schliessen

Filtern

 

Bibliotheken

Ovarian Stimulation Reduces IL‐6 Release from Mouse and Human Pre‐Implantation Embryos

In our previous study, ovarian stimulation retarded post-implantation development and caused 92 genes, including interleukin-6 (IL-6), to be differentially expressed in mouse blastocysts. None of them had been tested at protein level, In this study, we confirm the level change of IL-6 mRNA in mouse... Full description

Journal Title: American Journal of Reproductive Immunology September 2012, Vol.68(3), pp.199-204
Main Author: Yu, Chunhua
Other Authors: Wang, Li , Li, Jianhua , Guo, Chun , Guo, Xinyu , Zhang, Xinyan , Xu, Ye , Yao, Yuanqing
Format: Electronic Article Electronic Article
Language:
Subjects:
ID: ISSN: 1046-7408 ; E-ISSN: 1600-0897 ; DOI: 10.1111/j.1600-0897.2012.01142.x
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: wj10.1111/j.1600-0897.2012.01142.x
title: Ovarian Stimulation Reduces IL‐6 Release from Mouse and Human Pre‐Implantation Embryos
format: Article
creator:
  • Yu, Chunhua
  • Wang, Li
  • Li, Jianhua
  • Guo, Chun
  • Guo, Xinyu
  • Zhang, Xinyan
  • Xu, Ye
  • Yao, Yuanqing
subjects:
  • Embryos
  • Il ‐6
  • Pre‐Implantation
  • Ovarian Stimulation
ispartof: American Journal of Reproductive Immunology, September 2012, Vol.68(3), pp.199-204
description: In our previous study, ovarian stimulation retarded post-implantation development and caused 92 genes, including interleukin-6 (IL-6), to be differentially expressed in mouse blastocysts. None of them had been tested at protein level, In this study, we confirm the level change of IL-6 mRNA in mouse blastocysts and IL-6 protein release to the culture medium from mouse and human pre-implantation embryos. IL-6 mRNA were measured by PCR and IL-6 release to the culture medium were measured by ELISA. Superovulation caused a 1.95-fold reduction in IL-6 mRNA in mouse blastocysts and reduced IL-6 secretion into the culture medium in mouse zygotes which were developed to the blastocyst stage (6.397 plus or minus 0.635 versus 4.799 plus or minus 0.312 pg/mL). Ovarian stimulation also significantly reduced IL-6 release into the culture medium in human zygotes which were cultured for 2 days (4.713 plus or minus 0.945 versus 4.543 plus or minus 0.796 pg/mL). Our findings indicate that IL-6 might serve as a candidate marker for evaluating embryo developmental competence.
language:
source:
identifier: ISSN: 1046-7408 ; E-ISSN: 1600-0897 ; DOI: 10.1111/j.1600-0897.2012.01142.x
fulltext: fulltext
issn:
  • 1046-7408
  • 10467408
  • 1600-0897
  • 16000897
url: Link


@attributes
ID450691753
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.1111/j.1600-0897.2012.01142.x
sourceidwj
recordidTN_wj10.1111/j.1600-0897.2012.01142.x
sourcesystemOther
pqid1038606591
display
typearticle
titleOvarian Stimulation Reduces IL‐6 Release from Mouse and Human Pre‐Implantation Embryos
creatorYu, Chunhua ; Wang, Li ; Li, Jianhua ; Guo, Chun ; Guo, Xinyu ; Zhang, Xinyan ; Xu, Ye ; Yao, Yuanqing
ispartofAmerican Journal of Reproductive Immunology, September 2012, Vol.68(3), pp.199-204
identifier
subjectEmbryos ; Il ‐6 ; Pre‐Implantation ; Ovarian Stimulation
source
descriptionIn our previous study, ovarian stimulation retarded post-implantation development and caused 92 genes, including interleukin-6 (IL-6), to be differentially expressed in mouse blastocysts. None of them had been tested at protein level, In this study, we confirm the level change of IL-6 mRNA in mouse blastocysts and IL-6 protein release to the culture medium from mouse and human pre-implantation embryos. IL-6 mRNA were measured by PCR and IL-6 release to the culture medium were measured by ELISA. Superovulation caused a 1.95-fold reduction in IL-6 mRNA in mouse blastocysts and reduced IL-6 secretion into the culture medium in mouse zygotes which were developed to the blastocyst stage (6.397 plus or minus 0.635 versus 4.799 plus or minus 0.312 pg/mL). Ovarian stimulation also significantly reduced IL-6 release into the culture medium in human zygotes which were cultured for 2 days (4.713 plus or minus 0.945 versus 4.543 plus or minus 0.796 pg/mL). Our findings indicate that IL-6 might serve as a candidate marker for evaluating embryo developmental competence.
version5
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Yu, Chunhua
1Wang, Li
2Li, Jianhua
3Guo, Chun
4Guo, Xinyu
5Zhang, Xinyan
6Xu, Ye
7Yao, Yuanqing
titleOvarian Stimulation Reduces IL‐6 Release from Mouse and Human Pre‐Implantation Embryos
subject
0Embryos
1Il ‐6
2Pre‐Implantation
3Ovarian Stimulation
general
010.1111/j.1600-0897.2012.01142.x
1Wiley Online Library
sourceidwj
recordidwj10.1111/j.1600-0897.2012.01142.x
issn
01046-7408
110467408
21600-0897
316000897
rsrctypearticle
creationdate2012
addtitle
0American Journal of Reproductive Immunology
1Am J Reprod Immunol
searchscope
0wj
1wiley
scope
0wj
1wiley
lsr30VSR-Enriched:[description, pages, pqid]
sort
titleOvarian Stimulation Reduces IL‐6 Release from Mouse and Human Pre‐Implantation Embryos
authorYu, Chunhua ; Wang, Li ; Li, Jianhua ; Guo, Chun ; Guo, Xinyu ; Zhang, Xinyan ; Xu, Ye ; Yao, Yuanqing
creationdate20120900
facets
frbrgroupid4844191735754082748
frbrtype5
creationdate2012
topic
0Embryos
1Il ‐6
2Pre‐Implantation
3Ovarian Stimulation
collectionWiley Online Library
prefilterarticles
rsrctypearticles
creatorcontrib
0Yu, Chunhua
1Wang, Li
2Li, Jianhua
3Guo, Chun
4Guo, Xinyu
5Zhang, Xinyan
6Xu, Ye
7Yao, Yuanqing
jtitleAmerican Journal of Reproductive Immunology
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Yu
1Wang
2Li
3Guo
4Zhang
5Xu
6Yao
aufirst
0Chunhua
1Li
2Jianhua
3Chun
4Xinyu
5Xinyan
6Ye
7Yuanqing
au
0Yu, Chunhua
1Wang, Li
2Li, Jianhua
3Guo, Chun
4Guo, Xinyu
5Zhang, Xinyan
6Xu, Ye
7Yao, Yuanqing
atitleOvarian Stimulation Reduces IL‐6 Release from Mouse and Human Pre‐Implantation Embryos
jtitleAmerican Journal of Reproductive Immunology
risdate201209
volume68
issue3
spage199
epage204
issn1046-7408
eissn1600-0897
genrearticle
ristypeJOUR
doi10.1111/j.1600-0897.2012.01142.x
pages199-204
date2012-09