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Existence of Diverse Modifications in Small‐RNA Species Composed of 16–28 Nucleotides

RNA contains diverse modifications that exert an important influence in a variety of cellular processes. So far, more than 150 modifications have been identified in various RNA species, mainly in ribosomal RNA (rRNA), transfer RNA (tRNA), and messenger RNA (mRNA). In contrast to rRNA, tRNA, and mRNA... Full description

Journal Title: Chemistry – A European Journal 11 July 2018, Vol.24(39), pp.9949-9956
Main Author: Lan, Meng‐Dan
Other Authors: Xiong, Jun , You, Xue‐Jiao , Weng, Xiao‐Cheng , Zhou, Xiang , Yuan, Bi‐Feng , Feng, Yu‐Qi
Format: Electronic Article Electronic Article
Language: English
Subjects:
Rna
ID: ISSN: 0947-6539 ; E-ISSN: 1521-3765 ; DOI: 10.1002/chem.201801640
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recordid: wj10.1002/chem.201801640
title: Existence of Diverse Modifications in Small‐RNA Species Composed of 16–28 Nucleotides
format: Article
creator:
  • Lan, Meng‐Dan
  • Xiong, Jun
  • You, Xue‐Jiao
  • Weng, Xiao‐Cheng
  • Zhou, Xiang
  • Yuan, Bi‐Feng
  • Feng, Yu‐Qi
subjects:
  • Enzymes
  • Mass Spectrometry
  • Modification
  • Neutral Enzymatic Digestion
  • Obesity-Associated Proteins
  • Rna
ispartof: Chemistry – A European Journal, 11 July 2018, Vol.24(39), pp.9949-9956
description: RNA contains diverse modifications that exert an important influence in a variety of cellular processes. So far, more than 150 modifications have been identified in various RNA species, mainly in ribosomal RNA (rRNA), transfer RNA (tRNA), and messenger RNA (mRNA). In contrast to rRNA, tRNA, and mRNA, the known modifications in small RNA species have been primarily limited to 2′‐‐ribose methylation in plants and inosine in mammals. The methylation of small RNAs in mammals is still unclear. Current methods widely used in the characterization of small RNAs are mainly based on the strategy of nucleic acid hybridization and sequencing, which cannot characterize modifications in small RNAs. Herein, we have systematically investigated modifications in small RNAs composed of 16–28 nucleotides (nt) by establishing an effective isolation and neutral enzymatic digestion of small RNAs in combination with liquid chromatography/electrospray ionization tandem mass spectrometry (LC‐ESI‐MS/MS). This method allowed us to simultaneously detect 57 different types of nucleoside modification. By using this approach, we revealed 24 modifications in small RNAs comprising 16–28 nt from human cells. In addition, we found that the obesity‐associated protein (FTO) may demethylate ‐methyladenosine (mA) and ,2′‐‐dimethyladenosine (mAm) in small RNAs of 16–28 nt. Our study demonstrates the existence of diverse modifications in small RNAs composed of 16–28 nt, which may promote in‐depth understanding of the regulatory roles of noncoding RNAs. : Modifications in small RNAs composed of 16–28 nucleotides (nt) by establishing an effective isolation and neutral enzymatic digestion of small RNAs in combination with liquid chromatography/electrospray ionization tandem mass spectrometry (LC‐ESI‐MS/MS; see figure) were systematically investigated . Our study demonstrates the existence of diverse modifications in small RNAs comprising 16–28 nt, which may promote in‐depth understanding of the regulatory roles of noncoding RNAs.
language: eng
source:
identifier: ISSN: 0947-6539 ; E-ISSN: 1521-3765 ; DOI: 10.1002/chem.201801640
fulltext: fulltext
issn:
  • 0947-6539
  • 09476539
  • 1521-3765
  • 15213765
url: Link


