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Antitumor effect of CXCR4 antagonist AMD3100 on the tumorigenic cell line of BHP10‐3 papillary thyroid cancer cells

Byline: Young Ho Jung, Doh Young Lee, Wonjae Cha, Bo Hae Kim, Myung-Whun Sung, Kwang Hyun Kim, Soon-Hyun Ahn Abstract Background A tumorigenic cell line (BHP10-3M) derived from nontumorigenic papillary thyroid carcinoma (PTC) cells (BHP10-3) having rearranged during transfection (RET)/PTC1 gene rear... Full description

Journal Title: Head & Neck October 2016, Vol.38(10), pp.1479-1486
Main Author: Jung, Young Ho
Other Authors: Lee, Doh Young , Cha, Wonjae , Kim, Bo Hae , Sung, Myung‐Whun , Kim, Kwang Hyun , Ahn, Soon‐Hyun
Format: Electronic Article Electronic Article
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ID: ISSN: 1043-3074 ; E-ISSN: 1097-0347 ; DOI: 10.1002/hed.24461
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recordid: wj10.1002/hed.24461
title: Antitumor effect of CXCR4 antagonist AMD3100 on the tumorigenic cell line of BHP10‐3 papillary thyroid cancer cells
format: Article
creator:
  • Jung, Young Ho
  • Lee, Doh Young
  • Cha, Wonjae
  • Kim, Bo Hae
  • Sung, Myung‐Whun
  • Kim, Kwang Hyun
  • Ahn, Soon‐Hyun
subjects:
  • Cxcr4
  • Cd24
  • Amd3100
  • Papillary Thyroid Cancer
  • Tumorigenic Cell
ispartof: Head & Neck, October 2016, Vol.38(10), pp.1479-1486
description: Byline: Young Ho Jung, Doh Young Lee, Wonjae Cha, Bo Hae Kim, Myung-Whun Sung, Kwang Hyun Kim, Soon-Hyun Ahn Abstract Background A tumorigenic cell line (BHP10-3M) derived from nontumorigenic papillary thyroid carcinoma (PTC) cells (BHP10-3) having rearranged during transfection (RET)/PTC1 gene rearrangement might have a higher expression of CXCR4, either quantitatively or functionally. The authors also postulated that CXCR4-mediated invasion or tumorigenesis could be blocked by CXCR4 antagonists, including AMD3100. Methods The expression of CXCR4 in BHP10-3 and BHP10-3M cells was assessed using immunoblot analysis, flow cytometry, and quantitative reverse-transcriptase polymerase chain reaction (RT-PCR). The effect of AMD3100 on BHP10-3 and BHP10-3M cell lines was evaluated using cell proliferation assay, invasion assay, and tumor growth experiment in nude mice. Results Immunoblotting, flow cytometry, and quantitative RT-PCR proved that BHP10-3M cells expressed a higher level of CXCR4 than BHP10-3 cells. Although blocking CXCR4 with AMD3100 did not suppress cell proliferation in both cell lines from 1 ng/mL to 100 ng/mL concentration, AMD3100 suppressed invasion of BHP10-3M cells in vitro in a dose-dependent manner. At higher concentrations from 10.sub.3 ng/mL to 10.sub.5 ng/mL, the proliferation of BHP10-3M cells was inhibited more strongly by AMD3100 than that of BHP10-3 cells. Intraperitoneal injection of AMD3100 inhibited tumor formation by BHP10-3M cells in the thyroid of nude mice. Conclusion A tumorigenic cell line (BHP10-3M) of PTC showed higher expression of CXCR4 quantitatively and functionally than a nontumorigenic cell line (BHP10-3). The CXCR4 antagonist (AMD3100) showed a significant antitumor effect on the tumorigenic cell line of PTC BHP10-3 cells both in vitro and in vivo. CXCR4 antagonist can be expected to have an adjuvant role in the management of PTC. [c] 2016 Wiley Periodicals, Inc. Head Neck, 2016 [c] 2016 Wiley Periodicals, Inc. Head Neck 38: First-1486, 2016
language:
source:
identifier: ISSN: 1043-3074 ; E-ISSN: 1097-0347 ; DOI: 10.1002/hed.24461
fulltext: fulltext
issn:
  • 1043-3074
  • 10433074
  • 1097-0347
  • 10970347
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titleAntitumor effect of CXCR4 antagonist AMD3100 on the tumorigenic cell line of BHP10‐3 papillary thyroid cancer cells
creatorJung, Young Ho ; Lee, Doh Young ; Cha, Wonjae ; Kim, Bo Hae ; Sung, Myung‐Whun ; Kim, Kwang Hyun ; Ahn, Soon‐Hyun
ispartofHead & Neck, October 2016, Vol.38(10), pp.1479-1486
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subjectCxcr4 ; Cd24 ; Amd3100 ; Papillary Thyroid Cancer ; Tumorigenic Cell
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descriptionByline: Young Ho Jung, Doh Young Lee, Wonjae Cha, Bo Hae Kim, Myung-Whun Sung, Kwang Hyun Kim, Soon-Hyun Ahn Abstract Background A tumorigenic cell line (BHP10-3M) derived from nontumorigenic papillary thyroid carcinoma (PTC) cells (BHP10-3) having rearranged during transfection (RET)/PTC1 gene rearrangement might have a higher expression of CXCR4, either quantitatively or functionally. The authors also postulated that CXCR4-mediated invasion or tumorigenesis could be blocked by CXCR4 antagonists, including AMD3100. Methods The expression of CXCR4 in BHP10-3 and BHP10-3M cells was assessed using immunoblot analysis, flow cytometry, and quantitative reverse-transcriptase polymerase chain reaction (RT-PCR). The effect of AMD3100 on BHP10-3 and BHP10-3M cell lines was evaluated using cell proliferation assay, invasion assay, and tumor growth experiment in nude mice. Results Immunoblotting, flow cytometry, and quantitative RT-PCR proved that BHP10-3M cells expressed a higher level of CXCR4 than BHP10-3 cells. Although blocking CXCR4 with AMD3100 did not suppress cell proliferation in both cell lines from 1 ng/mL to 100 ng/mL concentration, AMD3100 suppressed invasion of BHP10-3M cells in vitro in a dose-dependent manner. At higher concentrations from 10.sub.3 ng/mL to 10.sub.5 ng/mL, the proliferation of BHP10-3M cells was inhibited more strongly by AMD3100 than that of BHP10-3 cells. Intraperitoneal injection of AMD3100 inhibited tumor formation by BHP10-3M cells in the thyroid of nude mice. Conclusion A tumorigenic cell line (BHP10-3M) of PTC showed higher expression of CXCR4 quantitatively and functionally than a nontumorigenic cell line (BHP10-3). The CXCR4 antagonist (AMD3100) showed a significant antitumor effect on the tumorigenic cell line of PTC BHP10-3 cells both in vitro and in vivo. CXCR4 antagonist can be expected to have an adjuvant role in the management of PTC. [c] 2016 Wiley Periodicals, Inc. Head Neck, 2016 [c] 2016 Wiley Periodicals, Inc. Head Neck 38: First-1486, 2016
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