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Purification of Lactobacillus acidophilus surface‐layer protein and its immunomodulatory effects on RAW264.7 cells

Byline: Dandan Zhang, Mengting Wu, Yuxing Guo, Mingyue Xun, Wenwen Wang, Zhen Wu, Daodong Pan Keywords: immunomodulatory capacity; surface-layer protein; purification; Lactobacillus acidophilus Abstract BACKGROUND Surface-layer proteins (SLP) have been found in the outermost layer of the cell wall i... Full description

Journal Title: Journal of the Science of Food and Agriculture September 2017, Vol.97(12), pp.4204-4209
Main Author: Zhang, Dandan
Other Authors: Wu, Mengting , Guo, Yuxing , Xun, Mingyue , Wang, Wenwen , Wu, Zhen , Pan, Daodong
Format: Electronic Article Electronic Article
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ID: ISSN: 0022-5142 ; E-ISSN: 1097-0010 ; DOI: 10.1002/jsfa.8294
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recordid: wj10.1002/jsfa.8294
title: Purification of Lactobacillus acidophilus surface‐layer protein and its immunomodulatory effects on RAW264.7 cells
format: Article
creator:
  • Zhang, Dandan
  • Wu, Mengting
  • Guo, Yuxing
  • Xun, Mingyue
  • Wang, Wenwen
  • Wu, Zhen
  • Pan, Daodong
subjects:
  • Immunomodulatory Capacity
  • Surface‐Layer Protein
  • Purification
  • Lactobacillus Acidophilus
ispartof: Journal of the Science of Food and Agriculture, September 2017, Vol.97(12), pp.4204-4209
description: Byline: Dandan Zhang, Mengting Wu, Yuxing Guo, Mingyue Xun, Wenwen Wang, Zhen Wu, Daodong Pan Keywords: immunomodulatory capacity; surface-layer protein; purification; Lactobacillus acidophilus Abstract BACKGROUND Surface-layer proteins (SLP) have been found in the outermost layer of the cell wall in many types of lactobacillus are considered to be an important factor with respect to intestinal immunity. RESULTS The present study compared the effects of SLP extracted by different concentrations of LiCl and carbamide, and subsequently identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, circular dichroism and differential scanning calorimetry. Furthermore, RAW 264.7 cells were used to evaluate the immunomodulatory effects of SLP. SLP were derived from Lactobacillus acidophilus CICC6074 with a molecular weight of 46kDa, and consisted of 16.9% [alpha]-helix, 42.3% [beta]-sheet, 20.8% [beta]-turns and 22.5% random coils. SLP promoted NO secretion and higher quantities of NO were produced as the SLP concentrations increased. SLP concentrations over 50A[micro]gmL.sub.-1 significantly decreased the amount of tumor necrosis factor-[alpha] secreted by RAW264.7 cells. CONCLUSION SLP can trigger immunomodulatory effects in RAW 264.7 cells. This provides crucial information that will enable the further use of L.acidophilus in food, medicine and other products. [c] 2017 Society of Chemical Industry CAPTION(S): Figure S1. SLP identified by the MALDI-TOF/TOF analysis.
language:
source:
identifier: ISSN: 0022-5142 ; E-ISSN: 1097-0010 ; DOI: 10.1002/jsfa.8294
fulltext: fulltext
issn:
  • 0022-5142
  • 00225142
  • 1097-0010
  • 10970010
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titlePurification of Lactobacillus acidophilus surface‐layer protein and its immunomodulatory effects on RAW264.7 cells
creatorZhang, Dandan ; Wu, Mengting ; Guo, Yuxing ; Xun, Mingyue ; Wang, Wenwen ; Wu, Zhen ; Pan, Daodong
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descriptionByline: Dandan Zhang, Mengting Wu, Yuxing Guo, Mingyue Xun, Wenwen Wang, Zhen Wu, Daodong Pan Keywords: immunomodulatory capacity; surface-layer protein; purification; Lactobacillus acidophilus Abstract BACKGROUND Surface-layer proteins (SLP) have been found in the outermost layer of the cell wall in many types of lactobacillus are considered to be an important factor with respect to intestinal immunity. RESULTS The present study compared the effects of SLP extracted by different concentrations of LiCl and carbamide, and subsequently identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, circular dichroism and differential scanning calorimetry. Furthermore, RAW 264.7 cells were used to evaluate the immunomodulatory effects of SLP. SLP were derived from Lactobacillus acidophilus CICC6074 with a molecular weight of 46kDa, and consisted of 16.9% [alpha]-helix, 42.3% [beta]-sheet, 20.8% [beta]-turns and 22.5% random coils. SLP promoted NO secretion and higher quantities of NO were produced as the SLP concentrations increased. SLP concentrations over 50A[micro]gmL.sub.-1 significantly decreased the amount of tumor necrosis factor-[alpha] secreted by RAW264.7 cells. CONCLUSION SLP can trigger immunomodulatory effects in RAW 264.7 cells. This provides crucial information that will enable the further use of L.acidophilus in food, medicine and other products. [c] 2017 Society of Chemical Industry CAPTION(S): Figure S1. SLP identified by the MALDI-TOF/TOF analysis.
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