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In vitro antiprotozoan activity and mechanisms of action of selected Ghanaian medicinal plants against Trypanosoma, Leishmania, and Plasmodium parasites

Trypanosomiasis, leishmaniasis, and malaria are protozoan infections of public health importance with thousands of new cases recorded annually. Control of these infection(s) with existing chemotherapy is limited by drug toxicity, lengthy parenteral treatment, affordability, and/or the emergence of r... Full description

Journal Title: Phytotherapy Research August 2018, Vol.32(8), pp.1617-1630
Main Author: Ohashi, Mitsuko
Other Authors: Amoa - Bosompem, Michael , Kwofie, Kofi Dadzie , Agyapong, Jefferey , Adegle, Richard , Sakyiamah, Maxwell Mamfe , Ayertey, Frederick , Owusu, Kofi Baffuor‐Awuah , Tuffour, Isaac , Atchoglo, Philip , Tung, Nguyen Huu , Uto, Takuhiro , Aboagye, Frederick , Appiah, Alfred Ampomah , Appiah - Opong, Regina , Nyarko, Alexander K. , Anyan, William Kofi , Ayi, Irene , Boakye, Daniel Adjei , Koram, Kwadwo Ansah , Edoh, Dominic , Yamaoka, Shoji , Shoyama, Yukihiro , Ohta, Nobuo
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ID: ISSN: 0951-418X ; E-ISSN: 1099-1573 ; DOI: 10.1002/ptr.6093
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title: In vitro antiprotozoan activity and mechanisms of action of selected Ghanaian medicinal plants against Trypanosoma, Leishmania, and Plasmodium parasites
format: Article
creator:
  • Ohashi, Mitsuko
  • Amoa - Bosompem, Michael
  • Kwofie, Kofi Dadzie
  • Agyapong, Jefferey
  • Adegle, Richard
  • Sakyiamah, Maxwell Mamfe
  • Ayertey, Frederick
  • Owusu, Kofi Baffuor‐Awuah
  • Tuffour, Isaac
  • Atchoglo, Philip
  • Tung, Nguyen Huu
  • Uto, Takuhiro
  • Aboagye, Frederick
  • Appiah, Alfred Ampomah
  • Appiah - Opong, Regina
  • Nyarko, Alexander K.
  • Anyan, William Kofi
  • Ayi, Irene
  • Boakye, Daniel Adjei
  • Koram, Kwadwo Ansah
  • Edoh, Dominic
  • Yamaoka, Shoji
  • Shoyama, Yukihiro
  • Ohta, Nobuo
subjects:
  • Apoptosis
  • In Vitro Screening
  • Leishmania Donovani
  • Medicinal Plants
  • Morphology
  • Plasmodium Falciparum
  • Trypanosoma Brucei Brucei
ispartof: Phytotherapy Research, August 2018, Vol.32(8), pp.1617-1630
description: Trypanosomiasis, leishmaniasis, and malaria are protozoan infections of public health importance with thousands of new cases recorded annually. Control of these infection(s) with existing chemotherapy is limited by drug toxicity, lengthy parenteral treatment, affordability, and/or the emergence of resistant strains. Medicinal plants on the other hand are used in the treatment of various infectious diseases although their chemical properties are not fully evaluated. In this study, we screened 112 crude extracts from 72 selected Ghanaian medicinal plants for anti‐, anti‐, and anti‐ activities in vitro and investigated their mechanisms of action. Twenty‐three extracts from 20 plants showed significant antiprotozoan activity against at least 1 of 3 protozoan parasites screened with IC values less than 20 μg/ml. Eleven extracts showed high anti‐ activity with whole plant and leaf extracts recording the highest activities. Their IC (selectivity index [SI]) values were 5.51 μg/ml (35.00) and 5.96 μg/ml (13.09), respectively. Nine extracts had high anti‐ activity with and leaf extracts as the most active. Their IC (SI) values were 10.8 μg/ml (1.50) and 10.1 μg/ml (0.37), respectively. Six extracts had high anti‐ activity with the leaf and stem‐bark extracts of recording the highest activity. Their IC (SI) values were 7.26 μg/ml (129.36) and 17.45 μg/ml (17.17), respectively. Only at 25 μg/ml induced significant apoptosis‐like cell death in parasites. Anti‐ active extracts induced varying morphological changes in parasites such as multiple nuclei and/or kinetoplast, incomplete flagella division, or nuclear fragmentation. Active extracts may be potential sources for developing new chemotherapy against these infections.
