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GPX3 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X‐ray analysis

The glutathione peroxidase 3 (GPX3) gene encodes a glutathione peroxidase with roles in HO homeostasis and signalling. The GPX3 gene sequence was cloned into pGEX‐6P1 and overexpressed in . The GPX3 protein was purified to homogeneity in two chromatographic steps. Various lengths of the GPX3 sequenc... Full description

Journal Title: Acta Crystallographica Section F 01 November 2013, Vol.69(11), pp.1224-1226
Main Author: Li, Kun
Other Authors: Yang, Qingzhan , Wang, Wei , Zhao, Xiaoliang , Lou, Zhiyong
Format: Electronic Article Electronic Article
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ID: ISSN: 1744-3091 ; E-ISSN: 1744-3091 ; DOI: 10.1107/S1744309113025566
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recordid: wj10.1107/S1744309113025566
title: GPX3 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X‐ray analysis
format: Article
creator:
  • Li, Kun
  • Yang, Qingzhan
  • Wang, Wei
  • Zhao, Xiaoliang
  • Lou, Zhiyong
subjects:
  • Glutathione Peroxidase 3
  • Gpx3
  • Arabidopsis Thaliana
ispartof: Acta Crystallographica Section F, 01 November 2013, Vol.69(11), pp.1224-1226
description: The glutathione peroxidase 3 (GPX3) gene encodes a glutathione peroxidase with roles in HO homeostasis and signalling. The GPX3 gene sequence was cloned into pGEX‐6P1 and overexpressed in . The GPX3 protein was purified to homogeneity in two chromatographic steps. Various lengths of the GPX3 sequence were used to obtain proteins that yielded crystals using vapour‐diffusion techniques, but only GPX3ΔN36 (lacking 36 amino acids from the N‐terminus) showed a good diffraction pattern. Its crystals diffracted to 2.8 Å resolution and belonged to space group 6, with unit‐cell parameters = = 98.241, = 42.057 Å.
language:
source:
identifier: ISSN: 1744-3091 ; E-ISSN: 1744-3091 ; DOI: 10.1107/S1744309113025566
fulltext: fulltext
issn:
  • 1744-3091
  • 17443091
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titleGPX3 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X‐ray analysis
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subjectGlutathione Peroxidase 3 ; Gpx3 ; Arabidopsis Thaliana
descriptionThe glutathione peroxidase 3 (GPX3) gene encodes a glutathione peroxidase with roles in HO homeostasis and signalling. The GPX3 gene sequence was cloned into pGEX‐6P1 and overexpressed in . The GPX3 protein was purified to homogeneity in two chromatographic steps. Various lengths of the GPX3 sequence were used to obtain proteins that yielded crystals using vapour‐diffusion techniques, but only GPX3ΔN36 (lacking 36 amino acids from the N‐terminus) showed a good diffraction pattern. Its crystals diffracted to 2.8 Å resolution and belonged to space group 6, with unit‐cell parameters = = 98.241, = 42.057 Å.
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titleGPX3 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X‐ray analysis
descriptionThe glutathione peroxidase 3 (GPX3) gene encodes a glutathione peroxidase with roles in HO homeostasis and signalling. The GPX3 gene sequence was cloned into pGEX‐6P1 and overexpressed in . The GPX3 protein was purified to homogeneity in two chromatographic steps. Various lengths of the GPX3 sequence were used to obtain proteins that yielded crystals using vapour‐diffusion techniques, but only GPX3ΔN36 (lacking 36 amino acids from the N‐terminus) showed a good diffraction pattern. Its crystals diffracted to 2.8 Å resolution and belonged to space group 6, with unit‐cell parameters = = 98.241, = 42.057 Å.
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abstractThe glutathione peroxidase 3 (GPX3) gene encodes a glutathione peroxidase with roles in HO homeostasis and signalling. The GPX3 gene sequence was cloned into pGEX‐6P1 and overexpressed in . The GPX3 protein was purified to homogeneity in two chromatographic steps. Various lengths of the GPX3 sequence were used to obtain proteins that yielded crystals using vapour‐diffusion techniques, but only GPX3ΔN36 (lacking 36 amino acids from the N‐terminus) showed a good diffraction pattern. Its crystals diffracted to 2.8 Å resolution and belonged to space group 6, with unit‐cell parameters = = 98.241, = 42.057 Å.
cop5 Abbey Square, Chester, Cheshire CH1 2HU, England
pubInternational Union of Crystallography
doi10.1107/S1744309113025566
pages1224-1226
date2013-11-01