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titleExistence of Diverse Modifications in Small‐RNA Species Composed of 16–28 Nucleotides
creatorLan, Meng‐Dan ; Xiong, Jun ; You, Xue‐Jiao ; Weng, Xiao‐Cheng ; Zhou, Xiang ; Yuan, Bi‐Feng ; Feng, Yu‐Qi
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subjectEnzymes ; Mass Spectrometry ; Modification ; Neutral Enzymatic Digestion ; Obesity-Associated Proteins ; Rna
descriptionRNA contains diverse modifications that exert an important influence in a variety of cellular processes. So far, more than 150 modifications have been identified in various RNA species, mainly in ribosomal RNA (rRNA), transfer RNA (tRNA), and messenger RNA (mRNA). In contrast to rRNA, tRNA, and mRNA, the known modifications in small RNA species have been primarily limited to 2′‐‐ribose methylation in plants and inosine in mammals. The methylation of small RNAs in mammals is still unclear. Current methods widely used in the characterization of small RNAs are mainly based on the strategy of nucleic acid hybridization and sequencing, which cannot characterize modifications in small RNAs. Herein, we have systematically investigated modifications in small RNAs composed of 16–28 nucleotides (nt) by establishing an effective isolation and neutral enzymatic digestion of small RNAs in combination with liquid chromatography/electrospray ionization tandem mass spectrometry (LC‐ESI‐MS/MS). This method allowed us to simultaneously detect 57 different types of nucleoside modification. By using this approach, we revealed 24 modifications in small RNAs comprising 16–28 nt from human cells. In addition, we found that the obesity‐associated protein (FTO) may demethylate ‐methyladenosine (mA) and ,2′‐‐dimethyladenosine (mAm) in small RNAs of 16–28 nt. Our study demonstrates the existence of diverse modifications in small RNAs composed of 16–28 nt, which may promote in‐depth understanding of the regulatory roles of noncoding RNAs. : Modifications in small RNAs composed of 16–28 nucleotides (nt) by establishing an effective isolation and neutral enzymatic digestion of small RNAs in combination with liquid chromatography/electrospray ionization tandem mass spectrometry (LC‐ESI‐MS/MS; see figure) were systematically investigated . Our study demonstrates the existence of diverse modifications in small RNAs comprising 16–28 nt, which may promote in‐depth understanding of the regulatory roles of noncoding RNAs.
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titleExistence of Diverse Modifications in Small‐RNA Species Composed of 16–28 Nucleotides
descriptionRNA contains diverse modifications that exert an important influence in a variety of cellular processes. So far, more than 150 modifications have been identified in various RNA species, mainly in ribosomal RNA (rRNA), transfer RNA (tRNA), and messenger RNA (mRNA). In contrast to rRNA, tRNA, and mRNA, the known modifications in small RNA species have been primarily limited to 2′‐‐ribose methylation in plants and inosine in mammals. The methylation of small RNAs in mammals is still unclear. Current methods widely used in the characterization of small RNAs are mainly based on the strategy of nucleic acid hybridization and sequencing, which cannot characterize modifications in small RNAs. Herein, we have systematically investigated modifications in small RNAs composed of 16–28 nucleotides (nt) by establishing an effective isolation and neutral enzymatic digestion of small RNAs in combination with liquid chromatography/electrospray ionization tandem mass spectrometry (LC‐ESI‐MS/MS). This method allowed us to simultaneously detect 57 different types of nucleoside modification. By using this approach, we revealed 24 modifications in small RNAs comprising 16–28 nt from human cells. In addition, we found that the obesity‐associated protein (FTO) may demethylate ‐methyladenosine (mA) and ,2′‐‐dimethyladenosine (mAm) in small RNAs of 16–28 nt. Our study demonstrates the existence of diverse modifications in small RNAs composed of 16–28 nt, which may promote in‐depth understanding of the regulatory roles of noncoding RNAs. : Modifications in small RNAs composed of 16–28 nucleotides (nt) by establishing an effective isolation and neutral enzymatic digestion of small RNAs in combination with liquid chromatography/electrospray ionization tandem mass spectrometry (LC‐ESI‐MS/MS; see figure) were systematically investigated . Our study demonstrates the existence of diverse modifications in small RNAs comprising 16–28 nt, which may promote in‐depth understanding of the regulatory roles of noncoding RNAs.
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titleExistence of Diverse Modifications in Small‐RNA Species Composed of 16–28 Nucleotides
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abstractRNA contains diverse modifications that exert an important influence in a variety of cellular processes. So far, more than 150 modifications have been identified in various RNA species, mainly in ribosomal RNA (rRNA), transfer RNA (tRNA), and messenger RNA (mRNA). In contrast to rRNA, tRNA, and mRNA, the known modifications in small RNA species have been primarily limited to 2′‐‐ribose methylation in plants and inosine in mammals. The methylation of small RNAs in mammals is still unclear. Current methods widely used in the characterization of small RNAs are mainly based on the strategy of nucleic acid hybridization and sequencing, which cannot characterize modifications in small RNAs. Herein, we have systematically investigated modifications in small RNAs composed of 16–28 nucleotides (nt) by establishing an effective isolation and neutral enzymatic digestion of small RNAs in combination with liquid chromatography/electrospray ionization tandem mass spectrometry (LC‐ESI‐MS/MS). This method allowed us to simultaneously detect 57 different types of nucleoside modification. By using this approach, we revealed 24 modifications in small RNAs comprising 16–28 nt from human cells. In addition, we found that the obesity‐associated protein (FTO) may demethylate ‐methyladenosine (mA) and ,2′‐‐dimethyladenosine (mAm) in small RNAs of 16–28 nt. Our study demonstrates the existence of diverse modifications in small RNAs composed of 16–28 nt, which may promote in‐depth understanding of the regulatory roles of noncoding RNAs. : Modifications in small RNAs composed of 16–28 nucleotides (nt) by establishing an effective isolation and neutral enzymatic digestion of small RNAs in combination with liquid chromatography/electrospray ionization tandem mass spectrometry (LC‐ESI‐MS/MS; see figure) were systematically investigated . Our study demonstrates the existence of diverse modifications in small RNAs comprising 16–28 nt, which may promote in‐depth understanding of the regulatory roles of noncoding RNAs.
doi10.1002/chem.201801640
orcididhttp://orcid.org/0000-0001-5223-4659
pages9949-9956
date2018-07-11