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Enhanced gastric cancer growth potential of mesenchymal stem cells derived from gastric cancer tissues educated by CD4+ T cells

To purchase or authenticate to the full-text of this article, please visit this link: http://onlinelibrary.wiley.com/doi/10.1111/cpr.12399/abstract Byline: Rongman Xu, Xiangdong Zhao, Yuanyuan Zhao, Bin Chen, Li Sun, Changgen Xu, Bo Shen, Mei Wang, Wenrong Xu,Wei Zhu Abstract Objectives Gastric canc... Full description

Journal Title: Cell Proliferation April 2018, Vol.51(2), pp.n/a-n/a
Main Author: Xu, Rongman
Other Authors: Zhao, Xiangdong , Zhao, Yuanyuan , Chen, Bin , Sun, Li , Xu, Changgen , Shen, Bo , Wang, Mei , Xu, Wenrong , Zhu, Wei
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ID: ISSN: 0960-7722 ; E-ISSN: 1365-2184 ; DOI: 10.1111/cpr.12399
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recordid: wj10.1111/cpr.12399
title: Enhanced gastric cancer growth potential of mesenchymal stem cells derived from gastric cancer tissues educated by CD4+ T cells
format: Article
creator:
  • Xu, Rongman
  • Zhao, Xiangdong
  • Zhao, Yuanyuan
  • Chen, Bin
  • Sun, Li
  • Xu, Changgen
  • Shen, Bo
  • Wang, Mei
  • Xu, Wenrong
  • Zhu, Wei
subjects:
  • Cancer -- Analysis
  • T Cells -- Growth
  • T Cells -- Analysis
  • Stem Cell Transplantation -- Analysis
  • Stem Cells -- Analysis
  • Stomach Cancer -- Analysis
ispartof: Cell Proliferation, April 2018, Vol.51(2), pp.n/a-n/a
description: To purchase or authenticate to the full-text of this article, please visit this link: http://onlinelibrary.wiley.com/doi/10.1111/cpr.12399/abstract Byline: Rongman Xu, Xiangdong Zhao, Yuanyuan Zhao, Bin Chen, Li Sun, Changgen Xu, Bo Shen, Mei Wang, Wenrong Xu,Wei Zhu Abstract Objectives Gastric cancer mesenchymal stem cells (GC-MSCs) can promote the development of tumour growth. The tumour-promoting role of tumour-associated MSCs and T cells has been demonstrated. T cells as the major immune cells may influence and induce a pro-tumour phenotype in MSCs. This study focused on whether CD4.sub.+ T cells can affect GC-MSCs to promote gastric cancer growth. Materials and methods CD4.sub.+ T cells upregulation of programmed death ligand 1 (PD-L1) expression in GC-MSCs through the phosphorylated signal transducer and activator of transcription (p-STAT3) signalling pathway was confirmed by immunofluorescence, western blotting and RT-PCR. Migration of GC cells was detected by Transwell migration assay, and apoptosis of GC cells was measured by flow cytometry using annexin V/propidium iodide double staining. CD4.sub.+ T cell-primed GC-MSCs promoted GC growth in a subcutaneously transplanted tumour model in BALB/c nu/nu mice. Results Gastric cancer mesenchymal stem cells stimulated by activated CD4.sub.+ T cells promoted migration of GC cells and enhanced GC growth potential in BALB/c nu/nu xenografts. PD-L1 upregulation of GC-MSCs stimulated by CD4.sub.+ T cells was mediated through the p-STAT3 signalling pathway. CD4.sub.+ T cells-primed GC-MSCs have greater GC volume and growth rate-promoting role than GC-MSCs, with cancer cell-intrinsic PD-1/mammalian target of rapamycin (mTOR) signalling activation. Conclusions This study showed that GC-MSCs are plastic. The immunophenotype of GC-MSCs stimulated by CD4.sub.+ T cells has major changes that may influence tumour cell growth. This research was based on the interaction between tumour cells, MSCs and immune cells, providing a new understanding of the development and immunotherapy of GC. Article Note: Funding information This study was supported by the National Science Foundation of China (Grant number: 81472334) and Jiangsu province social development key research and development plan (Grant number: BE2017694). Rongman Xu and Xiangdong Zhao contributed equally to this work.
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source:
identifier: ISSN: 0960-7722 ; E-ISSN: 1365-2184 ; DOI: 10.1111/cpr.12399
fulltext: fulltext
issn:
  • 0960-7722
  • 09607722
  • 1365-2184
  • 13652184
url: Link


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titleEnhanced gastric cancer growth potential of mesenchymal stem cells derived from gastric cancer tissues educated by CD4+ T cells
creatorXu, Rongman ; Zhao, Xiangdong ; Zhao, Yuanyuan ; Chen, Bin ; Sun, Li ; Xu, Changgen ; Shen, Bo ; Wang, Mei ; Xu, Wenrong ; Zhu, Wei
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descriptionTo purchase or authenticate to the full-text of this article, please visit this link: http://onlinelibrary.wiley.com/doi/10.1111/cpr.12399/abstract Byline: Rongman Xu, Xiangdong Zhao, Yuanyuan Zhao, Bin Chen, Li Sun, Changgen Xu, Bo Shen, Mei Wang, Wenrong Xu,Wei Zhu Abstract Objectives Gastric cancer mesenchymal stem cells (GC-MSCs) can promote the development of tumour growth. The tumour-promoting role of tumour-associated MSCs and T cells has been demonstrated. T cells as the major immune cells may influence and induce a pro-tumour phenotype in MSCs. This study focused on whether CD4.sub.+ T cells can affect GC-MSCs to promote gastric cancer growth. Materials and methods CD4.sub.+ T cells upregulation of programmed death ligand 1 (PD-L1) expression in GC-MSCs through the phosphorylated signal transducer and activator of transcription (p-STAT3) signalling pathway was confirmed by immunofluorescence, western blotting and RT-PCR. Migration of GC cells was detected by Transwell migration assay, and apoptosis of GC cells was measured by flow cytometry using annexin V/propidium iodide double staining. CD4.sub.+ T cell-primed GC-MSCs promoted GC growth in a subcutaneously transplanted tumour model in BALB/c nu/nu mice. Results Gastric cancer mesenchymal stem cells stimulated by activated CD4.sub.+ T cells promoted migration of GC cells and enhanced GC growth potential in BALB/c nu/nu xenografts. PD-L1 upregulation of GC-MSCs stimulated by CD4.sub.+ T cells was mediated through the p-STAT3 signalling pathway. CD4.sub.+ T cells-primed GC-MSCs have greater GC volume and growth rate-promoting role than GC-MSCs, with cancer cell-intrinsic PD-1/mammalian target of rapamycin (mTOR) signalling activation. Conclusions This study showed that GC-MSCs are plastic. The immunophenotype of GC-MSCs stimulated by CD4.sub.+ T cells has major changes that may influence tumour cell growth. This research was based on the interaction between tumour cells, MSCs and immune cells, providing a new understanding of the development and immunotherapy of GC. Article Note: Funding information This study was supported by the National Science Foundation of China (Grant number: 81472334) and Jiangsu province social development key research and development plan (Grant number: BE2017694). Rongman Xu and Xiangdong Zhao contributed equally to this work.
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