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The CB1 cannabinoid receptor C‐terminus regulates receptor desensitization in autaptic hippocampal neurones

The cannabinoid CB receptor is the chief mediator of the CNS effects of cannabinoids. In cell culture model systems, CB receptors both desensitize and internalize on activation. Previous work suggests that the extreme carboxy‐terminus of this receptor regulates internalization via phosphorylation of... Full description

Journal Title: British Journal of Pharmacology April 2012, Vol.165(8), pp.2652-2659
Main Author: Straiker, Alex
Other Authors: Wager‐Miller, Jim , Mackie, Ken
Format: Electronic Article Electronic Article
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ID: ISSN: 0007-1188 ; E-ISSN: 1476-5381 ; DOI: 10.1111/j.1476-5381.2011.01743.x
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recordid: wj10.1111/j.1476-5381.2011.01743.x
title: The CB1 cannabinoid receptor C‐terminus regulates receptor desensitization in autaptic hippocampal neurones
format: Article
creator:
  • Straiker, Alex
  • Wager‐Miller, Jim
  • Mackie, Ken
subjects:
  • Endocannabinoid
  • Dse
  • 2‐Ag
  • Retrograde Transmission
ispartof: British Journal of Pharmacology, April 2012, Vol.165(8), pp.2652-2659
description: The cannabinoid CB receptor is the chief mediator of the CNS effects of cannabinoids. In cell culture model systems, CB receptors both desensitize and internalize on activation. Previous work suggests that the extreme carboxy‐terminus of this receptor regulates internalization via phosphorylation of residues clustered within this region. Mutational analysis of the carboxy‐terminus of CB receptors has demonstrated that the last six serine/threonine residues are necessary for agonist‐induced internalization. However, the structural determinants of CB receptor internalization are also dependent on the local cellular environment. The importance of cell context on CB receptor function calls for an investigation of the functional roles of these residues in neurones. To determine the structural requirements of CB internalization in neurones, we evaluated the signalling properties of carboxy‐terminal mutated CB receptors expressed in cultured autaptic hippocampal neurones, using electrophysiological methods. CB receptors transfected into CB knockout neurones signalled and desensitized as did wild‐type neurones, allowing us to test specific CB receptor mutations. Deletion of the last 13 residues yielded a CB receptor that inhibited excitatory postsynaptic currents but did not desensitize. Furthermore, mutation of the final six serine and threonine residues to alanines resulted in a non‐desensitizing receptor. In contrast, CB receptors lacking residues 419–460, leaving the last 14 residues intact, did desensitize. The distal thirteen residues of CB receptors are crucial for their desensitization in cultured neurones. Furthermore, this desensitization is likely to follow phosphorylation of serines and threonines within this region. This article is part of a themed section on Cannabinoids in Biology and Medicine. To view the other articles in this section visit . To view Part I of Cannabinoids in Biology and Medicine visit
language:
source:
identifier: ISSN: 0007-1188 ; E-ISSN: 1476-5381 ; DOI: 10.1111/j.1476-5381.2011.01743.x
fulltext: fulltext
issn:
  • 0007-1188
  • 00071188
  • 1476-5381
  • 14765381
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titleThe CB1 cannabinoid receptor C‐terminus regulates receptor desensitization in autaptic hippocampal neurones
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descriptionThe cannabinoid CB receptor is the chief mediator of the CNS effects of cannabinoids. In cell culture model systems, CB receptors both desensitize and internalize on activation. Previous work suggests that the extreme carboxy‐terminus of this receptor regulates internalization via phosphorylation of residues clustered within this region. Mutational analysis of the carboxy‐terminus of CB receptors has demonstrated that the last six serine/threonine residues are necessary for agonist‐induced internalization. However, the structural determinants of CB receptor internalization are also dependent on the local cellular environment. The importance of cell context on CB receptor function calls for an investigation of the functional roles of these residues in neurones. To determine the structural requirements of CB internalization in neurones, we evaluated the signalling properties of carboxy‐terminal mutated CB receptors expressed in cultured autaptic hippocampal neurones, using electrophysiological methods. CB receptors transfected into CB knockout neurones signalled and desensitized as did wild‐type neurones, allowing us to test specific CB receptor mutations. Deletion of the last 13 residues yielded a CB receptor that inhibited excitatory postsynaptic currents but did not desensitize. Furthermore, mutation of the final six serine and threonine residues to alanines resulted in a non‐desensitizing receptor. In contrast, CB receptors lacking residues 419–460, leaving the last 14 residues intact, did desensitize. The distal thirteen residues of CB receptors are crucial for their desensitization in cultured neurones. Furthermore, this desensitization is likely to follow phosphorylation of serines and threonines within this region. This article is part of a themed section on Cannabinoids in Biology and Medicine. To view the other articles in this section visit . To view Part I of Cannabinoids in Biology and Medicine visit
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abstractThe cannabinoid CB receptor is the chief mediator of the CNS effects of cannabinoids. In cell culture model systems, CB receptors both desensitize and internalize on activation. Previous work suggests that the extreme carboxy‐terminus of this receptor regulates internalization via phosphorylation of residues clustered within this region. Mutational analysis of the carboxy‐terminus of CB receptors has demonstrated that the last six serine/threonine residues are necessary for agonist‐induced internalization. However, the structural determinants of CB receptor internalization are also dependent on the local cellular environment. The importance of cell context on CB receptor function calls for an investigation of the functional roles of these residues in neurones. To determine the structural requirements of CB internalization in neurones, we evaluated the signalling properties of carboxy‐terminal mutated CB receptors expressed in cultured autaptic hippocampal neurones, using electrophysiological methods. CB receptors transfected into CB knockout neurones signalled and desensitized as did wild‐type neurones, allowing us to test specific CB receptor mutations. Deletion of the last 13 residues yielded a CB receptor that inhibited excitatory postsynaptic currents but did not desensitize. Furthermore, mutation of the final six serine and threonine residues to alanines resulted in a non‐desensitizing receptor. In contrast, CB receptors lacking residues 419–460, leaving the last 14 residues intact, did desensitize. The distal thirteen residues of CB receptors are crucial for their desensitization in cultured neurones. Furthermore, this desensitization is likely to follow phosphorylation of serines and threonines within this region. This article is part of a themed section on Cannabinoids in Biology and Medicine. To view the other articles in this section visit . To view Part I of Cannabinoids in Biology and Medicine visit
copOxford, UK
pubBlackwell Publishing Ltd
doi10.1111/j.1476-5381.2011.01743.x
pages2652-2659
date2012-04