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Sustained delivery of bioactive TGF‐β1 from self‐assembling peptide hydrogels induces chondrogenesis of encapsulated bone marrow stromal cells

Tissue engineering strategies for cartilage defect repair require technology for local targeted delivery of chondrogenic and anti‐inflammatory factors. The objective of this study was to determine the release kinetics of transforming growth factor β1 (TGF‐β1) from self‐assembling peptide hydrogels,... Full description

Journal Title: Journal of Biomedical Materials Research Part A May 2014, Vol.102(5), pp.1275-1285
Main Author: Kopesky, Paul W.
Other Authors: Byun, Sangwon , Vanderploeg, Eric J. , Kisiday, John D. , Frisbie, David D. , Grodzinsky, Alan J.
Format: Electronic Article Electronic Article
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ID: ISSN: 1549-3296 ; E-ISSN: 1552-4965 ; DOI: 10.1002/jbm.a.34789
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recordid: wj10.1002/jbm.a.34789
title: Sustained delivery of bioactive TGF‐β1 from self‐assembling peptide hydrogels induces chondrogenesis of encapsulated bone marrow stromal cells
format: Article
creator:
  • Kopesky, Paul W.
  • Byun, Sangwon
  • Vanderploeg, Eric J.
  • Kisiday, John D.
  • Frisbie, David D.
  • Grodzinsky, Alan J.
subjects:
  • Tissue Engineering
  • Sustained Delivery
  • Bone Marrow Stromal Cell
  • Regenerative Medicine
  • Cartilage Repair
ispartof: Journal of Biomedical Materials Research Part A, May 2014, Vol.102(5), pp.1275-1285
description: Tissue engineering strategies for cartilage defect repair require technology for local targeted delivery of chondrogenic and anti‐inflammatory factors. The objective of this study was to determine the release kinetics of transforming growth factor β1 (TGF‐β1) from self‐assembling peptide hydrogels, a candidate scaffold for cell transplant therapies, and stimulate chondrogenesis of encapsulated young equine bone marrow stromal cells (BMSCs). Although both peptide and agarose hydrogels retained TGF‐β1, fivefold higher retention was found in peptide. Excess unlabeled TGF‐β1 minimally displaced retained radiolabeled TGF‐β1, demonstrating biologically relevant loading capacity for peptide hydrogels. The initial release from acellular peptide hydrogels was nearly threefold lower than agarose hydrogels, at 18% of loaded TGF‐β1 through 3 days as compared to 48% for agarose. At day 21, cumulative release of TGF‐β1 was 32–44% from acellular peptide hydrogels, but was 62% from peptide hydrogels with encapsulated BMSCs, likely due to cell‐mediated TGF‐β1 degradation and release of small labeled species. TGF‐β1 loaded peptide hydrogels stimulated chondrogenesis of young equine BMSCs, a relevant preclinical model for treating injuries in young human cohorts. Self‐assembling peptide hydrogels can be used to deliver chondrogenic factors to encapsulated cells making them a promising technology for , cell‐based regenerative medicine. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1275–1285, 2014.
language:
source:
identifier: ISSN: 1549-3296 ; E-ISSN: 1552-4965 ; DOI: 10.1002/jbm.a.34789
fulltext: fulltext
issn:
  • 1549-3296
  • 15493296
  • 1552-4965
  • 15524965
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titleSustained delivery of bioactive TGF‐β1 from self‐assembling peptide hydrogels induces chondrogenesis of encapsulated bone marrow stromal cells
creatorKopesky, Paul W. ; Byun, Sangwon ; Vanderploeg, Eric J. ; Kisiday, John D. ; Frisbie, David D. ; Grodzinsky, Alan J.
