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Simultaneous Spectrophotometric Determination of 2‐Thiouracil and 2‐Mercaptobenzimidazole in Animal Tissue Using Multivariate Calibration Methods: Concerns and Rapid Methods for Detection

Two multivariate calibration methods, partial least squares (PLS) and principal component regression (PCR), were applied to the spectrophotometric simultaneous determination of 2‐mercaptobenzimidazole (MB) and 2‐thiouracil (TU). A genetic algorithm (GA) using partial least squares was successfully u... Full description

Journal Title: Journal of Food Science March 2010, Vol.75(2), pp.C135-C139
Main Author: Beheshti, Abolghasem
Other Authors: Riahi, Siavash , Pourbasheer, Eslam , Ganjali, Mohammad Reza , Norouzi, Parviz
Format: Electronic Article Electronic Article
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ID: ISSN: 0022-1147 ; E-ISSN: 1750-3841 ; DOI: 10.1111/j.1750-3841.2009.01458.x
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recordid: wj10.1111/j.1750-3841.2009.01458.x
title: Simultaneous Spectrophotometric Determination of 2‐Thiouracil and 2‐Mercaptobenzimidazole in Animal Tissue Using Multivariate Calibration Methods: Concerns and Rapid Methods for Detection
format: Article
creator:
  • Beheshti, Abolghasem
  • Riahi, Siavash
  • Pourbasheer, Eslam
  • Ganjali, Mohammad Reza
  • Norouzi, Parviz
subjects:
  • Animal Tissue
  • Chemometrics
  • Ga‐Pls
  • Spectrophotometric
  • 2‐Mercaptobenzimidazole
  • 2‐Thiouracil
ispartof: Journal of Food Science, March 2010, Vol.75(2), pp.C135-C139
description: Two multivariate calibration methods, partial least squares (PLS) and principal component regression (PCR), were applied to the spectrophotometric simultaneous determination of 2‐mercaptobenzimidazole (MB) and 2‐thiouracil (TU). A genetic algorithm (GA) using partial least squares was successfully utilized as a variable selection method. The concentration model was based on the absorption spectra in the range of 200 to 350 nm for 25 different mixtures of MB and TU. The calibration curve was linear across the concentration range of 1 to 10 μg mL and 1.5 to 15 μg mL for MB and TU, respectively. The values of the root mean squares error of prediction (RMSEP) were 0.3984, 0.1066, and 0.0713 for MB and 0.2010, 0.1667, and 0.1115 for TU, which were obtained using PCR, PLS, and GA‐PLS, respectively. Finally, the practical applicability of the GA‐PLS method was effectively evaluated by the concurrent detection of both analytes in animal tissues. It should also be mentioned that the proposed method is a simple and rapid way that requires no preliminary separation steps and can be used easily for the analysis of these compounds, especially in quality control laboratories.
language:
source:
identifier: ISSN: 0022-1147 ; E-ISSN: 1750-3841 ; DOI: 10.1111/j.1750-3841.2009.01458.x
fulltext: fulltext
issn:
  • 0022-1147
  • 00221147
  • 1750-3841
  • 17503841
url: Link


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titleSimultaneous Spectrophotometric Determination of 2‐Thiouracil and 2‐Mercaptobenzimidazole in Animal Tissue Using Multivariate Calibration Methods: Concerns and Rapid Methods for Detection
creatorBeheshti, Abolghasem ; Riahi, Siavash ; Pourbasheer, Eslam ; Ganjali, Mohammad Reza ; Norouzi, Parviz
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descriptionTwo multivariate calibration methods, partial least squares (PLS) and principal component regression (PCR), were applied to the spectrophotometric simultaneous determination of 2‐mercaptobenzimidazole (MB) and 2‐thiouracil (TU). A genetic algorithm (GA) using partial least squares was successfully utilized as a variable selection method. The concentration model was based on the absorption spectra in the range of 200 to 350 nm for 25 different mixtures of MB and TU. The calibration curve was linear across the concentration range of 1 to 10 μg mL and 1.5 to 15 μg mL for MB and TU, respectively. The values of the root mean squares error of prediction (RMSEP) were 0.3984, 0.1066, and 0.0713 for MB and 0.2010, 0.1667, and 0.1115 for TU, which were obtained using PCR, PLS, and GA‐PLS, respectively. Finally, the practical applicability of the GA‐PLS method was effectively evaluated by the concurrent detection of both analytes in animal tissues. It should also be mentioned that the proposed method is a simple and rapid way that requires no preliminary separation steps and can be used easily for the analysis of these compounds, especially in quality control laboratories.
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abstractTwo multivariate calibration methods, partial least squares (PLS) and principal component regression (PCR), were applied to the spectrophotometric simultaneous determination of 2‐mercaptobenzimidazole (MB) and 2‐thiouracil (TU). A genetic algorithm (GA) using partial least squares was successfully utilized as a variable selection method. The concentration model was based on the absorption spectra in the range of 200 to 350 nm for 25 different mixtures of MB and TU. The calibration curve was linear across the concentration range of 1 to 10 μg mL and 1.5 to 15 μg mL for MB and TU, respectively. The values of the root mean squares error of prediction (RMSEP) were 0.3984, 0.1066, and 0.0713 for MB and 0.2010, 0.1667, and 0.1115 for TU, which were obtained using PCR, PLS, and GA‐PLS, respectively. Finally, the practical applicability of the GA‐PLS method was effectively evaluated by the concurrent detection of both analytes in animal tissues. It should also be mentioned that the proposed method is a simple and rapid way that requires no preliminary separation steps and can be used easily for the analysis of these compounds, especially in quality control laboratories.
copMalden, USA
pubBlackwell Publishing Inc
doi10.1111/j.1750-3841.2009.01458.x
pagesC135-C139
date2010-03