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Characterization of Helicobacter pylori VacA‐containing vacuoles (VCVs), VacA intracellular trafficking and interference with calcium signalling in T lymphocytes

The human pathogen colonizes half of the global population. Residing at the stomach epithelium, it contributes to the development of diseases such as gastritis, duodenal and gastric ulcers, and gastric cancer. A major factor is the secreted vacuolating toxin , which forms anion‐selective channels in... Full description

Journal Title: Cellular Microbiology December 2015, Vol.17(12), pp.1811-1832
Main Author: Kern, Beate
Other Authors: Jain, Utkarsh , Utsch, Ciara , Otto, Andreas , Busch, Benjamin , Jiménez‐Soto, Luisa , Becher, Dörte , Haas, Rainer
Format: Electronic Article Electronic Article
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ID: ISSN: 1462-5814 ; E-ISSN: 1462-5822 ; DOI: 10.1111/cmi.12474
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recordid: wj10.1111/cmi.12474
title: Characterization of Helicobacter pylori VacA‐containing vacuoles (VCVs), VacA intracellular trafficking and interference with calcium signalling in T lymphocytes
format: Article
creator:
  • Kern, Beate
  • Jain, Utkarsh
  • Utsch, Ciara
  • Otto, Andreas
  • Busch, Benjamin
  • Jiménez‐Soto, Luisa
  • Becher, Dörte
  • Haas, Rainer
subjects:
  • T Cells
  • Helicobacter Pylori
ispartof: Cellular Microbiology, December 2015, Vol.17(12), pp.1811-1832
description: The human pathogen colonizes half of the global population. Residing at the stomach epithelium, it contributes to the development of diseases such as gastritis, duodenal and gastric ulcers, and gastric cancer. A major factor is the secreted vacuolating toxin , which forms anion‐selective channels in the endosome membrane that cause the compartment to swell, but the composition and purpose of the resulting ‐containing vacuoles () are still unknown. exerts influence on the host immune response in various ways, including inhibition of T‐cell activation and proliferation and suppression of the host immune response. In this study, for the first time the composition of from cells was comprehensively analysed to investigate function. were successfully isolated via immunomagnetic separation, and the purified vacuoles were analysed by mass spectrometry. We detected a set of 122 ‐specific proteins implicated among others in immune response, cell death and cellular signalling processes, all of which is known to influence. One of the individual proteins studied further was romal nteraction olecule (), a calcium sensor residing in the endoplasmic reticulum () that is important in store‐operated calcium entry. Live cell imaging microscopy data demonstrated colocalization of with STIM1 in the and indicated that may interfere with the movement of STIM1 towards the plasma membrane‐localized alcium elease ctivated alcium channel protein in response to store depletion. Furthermore, inhibited the increase of cytosolic‐free in the urkat 6‐1 ‐cell line and human 4 T cells. The presence of in the and its trafficking to the Golgi apparatus was confirmed in HeLa cells, identifying these two cellular compartments as novel target structures.
language:
source:
identifier: ISSN: 1462-5814 ; E-ISSN: 1462-5822 ; DOI: 10.1111/cmi.12474
fulltext: fulltext
issn:
  • 1462-5814
  • 14625814
  • 1462-5822
  • 14625822
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titleCharacterization of Helicobacter pylori VacA‐containing vacuoles (VCVs), VacA intracellular trafficking and interference with calcium signalling in T lymphocytes
creatorKern, Beate ; Jain, Utkarsh ; Utsch, Ciara ; Otto, Andreas ; Busch, Benjamin ; Jiménez‐Soto, Luisa ; Becher, Dörte ; Haas, Rainer
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descriptionThe human pathogen colonizes half of the global population. Residing at the stomach epithelium, it contributes to the development of diseases such as gastritis, duodenal and gastric ulcers, and gastric cancer. A major factor is the secreted vacuolating toxin , which forms anion‐selective channels in the endosome membrane that cause the compartment to swell, but the composition and purpose of the resulting ‐containing vacuoles () are still unknown. exerts influence on the host immune response in various ways, including inhibition of T‐cell activation and proliferation and suppression of the host immune response. In this study, for the first time the composition of from cells was comprehensively analysed to investigate function. were successfully isolated via immunomagnetic separation, and the purified vacuoles were analysed by mass spectrometry. We detected a set of 122 ‐specific proteins implicated among others in immune response, cell death and cellular signalling processes, all of which is known to influence. One of the individual proteins studied further was romal nteraction olecule (), a calcium sensor residing in the endoplasmic reticulum () that is important in store‐operated calcium entry. Live cell imaging microscopy data demonstrated colocalization of with STIM1 in the and indicated that may interfere with the movement of STIM1 towards the plasma membrane‐localized alcium elease ctivated alcium channel protein in response to store depletion. Furthermore, inhibited the increase of cytosolic‐free in the urkat 6‐1 ‐cell line and human 4 T cells. The presence of in the and its trafficking to the Golgi apparatus was confirmed in HeLa cells, identifying these two cellular compartments as novel target structures.
