schliessen

Filtern

 

Bibliotheken

Liposome‐Encapsulated Hemoglobin Improves Tumor Oxygenation as Detected by Near‐Infrared Spectroscopy in Colon Carcinoma in Mice

Liposome‐encapsulated hemoglobin (LEH) with high (‐LEH, PO = 10 mm Hg) or low O affinity (‐LEH, PO = 40 mm Hg) may improve O delivery to sensitize tumor tissues for radiotherapy. A total of 10 mL/kg of ‐LEH, ‐LEH, red blood cells (RBCs), or saline was infused in mice transplanted with murine colon c... Full description

Journal Title: Artificial Organs April 2017, Vol.41(4), pp.327-335
Main Author: Kawaguchi, Fumio
Other Authors: Kawaguchi, Akira T. , Murayama, Chieko , Kamijo, Akemi , Haida, Munetaka
Format: Electronic Article Electronic Article
Language:
Subjects:
ID: ISSN: 0160-564X ; E-ISSN: 1525-1594 ; DOI: 10.1111/aor.12825
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: wj10.1111/aor.12825
title: Liposome‐Encapsulated Hemoglobin Improves Tumor Oxygenation as Detected by Near‐Infrared Spectroscopy in Colon Carcinoma in Mice
format: Article
creator:
  • Kawaguchi, Fumio
  • Kawaguchi, Akira T.
  • Murayama, Chieko
  • Kamijo, Akemi
  • Haida, Munetaka
subjects:
  • Tumor Hypoxia
  • —Hypoxic Fraction
  • —Near‐Infrared Spectroscopy
  • —Artificial Oxygen Carrier
  • —Liposome‐Encapsulated Hemoglobin
  • —Oxygen Affinity
  • —Radiosensitization
ispartof: Artificial Organs, April 2017, Vol.41(4), pp.327-335
description: Liposome‐encapsulated hemoglobin (LEH) with high (‐LEH, PO = 10 mm Hg) or low O affinity (‐LEH, PO = 40 mm Hg) may improve O delivery to sensitize tumor tissues for radiotherapy. A total of 10 mL/kg of ‐LEH, ‐LEH, red blood cells (RBCs), or saline was infused in mice transplanted with murine colon carcinoma with near‐infrared spectroscopy (NIRS) detectors set at the tumor (right leg) and intact muscle (left leg). NIRS recorded changes in the amount of oxyhemoglobin (oxyHb), deoxyhemoglobin (deoxyHb), and their sum (tHb) with the animals spontaneously breathing room air (10 min), pure O (5 min), and then back to room air. The tumor was finally excised for histological examination. In mice treated with ‐LEH, tHb significantly increased compared to mice receiving other solutions. The magnitude was significantly attenuated in the tumor compared to the intact muscle under room air. Reciprocal changes in oxyHb and deoxyHb between intact muscle and tumor in response to infused solutions allowed assumption of average tissue PO between 30 and 40 mm Hg in muscle and at around 10 mm Hg in tumor. While O respiration increased oxyHb and decreased deoxyHb both in muscle and tumor, their sum or tHb consistently decreased in muscle and increased in tumor regardless of preceding infusion. Such responses were totally reversed when mice were placed under hypoxia (10% O), suggesting that a lack of physiological circulatory regulation in tumor may account for heavier immunohistochemical staining for human hemoglobin in tumors of mice treated with ‐LEH than with ‐LEH. The results suggest that ‐LEH may cause significant tumor oxygenation compared to RBC, ‐LEH, or saline probably due to its nanometer size (vs. RBC) and high O affinity (vs. ‐LEH) without increasing O content in the intact tissue (vs. O respiration) probably due to a lack of physiological circulatory regulation.
language:
source:
identifier: ISSN: 0160-564X ; E-ISSN: 1525-1594 ; DOI: 10.1111/aor.12825
fulltext: fulltext
issn:
  • 0160-564X
  • 0160564X
  • 1525-1594
  • 15251594
url: Link


