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Silencing of odorant binding protein gene AlinOBP4 by RNAi induces declining electrophysiological responses of Adelphocoris lineolatus to six semiochemicals

Odorant binding proteins (OBPs) are believed to be important for transporting semiochemicals through the aqueous sensillar lymph to the olfactory receptor cells within the insect antennal sensilla. Here, we injected ‐siRNA into the conjunctivum between prothorax and mesothorax of the lucerne plant b... Full description

Journal Title: Insect Science October 2017, Vol.24(5), pp.789-797
Main Author: Zhang, Xue‐Ying
Other Authors: Zhu, Xiao‐Qiang , Gu, Shao‐Hua , Zhou, Yan‐Le , Wang, Song‐Ying , Zhang, Yong‐Jun , Guo, Yu‐Yuan
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ID: ISSN: 1672-9609 ; E-ISSN: 1744-7917 ; DOI: 10.1111/1744-7917.12365
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title: Silencing of odorant binding protein gene AlinOBP4 by RNAi induces declining electrophysiological responses of Adelphocoris lineolatus to six semiochemicals
format: Article
creator:
  • Zhang, Xue‐Ying
  • Zhu, Xiao‐Qiang
  • Gu, Shao‐Hua
  • Zhou, Yan‐Le
  • Wang, Song‐Ying
  • Zhang, Yong‐Jun
  • Guo, Yu‐Yuan
subjects:
  • Adelphocoris Lineolatus
  • Electrophysiological Responses
  • Odorant Binding Protein Alinobp4
  • Rna Interference
ispartof: Insect Science, October 2017, Vol.24(5), pp.789-797
description: Odorant binding proteins (OBPs) are believed to be important for transporting semiochemicals through the aqueous sensillar lymph to the olfactory receptor cells within the insect antennal sensilla. Here, we injected ‐siRNA into the conjunctivum between prothorax and mesothorax of the lucerne plant bug, and evaluated the silencing of by reverse transcription polymerase chain reaction (RT‐PCR) analysis, quantitative real‐time PCR (qPCR) test and electroantennogram (EAG) assay. The combination of RT‐PCR and qPCR analyses revealed that the levels of messenger RNA transcript were significantly reduced ∼95% in ‐siRNA‐treated males and ∼75% in RNAi‐treated females within 48 hours. It was found that there are different EAG responses between male and female bugs when the gene was silenced by RNAi. The EAGs of to two plant volatiles, tridecanal and hexyl alcohol, were reduced 9.09% and 79.45% in RNAi‐treated males, 62.08% and 62.08% in RNAi‐treated females compared to the controls, separately. Antennae of RNAi‐treated bugs showed significantly lower electrophysiological responses to four sex pheromone analogs, butyl butanoate, 1‐hexyl butyrate, ()‐2‐hexenyl butyrate and hexyl hexanoate. The EAG recordings were reduced 35.43%, 35.24%, 39.96% and 78.47% in RNAi‐treated males and 64.52%, 18.13%, 36.88% and 49.52% in RNAi‐treated females, respectively. The results suggested that AlinOBP4 might play dual‐roles in the identification of plant volatiles and sex pheromones. It was suspected that AlinOBP4 may have different functions in odor perception between male and female .
language:
source:
identifier: ISSN: 1672-9609 ; E-ISSN: 1744-7917 ; DOI: 10.1111/1744-7917.12365
fulltext: fulltext
issn:
  • 1672-9609
  • 16729609
  • 1744-7917
  • 17447917
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titleSilencing of odorant binding protein gene AlinOBP4 by RNAi induces declining electrophysiological responses of Adelphocoris lineolatus to six semiochemicals
creatorZhang, Xue‐Ying ; Zhu, Xiao‐Qiang ; Gu, Shao‐Hua ; Zhou, Yan‐Le ; Wang, Song‐Ying ; Zhang, Yong‐Jun ; Guo, Yu‐Yuan
ispartofInsect Science, October 2017, Vol.24(5), pp.789-797
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subjectAdelphocoris Lineolatus ; Electrophysiological Responses ; Odorant Binding Protein Alinobp4 ; Rna Interference
descriptionOdorant binding proteins (OBPs) are believed to be important for transporting semiochemicals through the aqueous sensillar lymph to the olfactory receptor cells within the insect antennal sensilla. Here, we injected ‐siRNA into the conjunctivum between prothorax and mesothorax of the lucerne plant bug, and evaluated the silencing of by reverse transcription polymerase chain reaction (RT‐PCR) analysis, quantitative real‐time PCR (qPCR) test and electroantennogram (EAG) assay. The combination of RT‐PCR and qPCR analyses revealed that the levels of messenger RNA transcript were significantly reduced ∼95% in ‐siRNA‐treated males and ∼75% in RNAi‐treated females within 48 hours. It was found that there are different EAG responses between male and female bugs when the gene was silenced by RNAi. The EAGs of to two plant volatiles, tridecanal and hexyl alcohol, were reduced 9.09% and 79.45% in RNAi‐treated males, 62.08% and 62.08% in RNAi‐treated females compared to the controls, separately. Antennae of RNAi‐treated bugs showed significantly lower electrophysiological responses to four sex pheromone analogs, butyl butanoate, 1‐hexyl butyrate, ()‐2‐hexenyl butyrate and hexyl hexanoate. The EAG recordings were reduced 35.43%, 35.24%, 39.96% and 78.47% in RNAi‐treated males and 64.52%, 18.13%, 36.88% and 49.52% in RNAi‐treated females, respectively. The results suggested that AlinOBP4 might play dual‐roles in the identification of plant volatiles and sex pheromones. It was suspected that AlinOBP4 may have different functions in odor perception between male and female .