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titleCharacterization of Helicobacter pylori VacA‐containing vacuoles (VCVs), VacA intracellular trafficking and interference with calcium signalling in T lymphocytes
descriptionThe human pathogen colonizes half of the global population. Residing at the stomach epithelium, it contributes to the development of diseases such as gastritis, duodenal and gastric ulcers, and gastric cancer. A major factor is the secreted vacuolating toxin , which forms anion‐selective channels in the endosome membrane that cause the compartment to swell, but the composition and purpose of the resulting ‐containing vacuoles () are still unknown. exerts influence on the host immune response in various ways, including inhibition of T‐cell activation and proliferation and suppression of the host immune response. In this study, for the first time the composition of from cells was comprehensively analysed to investigate function. were successfully isolated via immunomagnetic separation, and the purified vacuoles were analysed by mass spectrometry. We detected a set of 122 ‐specific proteins implicated among others in immune response, cell death and cellular signalling processes, all of which is known to influence. One of the individual proteins studied further was romal nteraction olecule (), a calcium sensor residing in the endoplasmic reticulum () that is important in store‐operated calcium entry. Live cell imaging microscopy data demonstrated colocalization of with STIM1 in the and indicated that may interfere with the movement of STIM1 towards the plasma membrane‐localized alcium elease ctivated alcium channel protein in response to store depletion. Furthermore, inhibited the increase of cytosolic‐free in the urkat 6‐1 ‐cell line and human 4 T cells. The presence of in the and its trafficking to the Golgi apparatus was confirmed in HeLa cells, identifying these two cellular compartments as novel target structures.
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abstractThe human pathogen colonizes half of the global population. Residing at the stomach epithelium, it contributes to the development of diseases such as gastritis, duodenal and gastric ulcers, and gastric cancer. A major factor is the secreted vacuolating toxin , which forms anion‐selective channels in the endosome membrane that cause the compartment to swell, but the composition and purpose of the resulting ‐containing vacuoles () are still unknown. exerts influence on the host immune response in various ways, including inhibition of T‐cell activation and proliferation and suppression of the host immune response. In this study, for the first time the composition of from cells was comprehensively analysed to investigate function. were successfully isolated via immunomagnetic separation, and the purified vacuoles were analysed by mass spectrometry. We detected a set of 122 ‐specific proteins implicated among others in immune response, cell death and cellular signalling processes, all of which is known to influence. One of the individual proteins studied further was romal nteraction olecule (), a calcium sensor residing in the endoplasmic reticulum () that is important in store‐operated calcium entry. Live cell imaging microscopy data demonstrated colocalization of with STIM1 in the and indicated that may interfere with the movement of STIM1 towards the plasma membrane‐localized alcium elease ctivated alcium channel protein in response to store depletion. Furthermore, inhibited the increase of cytosolic‐free in the urkat 6‐1 ‐cell line and human 4 T cells. The presence of in the and its trafficking to the Golgi apparatus was confirmed in HeLa cells, identifying these two cellular compartments as novel target structures.
doi10.1111/cmi.12474
pages1811-1832
date2015-12