  • ZHANG Hong
subjects:
  • Alcohol Dehydrogenase Ⅰ
  • Cdr1
  • Cdr2
  • Flu1
  • Azole Resistance
ispartof: 中华医学杂志(英文版), 2013, Vol.126(11), pp.2098-2102
description: Funding organization: This study was supported by grants from the National Natural Science Foundation of China(.81171542,30972660)%the Natural Science Foundation of Guangdong Province,China(10151008901000131)%Guangzhou Key Technology RD Program,China(2010J-E011)%the Fundamental Research Funds for the Central Universities(21611509)
language: chi
source:
identifier: ISSN: 0366-6999
fulltext: fulltext
issn:
  • 0366-6999
  • 03666999
url: Link


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titleAlcohol dehydrogenase Ⅰ expression correlates with CDR1, CDR2 and FLU1 expression in Candida albicans from patients with vulvovaginal candidiasis
creatorGUO Hui ; ZHANG Xiao-li ; GAO Lai-qiang ; LI Shui-xiu ; SONG Yan-jun ; ZHANG Hong
identifierISSN: 0366-6999
subjectAlcohol Dehydrogenase Ⅰ ; Cdr1 ; Cdr2 ; Flu1 ; Azole Resistance
description
0Funding organization: This study was supported by grants from the National Natural Science Foundation of China(.81171542,30972660)%the Natural Science Foundation of Guangdong Province,China(10151008901000131)%Guangzhou Key Technology RD Program,China(2010J-E011)%the Fundamental Research Funds for the Central Universities(21611509)
1Background The most critical mechanism governing drug resistance in Candida albicans (C.albicans) involves efflux pumps,the functionality of which largely depends on energy metabolism.Alcohol dehydrogenase Ⅰ (ADH1) plays an important role in intracellular energy metabolism.The aim of this study was to explore the relationship between ADH1 and drug resistance in C.albicans.Methods Twenty clinical C.albicans samples isolated from individual patients diagnosed with vulvovaginal candidiasis,and two C.albicans strains obtained from a single parental source (the fluconazole (FLC)-sensitive strain CA-1S and the FLC-resistant strain CA-16R) were included in our study.In accordance with the Clinical and Laboratory Standards Institute (CLSI) M27-A3 guidelines,we used the microdilution method to examine the FLC minimum inhibitory concentrations (MICs) and real-time reverse transcription polymerase chain reaction (RT-PCR) to measure the mRNA expression levels of ADH1 and the azole resistance genes CDR1,CDR2,MDR1,FLU1 and ERG11 in all the isolates.Results A highly significant positive correlation between the mRNA levels of ADH1 and the MICs (rs =0.921,P=0.000),as well as positive correlations between the mRNA level of ADH1 and those of CDR1,CDR2 and FLU1 (rs of 0.704,0.772 and 0.779,respectively,P <0.01),were observed in the 20 clinical C.albicans samples.The relative expression of ADH1 was upregulated 10.63-to 17.61-fold in all of the drug-resistant isolates.No correlations were found between the mRNA levels of ADH1 and those of MDR1 or ERG11 (P >0.05).The mRNA levels of the examined drug resistance genes were higher in the CA-16R strain than in CA-1S,and the mRNA levels of ADH1 in CA-16R were 11.64-fold higher than those in CA-1S (P <0.05).Conclusions These results suggest that high levels of ADH1 transcription are implicated in FLC resistance in C.albicans and that the mRNA expression levels of ADH1 are positively correlated with those of CDR1,CDR2 and FLU1.
