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Pyrolysed 3D‐Carbon Scaffolds Induce Spontaneous Differentiation of Human Neural Stem Cells and Facilitate Real‐Time Dopamine Detection

Structurally patterned pyrolysed three‐dimensional carbon scaffolds (p3D‐carbon) are fabricated and applied for differentiation of human neural stem cells (hNSCs) developed for cell replacement therapy and sensing of released dopamine. In the absence of differentiation factors (DF) the pyrolysed car... Full description

Journal Title: Advanced Functional Materials November 2014, Vol.24(44), pp.7042-7052
Main Author: Amato, Letizia
Other Authors: Heiskanen, Arto , Caviglia, Claudia , Shah, Fozia , Zór, Kinga , Skolimowski, Maciej , Madou, Marc , Gammelgaard, Lauge , Hansen, Rasmus , Seiz, Emma G. , Ramos, Milagros , Moreno, Tania Ramos , Martínez‐Serrano, Alberto , Keller, Stephan S. , Emnéus, Jenny
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ID: ISSN: 1616-301X ; E-ISSN: 1616-3028 ; DOI: 10.1002/adfm.201400812
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title: Pyrolysed 3D‐Carbon Scaffolds Induce Spontaneous Differentiation of Human Neural Stem Cells and Facilitate Real‐Time Dopamine Detection
format: Article
creator:
  • Amato, Letizia
  • Heiskanen, Arto
  • Caviglia, Claudia
  • Shah, Fozia
  • Zór, Kinga
  • Skolimowski, Maciej
  • Madou, Marc
  • Gammelgaard, Lauge
  • Hansen, Rasmus
  • Seiz, Emma G.
  • Ramos, Milagros
  • Moreno, Tania Ramos
  • Martínez‐Serrano, Alberto
  • Keller, Stephan S.
  • Emnéus, Jenny
subjects:
  • Pyrolysed Carbon Scaffolds
  • 3d Cultures
  • Human Neural Stem Cells
  • Dopaminergic Neurons
  • Electrochemical Dopamine Detections
ispartof: Advanced Functional Materials, November 2014, Vol.24(44), pp.7042-7052
description: Structurally patterned pyrolysed three‐dimensional carbon scaffolds (p3D‐carbon) are fabricated and applied for differentiation of human neural stem cells (hNSCs) developed for cell replacement therapy and sensing of released dopamine. In the absence of differentiation factors (DF) the pyrolysed carbon material induces spontaneous hNSC differentiation into mature dopamine‐producing neurons and the 3D‐topography promotes neurite elongation. In the presence and absence of DF, ≈73–82% of the hNSCs obtain dopaminergic properties on pyrolysed carbon, a to‐date unseen efficiency in both two‐dimensional (2D) and 3D environment. Due to conductive properties and 3D environment, the p3D‐carbon serves as a neurotransmitter trap, enabling electrochemical detection of a significantly larger dopamine fraction released by the hNSC derived neurons than on conventional 2D electrodes. This is the first study of its kind, presenting new conductive 3D scaffolds that provide highly efficient hNSC differentiation to dopaminergic phenotype combined with real‐time in situ confirmation of the fate of the hNSC‐derived neurons. dimensionally reaching the limit of UV‐lithography are for the first time presented and applied for differentiation of human neural stem cells (hNSCs), demonstrating the uniqueness of pyrolysed carbon as a material that induces spontaneous differentiation in 80% of hNSCs into dopaminergic neurons. The scaffold simultaneously serves as a mechanical and biocompatible support and 3D electrochemical sensor for dopamine detection.
language:
source:
identifier: ISSN: 1616-301X ; E-ISSN: 1616-3028 ; DOI: 10.1002/adfm.201400812
fulltext: fulltext
issn:
  • 1616-301X
  • 1616301X
  • 1616-3028
  • 16163028
url: Link


