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Transepithelial transport of milk‐derived angiotensin I‐converting enzyme inhibitory peptide with the RLSFNP sequence

To purchase or authenticate to the full-text of this article, please visit this link: http://onlinelibrary.wiley.com/doi/10.1002/jsfa.8545/abstract Byline: Yuxing Guo, Junai Gan, Qian Zhu, Xiaoqun Zeng, Yangying Sun, Zhen Wu,Daodong Pan Keywords: ACE-inhibitory peptide; Caco-2 cell monolayer; transe... Full description

Journal Title: Journal of the Science of Food and Agriculture February 2018, Vol.98(3), pp.976-983
Main Author: Guo, Yuxing
Other Authors: Gan, Junai , Zhu, Qian , Zeng, Xiaoqun , Sun, Yangying , Wu, Zhen , Pan, Daodong
Format: Electronic Article Electronic Article
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ID: ISSN: 0022-5142 ; E-ISSN: 1097-0010 ; DOI: 10.1002/jsfa.8545
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recordid: wj10.1002/jsfa.8545
title: Transepithelial transport of milk‐derived angiotensin I‐converting enzyme inhibitory peptide with the RLSFNP sequence
format: Article
creator:
  • Guo, Yuxing
  • Gan, Junai
  • Zhu, Qian
  • Zeng, Xiaoqun
  • Sun, Yangying
  • Wu, Zhen
  • Pan, Daodong
subjects:
  • Ace‐Inhibitory Peptide
  • Caco‐2 Cell Monolayer
  • Transepithelial Transport
  • Paracellular Transport
  • Permeability
ispartof: Journal of the Science of Food and Agriculture, February 2018, Vol.98(3), pp.976-983
description: To purchase or authenticate to the full-text of this article, please visit this link: http://onlinelibrary.wiley.com/doi/10.1002/jsfa.8545/abstract Byline: Yuxing Guo, Junai Gan, Qian Zhu, Xiaoqun Zeng, Yangying Sun, Zhen Wu,Daodong Pan Keywords: ACE-inhibitory peptide; Caco-2 cell monolayer; transepithelial transport; paracellular transport; permeability Abstract BACKGROUND To exert an antihypertensive effect after oral administration, angiotensin I-converting enzyme (ACE)-inhibitory peptides must remain active after intestinal transport. The purpose of this article is to elucidate the transport permeability and route of ACE-inhibitory peptide Arg-Leu-Ser-Phe-Asn-Pro (RLSFNP) across the intestinal epithelium using Caco-2 cell monolayers. RESULTS Intact RLSFNP and RLSFNP breakdown fragments F, FNP, SFNP and RLSF were found in RLSFNP transport solution across Caco-2 cell monolayers using ultra-performance liquid chromatography-tandem mass spectrometry. RLSFNP fragments FNP, SFNP and RLSF also contributed to ACE inhibitory effects. Protease inhibitors (bacitracin and leupeptin) and absorption enhancers (sodium glycocholate hydrate, sodium deoxycholate and Na.sub.2EDTA) improved the transport flux of RLSFNP. A transport inhibitor experiment showed that intact RLSFNP may be transported via the paracellular route. CONCLUSION Intact RLSFNP can be transported across the Caco-2 cell monolayers via the paracellular route. Extensive hydrolysis was the chief reason for the low permeability of RLSFNP. [c] 2017 Society of Chemical Industry
language:
source:
identifier: ISSN: 0022-5142 ; E-ISSN: 1097-0010 ; DOI: 10.1002/jsfa.8545
fulltext: fulltext
issn:
  • 0022-5142
  • 00225142
  • 1097-0010
  • 10970010
url: Link


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titleTransepithelial transport of milk‐derived angiotensin I‐converting enzyme inhibitory peptide with the RLSFNP sequence
creatorGuo, Yuxing ; Gan, Junai ; Zhu, Qian ; Zeng, Xiaoqun ; Sun, Yangying ; Wu, Zhen ; Pan, Daodong
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subjectAce‐Inhibitory Peptide ; Caco‐2 Cell Monolayer ; Transepithelial Transport ; Paracellular Transport ; Permeability
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descriptionTo purchase or authenticate to the full-text of this article, please visit this link: http://onlinelibrary.wiley.com/doi/10.1002/jsfa.8545/abstract Byline: Yuxing Guo, Junai Gan, Qian Zhu, Xiaoqun Zeng, Yangying Sun, Zhen Wu,Daodong Pan Keywords: ACE-inhibitory peptide; Caco-2 cell monolayer; transepithelial transport; paracellular transport; permeability Abstract BACKGROUND To exert an antihypertensive effect after oral administration, angiotensin I-converting enzyme (ACE)-inhibitory peptides must remain active after intestinal transport. The purpose of this article is to elucidate the transport permeability and route of ACE-inhibitory peptide Arg-Leu-Ser-Phe-Asn-Pro (RLSFNP) across the intestinal epithelium using Caco-2 cell monolayers. RESULTS Intact RLSFNP and RLSFNP breakdown fragments F, FNP, SFNP and RLSF were found in RLSFNP transport solution across Caco-2 cell monolayers using ultra-performance liquid chromatography-tandem mass spectrometry. RLSFNP fragments FNP, SFNP and RLSF also contributed to ACE inhibitory effects. Protease inhibitors (bacitracin and leupeptin) and absorption enhancers (sodium glycocholate hydrate, sodium deoxycholate and Na.sub.2EDTA) improved the transport flux of RLSFNP. A transport inhibitor experiment showed that intact RLSFNP may be transported via the paracellular route. CONCLUSION Intact RLSFNP can be transported across the Caco-2 cell monolayers via the paracellular route. Extensive hydrolysis was the chief reason for the low permeability of RLSFNP. [c] 2017 Society of Chemical Industry
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titleTransepithelial transport of milk‐derived angiotensin I‐converting enzyme inhibitory peptide with the RLSFNP sequence
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