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A fluorescent aptasensor for sensitive analysis oxytetracycline based on silver nanoclusters

A fluorescent aptasensor for detection of oxytetracycline (OTC) was presented based on fluorescence quenching of DNA aptamer‐templated silver nanoclusters (AgNCs). The specific DNA scaffolds with two different nucleotides fragments were used: one was enriched with a cytosine sequence fragment (C12)... Full description

Journal Title: Luminescence November 2016, Vol.31(7), pp.1339-1343
Main Author: Hosseini, Morteza
Other Authors: Mehrabi, Fatemeh , Ganjali, Mohammad Reza , Norouzi, Parviz
Format: Electronic Article Electronic Article
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ID: ISSN: 1522-7235 ; E-ISSN: 1522-7243 ; DOI: 10.1002/bio.3112
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recordid: wj10.1002/bio.3112
title: A fluorescent aptasensor for sensitive analysis oxytetracycline based on silver nanoclusters
format: Article
creator:
  • Hosseini, Morteza
  • Mehrabi, Fatemeh
  • Ganjali, Mohammad Reza
  • Norouzi, Parviz
subjects:
  • Oxytetracycline
  • Silver Nanoclusters
  • Fluorescence
  • Quenching
  • Aptamer
  • Nanobiosensor
ispartof: Luminescence, November 2016, Vol.31(7), pp.1339-1343
description: A fluorescent aptasensor for detection of oxytetracycline (OTC) was presented based on fluorescence quenching of DNA aptamer‐templated silver nanoclusters (AgNCs). The specific DNA scaffolds with two different nucleotides fragments were used: one was enriched with a cytosine sequence fragment (C12) that could produce DNA–AgNCs via a chemical reduction method, and another was the OTC aptamer fragment that could selectively bind to the OTC antibiotic. Thus, the as‐prepared AgNCs could exhibit quenched fluorescence after binding to the target OTC. The fluorescence ratio of the DNA–AgNCs was quenched in a linearly proportional manner to the concentration of the target in the range of 0.5 nM to 100 nM with a detection limit of 0.1 nM. This proposed nanobiosensor was demonstrated to be sensitive, selective, and simple, introducing a viable alternative for rapid determination of toxin OTC in honey and water samples. Copyright © 2016 John Wiley & Sons, Ltd.
language:
source:
identifier: ISSN: 1522-7235 ; E-ISSN: 1522-7243 ; DOI: 10.1002/bio.3112
fulltext: fulltext
issn:
  • 1522-7235
  • 15227235
  • 1522-7243
  • 15227243
url: Link


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titleA fluorescent aptasensor for sensitive analysis oxytetracycline based on silver nanoclusters
creatorHosseini, Morteza ; Mehrabi, Fatemeh ; Ganjali, Mohammad Reza ; Norouzi, Parviz
ispartofLuminescence, November 2016, Vol.31(7), pp.1339-1343
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subjectOxytetracycline ; Silver Nanoclusters ; Fluorescence ; Quenching ; Aptamer ; Nanobiosensor
descriptionA fluorescent aptasensor for detection of oxytetracycline (OTC) was presented based on fluorescence quenching of DNA aptamer‐templated silver nanoclusters (AgNCs). The specific DNA scaffolds with two different nucleotides fragments were used: one was enriched with a cytosine sequence fragment (C12) that could produce DNA–AgNCs via a chemical reduction method, and another was the OTC aptamer fragment that could selectively bind to the OTC antibiotic. Thus, the as‐prepared AgNCs could exhibit quenched fluorescence after binding to the target OTC. The fluorescence ratio of the DNA–AgNCs was quenched in a linearly proportional manner to the concentration of the target in the range of 0.5 nM to 100 nM with a detection limit of 0.1 nM. This proposed nanobiosensor was demonstrated to be sensitive, selective, and simple, introducing a viable alternative for rapid determination of toxin OTC in honey and water samples. Copyright © 2016 John Wiley & Sons, Ltd.
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titleA fluorescent aptasensor for sensitive analysis oxytetracycline based on silver nanoclusters
descriptionA fluorescent aptasensor for detection of oxytetracycline (OTC) was presented based on fluorescence quenching of DNA aptamer‐templated silver nanoclusters (AgNCs). The specific DNA scaffolds with two different nucleotides fragments were used: one was enriched with a cytosine sequence fragment (C12) that could produce DNA–AgNCs via a chemical reduction method, and another was the OTC aptamer fragment that could selectively bind to the OTC antibiotic. Thus, the as‐prepared AgNCs could exhibit quenched fluorescence after binding to the target OTC. The fluorescence ratio of the DNA–AgNCs was quenched in a linearly proportional manner to the concentration of the target in the range of 0.5 nM to 100 nM with a detection limit of 0.1 nM. This proposed nanobiosensor was demonstrated to be sensitive, selective, and simple, introducing a viable alternative for rapid determination of toxin OTC in honey and water samples. Copyright © 2016 John Wiley & Sons, Ltd.
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abstractA fluorescent aptasensor for detection of oxytetracycline (OTC) was presented based on fluorescence quenching of DNA aptamer‐templated silver nanoclusters (AgNCs). The specific DNA scaffolds with two different nucleotides fragments were used: one was enriched with a cytosine sequence fragment (C12) that could produce DNA–AgNCs via a chemical reduction method, and another was the OTC aptamer fragment that could selectively bind to the OTC antibiotic. Thus, the as‐prepared AgNCs could exhibit quenched fluorescence after binding to the target OTC. The fluorescence ratio of the DNA–AgNCs was quenched in a linearly proportional manner to the concentration of the target in the range of 0.5 nM to 100 nM with a detection limit of 0.1 nM. This proposed nanobiosensor was demonstrated to be sensitive, selective, and simple, introducing a viable alternative for rapid determination of toxin OTC in honey and water samples. Copyright © 2016 John Wiley & Sons, Ltd.
doi10.1002/bio.3112
pages1339-1343
date2016-11