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titleSilencing of odorant binding protein gene AlinOBP4 by RNAi induces declining electrophysiological responses of Adelphocoris lineolatus to six semiochemicals
descriptionOdorant binding proteins (OBPs) are believed to be important for transporting semiochemicals through the aqueous sensillar lymph to the olfactory receptor cells within the insect antennal sensilla. Here, we injected ‐siRNA into the conjunctivum between prothorax and mesothorax of the lucerne plant bug, and evaluated the silencing of by reverse transcription polymerase chain reaction (RT‐PCR) analysis, quantitative real‐time PCR (qPCR) test and electroantennogram (EAG) assay. The combination of RT‐PCR and qPCR analyses revealed that the levels of messenger RNA transcript were significantly reduced ∼95% in ‐siRNA‐treated males and ∼75% in RNAi‐treated females within 48 hours. It was found that there are different EAG responses between male and female bugs when the gene was silenced by RNAi. The EAGs of to two plant volatiles, tridecanal and hexyl alcohol, were reduced 9.09% and 79.45% in RNAi‐treated males, 62.08% and 62.08% in RNAi‐treated females compared to the controls, separately. Antennae of RNAi‐treated bugs showed significantly lower electrophysiological responses to four sex pheromone analogs, butyl butanoate, 1‐hexyl butyrate, ()‐2‐hexenyl butyrate and hexyl hexanoate. The EAG recordings were reduced 35.43%, 35.24%, 39.96% and 78.47% in RNAi‐treated males and 64.52%, 18.13%, 36.88% and 49.52% in RNAi‐treated females, respectively. The results suggested that AlinOBP4 might play dual‐roles in the identification of plant volatiles and sex pheromones. It was suspected that AlinOBP4 may have different functions in odor perception between male and female .
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abstractOdorant binding proteins (OBPs) are believed to be important for transporting semiochemicals through the aqueous sensillar lymph to the olfactory receptor cells within the insect antennal sensilla. Here, we injected ‐siRNA into the conjunctivum between prothorax and mesothorax of the lucerne plant bug, and evaluated the silencing of by reverse transcription polymerase chain reaction (RT‐PCR) analysis, quantitative real‐time PCR (qPCR) test and electroantennogram (EAG) assay. The combination of RT‐PCR and qPCR analyses revealed that the levels of messenger RNA transcript were significantly reduced ∼95% in ‐siRNA‐treated males and ∼75% in RNAi‐treated females within 48 hours. It was found that there are different EAG responses between male and female bugs when the gene was silenced by RNAi. The EAGs of to two plant volatiles, tridecanal and hexyl alcohol, were reduced 9.09% and 79.45% in RNAi‐treated males, 62.08% and 62.08% in RNAi‐treated females compared to the controls, separately. Antennae of RNAi‐treated bugs showed significantly lower electrophysiological responses to four sex pheromone analogs, butyl butanoate, 1‐hexyl butyrate, ()‐2‐hexenyl butyrate and hexyl hexanoate. The EAG recordings were reduced 35.43%, 35.24%, 39.96% and 78.47% in RNAi‐treated males and 64.52%, 18.13%, 36.88% and 49.52% in RNAi‐treated females, respectively. The results suggested that AlinOBP4 might play dual‐roles in the identification of plant volatiles and sex pheromones. It was suspected that AlinOBP4 may have different functions in odor perception between male and female .
doi10.1111/1744-7917.12365
pages789-797
date2017-10