@attributes
ID558740141
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.1111/aor.12825
sourceidwj
recordidTN_wj10.1111/aor.12825
sourcesystemPC
pqid1886173282
galeid489072860
display
typearticle
titleLiposome‐Encapsulated Hemoglobin Improves Tumor Oxygenation as Detected by Near‐Infrared Spectroscopy in Colon Carcinoma in Mice
creatorKawaguchi, Fumio ; Kawaguchi, Akira T. ; Murayama, Chieko ; Kamijo, Akemi ; Haida, Munetaka
ispartofArtificial Organs, April 2017, Vol.41(4), pp.327-335
identifier
subjectTumor Hypoxia ; —Hypoxic Fraction ; —Near‐Infrared Spectroscopy ; —Artificial Oxygen Carrier ; —Liposome‐Encapsulated Hemoglobin ; —Oxygen Affinity ; —Radiosensitization
descriptionLiposome‐encapsulated hemoglobin (LEH) with high (‐LEH, PO = 10 mm Hg) or low O affinity (‐LEH, PO = 40 mm Hg) may improve O delivery to sensitize tumor tissues for radiotherapy. A total of 10 mL/kg of ‐LEH, ‐LEH, red blood cells (RBCs), or saline was infused in mice transplanted with murine colon carcinoma with near‐infrared spectroscopy (NIRS) detectors set at the tumor (right leg) and intact muscle (left leg). NIRS recorded changes in the amount of oxyhemoglobin (oxyHb), deoxyhemoglobin (deoxyHb), and their sum (tHb) with the animals spontaneously breathing room air (10 min), pure O (5 min), and then back to room air. The tumor was finally excised for histological examination. In mice treated with ‐LEH, tHb significantly increased compared to mice receiving other solutions. The magnitude was significantly attenuated in the tumor compared to the intact muscle under room air. Reciprocal changes in oxyHb and deoxyHb between intact muscle and tumor in response to infused solutions allowed assumption of average tissue PO between 30 and 40 mm Hg in muscle and at around 10 mm Hg in tumor. While O respiration increased oxyHb and decreased deoxyHb both in muscle and tumor, their sum or tHb consistently decreased in muscle and increased in tumor regardless of preceding infusion. Such responses were totally reversed when mice were placed under hypoxia (10% O), suggesting that a lack of physiological circulatory regulation in tumor may account for heavier immunohistochemical staining for human hemoglobin in tumors of mice treated with ‐LEH than with ‐LEH. The results suggest that ‐LEH may cause significant tumor oxygenation compared to RBC, ‐LEH, or saline probably due to its nanometer size (vs. RBC) and high O affinity (vs. ‐LEH) without increasing O content in the intact tissue (vs. O respiration) probably due to a lack of physiological circulatory regulation.
source
version8
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Kawaguchi, Fumio
1Kawaguchi, Akira T.
2Murayama, Chieko
3Kamijo, Akemi
4Haida, Munetaka
titleLiposome‐Encapsulated Hemoglobin Improves Tumor Oxygenation as Detected by Near‐Infrared Spectroscopy in Colon Carcinoma in Mice
descriptionLiposome‐encapsulated hemoglobin (LEH) with high (‐LEH, PO = 10 mm Hg) or low O affinity (‐LEH, PO = 40 mm Hg) may improve O delivery to sensitize tumor tissues for radiotherapy. A total of 10 mL/kg of ‐LEH, ‐LEH, red blood cells (RBCs), or saline was infused in mice transplanted with murine colon carcinoma with near‐infrared spectroscopy (NIRS) detectors set at the tumor (right leg) and intact muscle (left leg). NIRS recorded changes in the amount of oxyhemoglobin (oxyHb), deoxyhemoglobin (deoxyHb), and their sum (tHb) with the animals spontaneously breathing room air (10 min), pure O (5 min), and then back to room air. The tumor was finally excised for histological examination. In mice treated with ‐LEH, tHb significantly increased compared to mice receiving other solutions. The magnitude was significantly attenuated in the tumor compared to the intact muscle under room air. Reciprocal changes in oxyHb and deoxyHb between intact muscle and tumor in response to infused solutions allowed assumption of average tissue PO between 30 and 40 mm Hg in muscle and at around 10 mm Hg in tumor. While O respiration increased oxyHb and decreased deoxyHb both in muscle and tumor, their sum or tHb consistently decreased in muscle and increased in tumor regardless of preceding infusion. Such responses were totally reversed when mice were placed under hypoxia (10% O), suggesting that a lack of physiological circulatory regulation in tumor may account for heavier immunohistochemical staining for human hemoglobin in tumors of mice treated with ‐LEH than with ‐LEH. The results suggest that ‐LEH may cause significant tumor oxygenation compared to RBC, ‐LEH, or saline probably due to its nanometer size (vs. RBC) and high O affinity (vs. ‐LEH) without increasing O content in the intact tissue (vs. O respiration) probably due to a lack of physiological circulatory regulation.