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subjectTissue Engineering ; Sustained Delivery ; Bone Marrow Stromal Cell ; Regenerative Medicine ; Cartilage Repair
descriptionTissue engineering strategies for cartilage defect repair require technology for local targeted delivery of chondrogenic and anti‐inflammatory factors. The objective of this study was to determine the release kinetics of transforming growth factor β1 (TGF‐β1) from self‐assembling peptide hydrogels, a candidate scaffold for cell transplant therapies, and stimulate chondrogenesis of encapsulated young equine bone marrow stromal cells (BMSCs). Although both peptide and agarose hydrogels retained TGF‐β1, fivefold higher retention was found in peptide. Excess unlabeled TGF‐β1 minimally displaced retained radiolabeled TGF‐β1, demonstrating biologically relevant loading capacity for peptide hydrogels. The initial release from acellular peptide hydrogels was nearly threefold lower than agarose hydrogels, at 18% of loaded TGF‐β1 through 3 days as compared to 48% for agarose. At day 21, cumulative release of TGF‐β1 was 32–44% from acellular peptide hydrogels, but was 62% from peptide hydrogels with encapsulated BMSCs, likely due to cell‐mediated TGF‐β1 degradation and release of small labeled species. TGF‐β1 loaded peptide hydrogels stimulated chondrogenesis of young equine BMSCs, a relevant preclinical model for treating injuries in young human cohorts. Self‐assembling peptide hydrogels can be used to deliver chondrogenic factors to encapsulated cells making them a promising technology for , cell‐based regenerative medicine. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1275–1285, 2014.
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descriptionTissue engineering strategies for cartilage defect repair require technology for local targeted delivery of chondrogenic and anti‐inflammatory factors. The objective of this study was to determine the release kinetics of transforming growth factor β1 (TGF‐β1) from self‐assembling peptide hydrogels, a candidate scaffold for cell transplant therapies, and stimulate chondrogenesis of encapsulated young equine bone marrow stromal cells (BMSCs). Although both peptide and agarose hydrogels retained TGF‐β1, fivefold higher retention was found in peptide. Excess unlabeled TGF‐β1 minimally displaced retained radiolabeled TGF‐β1, demonstrating biologically relevant loading capacity for peptide hydrogels. The initial release from acellular peptide hydrogels was nearly threefold lower than agarose hydrogels, at 18% of loaded TGF‐β1 through 3 days as compared to 48% for agarose. At day 21, cumulative release of TGF‐β1 was 32–44% from acellular peptide hydrogels, but was 62% from peptide hydrogels with encapsulated BMSCs, likely due to cell‐mediated TGF‐β1 degradation and release of small labeled species. TGF‐β1 loaded peptide hydrogels stimulated chondrogenesis of young equine BMSCs, a relevant preclinical model for treating injuries in young human cohorts. Self‐assembling peptide hydrogels can be used to deliver chondrogenic factors to encapsulated cells making them a promising technology for , cell‐based regenerative medicine. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1275–1285, 2014.
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abstractTissue engineering strategies for cartilage defect repair require technology for local targeted delivery of chondrogenic and anti‐inflammatory factors. The objective of this study was to determine the release kinetics of transforming growth factor β1 (TGF‐β1) from self‐assembling peptide hydrogels, a candidate scaffold for cell transplant therapies, and stimulate chondrogenesis of encapsulated young equine bone marrow stromal cells (BMSCs). Although both peptide and agarose hydrogels retained TGF‐β1, fivefold higher retention was found in peptide. Excess unlabeled TGF‐β1 minimally displaced retained radiolabeled TGF‐β1, demonstrating biologically relevant loading capacity for peptide hydrogels. The initial release from acellular peptide hydrogels was nearly threefold lower than agarose hydrogels, at 18% of loaded TGF‐β1 through 3 days as compared to 48% for agarose. At day 21, cumulative release of TGF‐β1 was 32–44% from acellular peptide hydrogels, but was 62% from peptide hydrogels with encapsulated BMSCs, likely due to cell‐mediated TGF‐β1 degradation and release of small labeled species. TGF‐β1 loaded peptide hydrogels stimulated chondrogenesis of young equine BMSCs, a relevant preclinical model for treating injuries in young human cohorts. Self‐assembling peptide hydrogels can be used to deliver chondrogenic factors to encapsulated cells making them a promising technology for , cell‐based regenerative medicine. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1275–1285, 2014.
doi10.1002/jbm.a.34789
pages1275-1285
date2014-05