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Differentiation of embryonic stem cells to cardiomyocytes on electrospun nanofibrous substrates

The potential of pluripotent embryonic stem cells (ESCs) isolated from the inner mass of blastocysts are investigated for its ability to differentiate on biocompatible electrospun nanofibers, for regeneration of the myocardially infracted heart. Nanostructured poly(,‐lactide‐‐glycolide)/collagen (PL... Full description

Journal Title: Journal of Biomedical Materials Research Part B: Applied Biomaterials April 2014, Vol.102(3), pp.447-454
Main Author: Prabhakaran, Molamma P.
Other Authors: Mobarakeh, Laleh Ghasemi , Kai, Dan , Karbalaie, Khadijeh , Nasr‐Esfahani, Mohammad Hossein , Ramakrishna, Seeram
Format: Electronic Article Electronic Article
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ID: ISSN: 1552-4973 ; E-ISSN: 1552-4981 ; DOI: 10.1002/jbm.b.33022
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recordid: wj10.1002/jbm.b.33022
title: Differentiation of embryonic stem cells to cardiomyocytes on electrospun nanofibrous substrates
format: Article
creator:
  • Prabhakaran, Molamma P.
  • Mobarakeh, Laleh Ghasemi
  • Kai, Dan
  • Karbalaie, Khadijeh
  • Nasr‐Esfahani, Mohammad Hossein
  • Ramakrishna, Seeram
subjects:
  • Embryonic Stem Cells
  • Embryoid Bodies
  • Electrospinning
  • Cardiac Regeneration
  • Collagen
ispartof: Journal of Biomedical Materials Research Part B: Applied Biomaterials, April 2014, Vol.102(3), pp.447-454
description: The potential of pluripotent embryonic stem cells (ESCs) isolated from the inner mass of blastocysts are investigated for its ability to differentiate on biocompatible electrospun nanofibers, for regeneration of the myocardially infracted heart. Nanostructured poly(,‐lactide‐‐glycolide)/collagen (PLGA/Col) scaffolds with fiber diameters in the range of 300 ± 65 nm, was fabricated by electrospinning to mimic the extracellular matrix of the native tissue. During the culture of embryoid bodies outgrowth on the scaffolds, and further differentiation of ESCs to cardiomyocytes, the PLGA/Col nanofibers was found better than that of the electrospun PLGA nanofibers, where a better interaction and growth of ESC differentiated cardiomyocytes was observed on the composite scaffolds. The phenotypical characteristics of ESC‐derived cardiomyocytes and molecular protein expression were carried out by scanning electron microscopy and immunocytochemistry, respectively. Our studies highlight the significance of a suitable material, its architecture, and cell–biomaterial interactions that is essential at a nanoscale level signifying the application of a bioengineered cardiac graft for stem cell differentiation and transplantation, which could be an intriguing strategy for cardiac regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 447–454, 2014.
language:
source:
identifier: ISSN: 1552-4973 ; E-ISSN: 1552-4981 ; DOI: 10.1002/jbm.b.33022
fulltext: fulltext
issn:
  • 1552-4973
  • 15524973
  • 1552-4981
  • 15524981
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titleDifferentiation of embryonic stem cells to cardiomyocytes on electrospun nanofibrous substrates
creatorPrabhakaran, Molamma P. ; Mobarakeh, Laleh Ghasemi ; Kai, Dan ; Karbalaie, Khadijeh ; Nasr‐Esfahani, Mohammad Hossein ; Ramakrishna, Seeram
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subjectEmbryonic Stem Cells ; Embryoid Bodies ; Electrospinning ; Cardiac Regeneration ; Collagen
descriptionThe potential of pluripotent embryonic stem cells (ESCs) isolated from the inner mass of blastocysts are investigated for its ability to differentiate on biocompatible electrospun nanofibers, for regeneration of the myocardially infracted heart. Nanostructured poly(,‐lactide‐‐glycolide)/collagen (PLGA/Col) scaffolds with fiber diameters in the range of 300 ± 65 nm, was fabricated by electrospinning to mimic the extracellular matrix of the native tissue. During the culture of embryoid bodies outgrowth on the scaffolds, and further differentiation of ESCs to cardiomyocytes, the PLGA/Col nanofibers was found better than that of the electrospun PLGA nanofibers, where a better interaction and growth of ESC differentiated cardiomyocytes was observed on the composite scaffolds. The phenotypical characteristics of ESC‐derived cardiomyocytes and molecular protein expression were carried out by scanning electron microscopy and immunocytochemistry, respectively. Our studies highlight the significance of a suitable material, its architecture, and cell–biomaterial interactions that is essential at a nanoscale level signifying the application of a bioengineered cardiac graft for stem cell differentiation and transplantation, which could be an intriguing strategy for cardiac regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 447–454, 2014.
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descriptionThe potential of pluripotent embryonic stem cells (ESCs) isolated from the inner mass of blastocysts are investigated for its ability to differentiate on biocompatible electrospun nanofibers, for regeneration of the myocardially infracted heart. Nanostructured poly(,‐lactide‐‐glycolide)/collagen (PLGA/Col) scaffolds with fiber diameters in the range of 300 ± 65 nm, was fabricated by electrospinning to mimic the extracellular matrix of the native tissue. During the culture of embryoid bodies outgrowth on the scaffolds, and further differentiation of ESCs to cardiomyocytes, the PLGA/Col nanofibers was found better than that of the electrospun PLGA nanofibers, where a better interaction and growth of ESC differentiated cardiomyocytes was observed on the composite scaffolds. The phenotypical characteristics of ESC‐derived cardiomyocytes and molecular protein expression were carried out by scanning electron microscopy and immunocytochemistry, respectively. Our studies highlight the significance of a suitable material, its architecture, and cell–biomaterial interactions that is essential at a nanoscale level signifying the application of a bioengineered cardiac graft for stem cell differentiation and transplantation, which could be an intriguing strategy for cardiac regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 447–454, 2014.
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titleDifferentiation of embryonic stem cells to cardiomyocytes on electrospun nanofibrous substrates
authorPrabhakaran, Molamma P. ; Mobarakeh, Laleh Ghasemi ; Kai, Dan ; Karbalaie, Khadijeh ; Nasr‐Esfahani, Mohammad Hossein ; Ramakrishna, Seeram
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abstractThe potential of pluripotent embryonic stem cells (ESCs) isolated from the inner mass of blastocysts are investigated for its ability to differentiate on biocompatible electrospun nanofibers, for regeneration of the myocardially infracted heart. Nanostructured poly(,‐lactide‐‐glycolide)/collagen (PLGA/Col) scaffolds with fiber diameters in the range of 300 ± 65 nm, was fabricated by electrospinning to mimic the extracellular matrix of the native tissue. During the culture of embryoid bodies outgrowth on the scaffolds, and further differentiation of ESCs to cardiomyocytes, the PLGA/Col nanofibers was found better than that of the electrospun PLGA nanofibers, where a better interaction and growth of ESC differentiated cardiomyocytes was observed on the composite scaffolds. The phenotypical characteristics of ESC‐derived cardiomyocytes and molecular protein expression were carried out by scanning electron microscopy and immunocytochemistry, respectively. Our studies highlight the significance of a suitable material, its architecture, and cell–biomaterial interactions that is essential at a nanoscale level signifying the application of a bioengineered cardiac graft for stem cell differentiation and transplantation, which could be an intriguing strategy for cardiac regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 447–454, 2014.
doi10.1002/jbm.b.33022
pages447-454
date2014-04