language:
source:
identifier: ISSN: 0951-418X ; E-ISSN: 1099-1573 ; DOI: 10.1002/ptr.6093
fulltext: fulltext
issn:
  • 0951-418X
  • 0951418X
  • 1099-1573
  • 10991573
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titleIn vitro antiprotozoan activity and mechanisms of action of selected Ghanaian medicinal plants against Trypanosoma, Leishmania, and Plasmodium parasites
creatorOhashi, Mitsuko ; Amoa - Bosompem, Michael ; Kwofie, Kofi Dadzie ; Agyapong, Jefferey ; Adegle, Richard ; Sakyiamah, Maxwell Mamfe ; Ayertey, Frederick ; Owusu, Kofi Baffuor‐Awuah ; Tuffour, Isaac ; Atchoglo, Philip ; Tung, Nguyen Huu ; Uto, Takuhiro ; Aboagye, Frederick ; Appiah, Alfred Ampomah ; Appiah - Opong, Regina ; Nyarko, Alexander K. ; Anyan, William Kofi ; Ayi, Irene ; Boakye, Daniel Adjei ; Koram, Kwadwo Ansah ; Edoh, Dominic ; Yamaoka, Shoji ; Shoyama, Yukihiro ; Ohta, Nobuo
ispartofPhytotherapy Research, August 2018, Vol.32(8), pp.1617-1630
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subjectApoptosis ; In Vitro Screening ; Leishmania Donovani ; Medicinal Plants ; Morphology ; Plasmodium Falciparum ; Trypanosoma Brucei Brucei
descriptionTrypanosomiasis, leishmaniasis, and malaria are protozoan infections of public health importance with thousands of new cases recorded annually. Control of these infection(s) with existing chemotherapy is limited by drug toxicity, lengthy parenteral treatment, affordability, and/or the emergence of resistant strains. Medicinal plants on the other hand are used in the treatment of various infectious diseases although their chemical properties are not fully evaluated. In this study, we screened 112 crude extracts from 72 selected Ghanaian medicinal plants for anti‐, anti‐, and anti‐ activities in vitro and investigated their mechanisms of action. Twenty‐three extracts from 20 plants showed significant antiprotozoan activity against at least 1 of 3 protozoan parasites screened with IC values less than 20 μg/ml. Eleven extracts showed high anti‐ activity with whole plant and leaf extracts recording the highest activities. Their IC (selectivity index [SI]) values were 5.51 μg/ml (35.00) and 5.96 μg/ml (13.09), respectively. Nine extracts had high anti‐ activity with and leaf extracts as the most active. Their IC (SI) values were 10.8 μg/ml (1.50) and 10.1 μg/ml (0.37), respectively. Six extracts had high anti‐ activity with the leaf and stem‐bark extracts of recording the highest activity. Their IC (SI) values were 7.26 μg/ml (129.36) and 17.45 μg/ml (17.17), respectively. Only at 25 μg/ml induced significant apoptosis‐like cell death in parasites. Anti‐ active extracts induced varying morphological changes in parasites such as multiple nuclei and/or kinetoplast, incomplete flagella division, or nuclear fragmentation. Active extracts may be potential sources for developing new chemotherapy against these infections.