subject
0Tumor Hypoxia
1—Hypoxic Fraction
2—Near‐Infrared Spectroscopy
3—Artificial Oxygen Carrier
4—Liposome‐Encapsulated Hemoglobin
5—Oxygen Affinity
6—Radiosensitization
general
010.1111/aor.12825
1Wiley Online Library
sourceidwj
recordidwj10.1111/aor.12825
issn
00160-564X
10160564X
21525-1594
315251594
rsrctypearticle
creationdate2017
addtitleArtificial Organs
searchscope
0wj
1wiley
scope
0wj
1wiley
lsr30VSR-Enriched:[galeid, pages, pqid]
sort
titleLiposome‐Encapsulated Hemoglobin Improves Tumor Oxygenation as Detected by Near‐Infrared Spectroscopy in Colon Carcinoma in Mice
authorKawaguchi, Fumio ; Kawaguchi, Akira T. ; Murayama, Chieko ; Kamijo, Akemi ; Haida, Munetaka
creationdate20170400
facets
frbrgroupid6202765463125123249
frbrtype5
newrecords20170503
creationdate2017
topic
0Tumor Hypoxia
1—Hypoxic Fraction
2—Near‐Infrared Spectroscopy
3—Artificial Oxygen Carrier
4—Liposome‐Encapsulated Hemoglobin
5—Oxygen Affinity
6—Radiosensitization
collectionWiley Online Library
prefilterarticles
rsrctypearticles
creatorcontrib
0Kawaguchi, Fumio
1Kawaguchi, Akira T.
2Murayama, Chieko
3Kamijo, Akemi
4Haida, Munetaka
jtitleArtificial Organs
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Kawaguchi
1Murayama
2Kamijo
3Haida
aufirst
0Fumio
1Akira T.
2Chieko
3Akemi
4Munetaka
au
0Kawaguchi, Fumio
1Kawaguchi, Akira T.
2Murayama, Chieko
3Kamijo, Akemi
4Haida, Munetaka
atitleLiposome‐Encapsulated Hemoglobin Improves Tumor Oxygenation as Detected by Near‐Infrared Spectroscopy in Colon Carcinoma in Mice
jtitleArtificial Organs
risdate201704
volume41
issue4
spage327
epage335
issn0160-564X
eissn1525-1594
genrearticle
ristypeJOUR
abstractLiposome‐encapsulated hemoglobin (LEH) with high (‐LEH, PO = 10 mm Hg) or low O affinity (‐LEH, PO = 40 mm Hg) may improve O delivery to sensitize tumor tissues for radiotherapy. A total of 10 mL/kg of ‐LEH, ‐LEH, red blood cells (RBCs), or saline was infused in mice transplanted with murine colon carcinoma with near‐infrared spectroscopy (NIRS) detectors set at the tumor (right leg) and intact muscle (left leg). NIRS recorded changes in the amount of oxyhemoglobin (oxyHb), deoxyhemoglobin (deoxyHb), and their sum (tHb) with the animals spontaneously breathing room air (10 min), pure O (5 min), and then back to room air. The tumor was finally excised for histological examination. In mice treated with ‐LEH, tHb significantly increased compared to mice receiving other solutions. The magnitude was significantly attenuated in the tumor compared to the intact muscle under room air. Reciprocal changes in oxyHb and deoxyHb between intact muscle and tumor in response to infused solutions allowed assumption of average tissue PO between 30 and 40 mm Hg in muscle and at around 10 mm Hg in tumor. While O respiration increased oxyHb and decreased deoxyHb both in muscle and tumor, their sum or tHb consistently decreased in muscle and increased in tumor regardless of preceding infusion. Such responses were totally reversed when mice were placed under hypoxia (10% O), suggesting that a lack of physiological circulatory regulation in tumor may account for heavier immunohistochemical staining for human hemoglobin in tumors of mice treated with ‐LEH than with ‐LEH. The results suggest that ‐LEH may cause significant tumor oxygenation compared to RBC, ‐LEH, or saline probably due to its nanometer size (vs. RBC) and high O affinity (vs. ‐LEH) without increasing O content in the intact tissue (vs. O respiration) probably due to a lack of physiological circulatory regulation.
doi10.1111/aor.12825
pages327-335
date2017-04