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titlePyrolysed 3D‐Carbon Scaffolds Induce Spontaneous Differentiation of Human Neural Stem Cells and Facilitate Real‐Time Dopamine Detection
creatorAmato, Letizia ; Heiskanen, Arto ; Caviglia, Claudia ; Shah, Fozia ; Zór, Kinga ; Skolimowski, Maciej ; Madou, Marc ; Gammelgaard, Lauge ; Hansen, Rasmus ; Seiz, Emma G. ; Ramos, Milagros ; Moreno, Tania Ramos ; Martínez‐Serrano, Alberto ; Keller, Stephan S. ; Emnéus, Jenny
ispartofAdvanced Functional Materials, November 2014, Vol.24(44), pp.7042-7052
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subjectPyrolysed Carbon Scaffolds ; 3d Cultures ; Human Neural Stem Cells ; Dopaminergic Neurons ; Electrochemical Dopamine Detections
descriptionStructurally patterned pyrolysed three‐dimensional carbon scaffolds (p3D‐carbon) are fabricated and applied for differentiation of human neural stem cells (hNSCs) developed for cell replacement therapy and sensing of released dopamine. In the absence of differentiation factors (DF) the pyrolysed carbon material induces spontaneous hNSC differentiation into mature dopamine‐producing neurons and the 3D‐topography promotes neurite elongation. In the presence and absence of DF, ≈73–82% of the hNSCs obtain dopaminergic properties on pyrolysed carbon, a to‐date unseen efficiency in both two‐dimensional (2D) and 3D environment. Due to conductive properties and 3D environment, the p3D‐carbon serves as a neurotransmitter trap, enabling electrochemical detection of a significantly larger dopamine fraction released by the hNSC derived neurons than on conventional 2D electrodes. This is the first study of its kind, presenting new conductive 3D scaffolds that provide highly efficient hNSC differentiation to dopaminergic phenotype combined with real‐time in situ confirmation of the fate of the hNSC‐derived neurons. dimensionally reaching the limit of UV‐lithography are for the first time presented and applied for differentiation of human neural stem cells (hNSCs), demonstrating the uniqueness of pyrolysed carbon as a material that induces spontaneous differentiation in 80% of hNSCs into dopaminergic neurons. The scaffold simultaneously serves as a mechanical and biocompatible support and 3D electrochemical sensor for dopamine detection.
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titlePyrolysed 3D‐Carbon Scaffolds Induce Spontaneous Differentiation of Human Neural Stem Cells and Facilitate Real‐Time Dopamine Detection
descriptionStructurally patterned pyrolysed three‐dimensional carbon scaffolds (p3D‐carbon) are fabricated and applied for differentiation of human neural stem cells (hNSCs) developed for cell replacement therapy and sensing of released dopamine. In the absence of differentiation factors (DF) the pyrolysed carbon material induces spontaneous hNSC differentiation into mature dopamine‐producing neurons and the 3D‐topography promotes neurite elongation. In the presence and absence of DF, ≈73–82% of the hNSCs obtain dopaminergic properties on pyrolysed carbon, a to‐date unseen efficiency in both two‐dimensional (2D) and 3D environment. Due to conductive properties and 3D environment, the p3D‐carbon serves as a neurotransmitter trap, enabling electrochemical detection of a significantly larger dopamine fraction released by the hNSC derived neurons than on conventional 2D electrodes. This is the first study of its kind, presenting new conductive 3D scaffolds that provide highly efficient hNSC differentiation to dopaminergic phenotype combined with real‐time in situ confirmation of the fate of the hNSC‐derived neurons. dimensionally reaching the limit of UV‐lithography are for the first time presented and applied for differentiation of human neural stem cells (hNSCs), demonstrating the uniqueness of pyrolysed carbon as a material that induces spontaneous differentiation in 80% of hNSCs into dopaminergic neurons. The scaffold simultaneously serves as a mechanical and biocompatible support and 3D electrochemical sensor for dopamine detection.
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titlePyrolysed 3D‐Carbon Scaffolds Induce Spontaneous Differentiation of Human Neural Stem Cells and Facilitate Real‐Time Dopamine Detection
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abstractStructurally patterned pyrolysed three‐dimensional carbon scaffolds (p3D‐carbon) are fabricated and applied for differentiation of human neural stem cells (hNSCs) developed for cell replacement therapy and sensing of released dopamine. In the absence of differentiation factors (DF) the pyrolysed carbon material induces spontaneous hNSC differentiation into mature dopamine‐producing neurons and the 3D‐topography promotes neurite elongation. In the presence and absence of DF, ≈73–82% of the hNSCs obtain dopaminergic properties on pyrolysed carbon, a to‐date unseen efficiency in both two‐dimensional (2D) and 3D environment. Due to conductive properties and 3D environment, the p3D‐carbon serves as a neurotransmitter trap, enabling electrochemical detection of a significantly larger dopamine fraction released by the hNSC derived neurons than on conventional 2D electrodes. This is the first study of its kind, presenting new conductive 3D scaffolds that provide highly efficient hNSC differentiation to dopaminergic phenotype combined with real‐time in situ confirmation of the fate of the hNSC‐derived neurons. dimensionally reaching the limit of UV‐lithography are for the first time presented and applied for differentiation of human neural stem cells (hNSCs), demonstrating the uniqueness of pyrolysed carbon as a material that induces spontaneous differentiation in 80% of hNSCs into dopaminergic neurons. The scaffold simultaneously serves as a mechanical and biocompatible support and 3D electrochemical sensor for dopamine detection.
doi10.1002/adfm.201400812
pages7042-7052
date2014-11