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titleIn vitro antiprotozoan activity and mechanisms of action of selected Ghanaian medicinal plants against Trypanosoma, Leishmania, and Plasmodium parasites
descriptionTrypanosomiasis, leishmaniasis, and malaria are protozoan infections of public health importance with thousands of new cases recorded annually. Control of these infection(s) with existing chemotherapy is limited by drug toxicity, lengthy parenteral treatment, affordability, and/or the emergence of resistant strains. Medicinal plants on the other hand are used in the treatment of various infectious diseases although their chemical properties are not fully evaluated. In this study, we screened 112 crude extracts from 72 selected Ghanaian medicinal plants for anti‐, anti‐, and anti‐ activities in vitro and investigated their mechanisms of action. Twenty‐three extracts from 20 plants showed significant antiprotozoan activity against at least 1 of 3 protozoan parasites screened with IC values less than 20 μg/ml. Eleven extracts showed high anti‐ activity with whole plant and leaf extracts recording the highest activities. Their IC (selectivity index [SI]) values were 5.51 μg/ml (35.00) and 5.96 μg/ml (13.09), respectively. Nine extracts had high anti‐ activity with and leaf extracts as the most active. Their IC (SI) values were 10.8 μg/ml (1.50) and 10.1 μg/ml (0.37), respectively. Six extracts had high anti‐ activity with the leaf and stem‐bark extracts of recording the highest activity. Their IC (SI) values were 7.26 μg/ml (129.36) and 17.45 μg/ml (17.17), respectively. Only at 25 μg/ml induced significant apoptosis‐like cell death in parasites. Anti‐ active extracts induced varying morphological changes in parasites such as multiple nuclei and/or kinetoplast, incomplete flagella division, or nuclear fragmentation. Active extracts may be potential sources for developing new chemotherapy against these infections.
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titleIn vitro antiprotozoan activity and mechanisms of action of selected Ghanaian medicinal plants against Trypanosoma, Leishmania, and Plasmodium parasites
authorOhashi, Mitsuko ; Amoa - Bosompem, Michael ; Kwofie, Kofi Dadzie ; Agyapong, Jefferey ; Adegle, Richard ; Sakyiamah, Maxwell Mamfe ; Ayertey, Frederick ; Owusu, Kofi Baffuor‐Awuah ; Tuffour, Isaac ; Atchoglo, Philip ; Tung, Nguyen Huu ; Uto, Takuhiro ; Aboagye, Frederick ; Appiah, Alfred Ampomah ; Appiah - Opong, Regina ; Nyarko, Alexander K. ; Anyan, William Kofi ; Ayi, Irene ; Boakye, Daniel Adjei ; Koram, Kwadwo Ansah ; Edoh, Dominic ; Yamaoka, Shoji ; Shoyama, Yukihiro ; Ohta, Nobuo
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atitleIn vitro antiprotozoan activity and mechanisms of action of selected Ghanaian medicinal plants against Trypanosoma, Leishmania, and Plasmodium parasites
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abstractTrypanosomiasis, leishmaniasis, and malaria are protozoan infections of public health importance with thousands of new cases recorded annually. Control of these infection(s) with existing chemotherapy is limited by drug toxicity, lengthy parenteral treatment, affordability, and/or the emergence of resistant strains. Medicinal plants on the other hand are used in the treatment of various infectious diseases although their chemical properties are not fully evaluated. In this study, we screened 112 crude extracts from 72 selected Ghanaian medicinal plants for anti‐, anti‐, and anti‐ activities in vitro and investigated their mechanisms of action. Twenty‐three extracts from 20 plants showed significant antiprotozoan activity against at least 1 of 3 protozoan parasites screened with IC values less than 20 μg/ml. Eleven extracts showed high anti‐ activity with whole plant and leaf extracts recording the highest activities. Their IC (selectivity index [SI]) values were 5.51 μg/ml (35.00) and 5.96 μg/ml (13.09), respectively. Nine extracts had high anti‐ activity with and leaf extracts as the most active. Their IC (SI) values were 10.8 μg/ml (1.50) and 10.1 μg/ml (0.37), respectively. Six extracts had high anti‐ activity with the leaf and stem‐bark extracts of recording the highest activity. Their IC (SI) values were 7.26 μg/ml (129.36) and 17.45 μg/ml (17.17), respectively. Only at 25 μg/ml induced significant apoptosis‐like cell death in parasites. Anti‐ active extracts induced varying morphological changes in parasites such as multiple nuclei and/or kinetoplast, incomplete flagella division, or nuclear fragmentation. Active extracts may be potential sources for developing new chemotherapy against these infections.
doi10.1002/ptr.6093
pages1617-1630
orcidid0000-0001-9329-7920
date2018-08