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0GUO Hui
1ZHANG Xiao-li
2GAO Lai-qiang
3LI Shui-xiu
4SONG Yan-jun
5ZHANG Hong
titleAlcohol dehydrogenase Ⅰ expression correlates with CDR1, CDR2 and FLU1 expression in Candida albicans from patients with vulvovaginal candidiasis
descriptionBackground The most critical mechanism governing drug resistance in Candida albicans (C.albicans) involves efflux pumps,the functionality of which largely depends on energy metabolism.Alcohol dehydrogenase Ⅰ (ADH1) plays an important role in intracellular energy metabolism.The aim of this study was to explore the relationship between ADH1 and drug resistance in C.albicans.Methods Twenty clinical C.albicans samples isolated from individual patients diagnosed with vulvovaginal candidiasis,and two C.albicans strains obtained from a single parental source (the fluconazole (FLC)-sensitive strain CA-1S and the FLC-resistant strain CA-16R) were included in our study.In accordance with the Clinical and Laboratory Standards Institute (CLSI) M27-A3 guidelines,we used the microdilution method to examine the FLC minimum inhibitory concentrations (MICs) and real-time reverse transcription polymerase chain reaction (RT-PCR) to measure the mRNA expression levels of ADH1 and the azole resistance genes CDR1,CDR2,MDR1,FLU1 and ERG11 in all the isolates.Results A highly significant positive correlation between the mRNA levels of ADH1 and the MICs (rs =0.921,P=0.000),as well as positive correlations between the mRNA level of ADH1 and those of CDR1,CDR2 and FLU1 (rs of 0.704,0.772 and 0.779,respectively,P <0.01),were observed in the 20 clinical C.albicans samples.The relative expression of ADH1 was upregulated 10.63-to 17.61-fold in all of the drug-resistant isolates.No correlations were found between the mRNA levels of ADH1 and those of MDR1 or ERG11 (P >0.05).The mRNA levels of the examined drug resistance genes were higher in the CA-16R strain than in CA-1S,and the mRNA levels of ADH1 in CA-16R were 11.64-fold higher than those in CA-1S (P <0.05).Conclusions These results suggest that high levels of ADH1 transcription are implicated in FLC resistance in C.albicans and that the mRNA expression levels of ADH1 are positively correlated with those of CDR1,CDR2 and FLU1.
subject
0Alcohol Dehydrogenase Ⅰ
1Cdr1
2Cdr2
3Flu1
4Azole Resistance
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2This study was supported by grants from the National Natural Science Foundation of China(.81171542,30972660)%the Natural Science Foundation of Guangdong Province,China(10151008901000131)%Guangzhou Key Technology RD Program,China(2010J-E011)%the Fundamental Research Funds for the Central Universities(21611509)
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titleAlcohol dehydrogenase Ⅰ expression correlates with CDR1, CDR2 and FLU1 expression in Candida albicans from patients with vulvovaginal candidiasis
authorGUO Hui ; ZHANG Xiao-li ; GAO Lai-qiang ; LI Shui-xiu ; SONG Yan-jun ; ZHANG Hong
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0GUO Hui
1ZHANG Xiao-li
2GAO Lai-qiang
3LI Shui-xiu
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1ZHANG Xiao-li
2GAO Lai-qiang
3LI Shui-xiu
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atitleAlcohol dehydrogenase Ⅰ expression correlates with CDR1, CDR2 and FLU1 expression in Candida albicans from patients with vulvovaginal candidiasis
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abstractBackground The most critical mechanism governing drug resistance in Candida albicans (C.albicans) involves efflux pumps,the functionality of which largely depends on energy metabolism.Alcohol dehydrogenase Ⅰ (ADH1) plays an important role in intracellular energy metabolism.The aim of this study was to explore the relationship between ADH1 and drug resistance in C.albicans.Methods Twenty clinical C.albicans samples isolated from individual patients diagnosed with vulvovaginal candidiasis,and two C.albicans strains obtained from a single parental source (the fluconazole (FLC)-sensitive strain CA-1S and the FLC-resistant strain CA-16R) were included in our study.In accordance with the Clinical and Laboratory Standards Institute (CLSI) M27-A3 guidelines,we used the microdilution method to examine the FLC minimum inhibitory concentrations (MICs) and real-time reverse transcription polymerase chain reaction (RT-PCR) to measure the mRNA expression levels of ADH1 and the azole resistance genes CDR1,CDR2,MDR1,FLU1 and ERG11 in all the isolates.Results A highly significant positive correlation between the mRNA levels of ADH1 and the MICs (rs =0.921,P=0.000),as well as positive correlations between the mRNA level of ADH1 and those of CDR1,CDR2 and FLU1 (rs of 0.704,0.772 and 0.779,respectively,P <0.01),were observed in the 20 clinical C.albicans samples.The relative expression of ADH1 was upregulated 10.63-to 17.61-fold in all of the drug-resistant isolates.No correlations were found between the mRNA levels of ADH1 and those of MDR1 or ERG11 (P >0.05).The mRNA levels of the examined drug resistance genes were higher in the CA-16R strain than in CA-1S,and the mRNA levels of ADH1 in CA-16R were 11.64-fold higher than those in CA-1S (P <0.05).Conclusions These results suggest that high levels of ADH1 transcription are implicated in FLC resistance in C.albicans and that the mRNA expression levels of ADH1 are positively correlated with those of CDR1,CDR2 and FLU1.
doi10.3760/cma.j.issn.0366-6999.20123605